A5071791155- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Probiotics and Fermented Foods
- Salmonella and Campylobacter epidemiology
- Proteins in Food Systems
- Genomics and Phylogenetic Studies
- Protein Hydrolysis and Bioactive Peptides
- Infant Nutrition and Health
- Cancer Genomics and Diagnostics
- Gut microbiota and health
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
Wageningen University & Research
2021-2024
Microbial population heterogeneity leads to different stress responses and growth behavior of individual cells in a population. Previously, point mutation the rpsU gene (rpsUG50C) encoding ribosomal protein S21 was identified Listeria monocytogenes LO28 variant, which increased multi-stress resistance reduced maximum specific rate. However, underlying mechanisms these phenotypic changes remain unknown. In L. monocytogenes, alternative sigma factor SigB regulates general response, with its...
Population heterogeneity is an important component of the survival mechanism Listeria monocytogenes, leading to cells in a population with diverse stress resistance levels. We previously demonstrated that several ribosomal gene rpsU mutations enhanced L. monocytogenes and lowered growth rate at 30 °C lower temperatures. This study investigated whether these switches phenotypes could result bias strain detection when standard enrichment-based procedures are applied variety strains. Detailed...
Abstract The pan-genome consists of core genes shared by all members a taxonomy and accessory found in only subset, holding the keys to advancing our understanding evolution tackling medical challenges. Here, we discovered strong intra-genomic correlation among bacterial within each Escherichia coli , Listeria monocytogenes Staphylococcus aureus Campylobacter jejuni . With convolutional neural network assisted genome knock-out simulation, that different combinations fewer than 20 highly...
Microbial population heterogeneity contributes to differences in stress response between individual cells a population, and can lead the selection of genetically stable variants with increased resistance. We previously provided evidence that multiple-stress resistant Listeria monocytogenes LO28 variant 15, carries point mutation rpsU gene, resulting an arginine-proline substitution ribosomal protein RpsU (RpsU17Arg-Pro). Here, we investigated trade-off general sigma factor SigB-mediated...
Multiple stress resistant variants of
Listeria monocytogenes, a widespread food-borne pathogen, utilizes diverse growth substrates including mono- and di-saccharides via PEP-phosphotransferase (PTS) systems. We evaluated collection of L. monocytogenes isolates different origins for their ability to utilize lactose, disaccharide composed galactose glucose the main carbon source in milk dairy products. Notably, dairy-associated outbreak strain F2365 could not lactose efficiently conceivably due frameshift mutation (lacR887del)...
Glycation elicits diverse effects on the digestion of caseins and whey proteins in a goat protein model system (40 % casein,60 proteins). Caseins generated longer peptides digesta heated vs unheated samples. The further cleavage casein was hindered by dry heating, which might be induced glycated modification. This reduced may also explain decreased intensity casein-derived bioactive peptides. Moreover, heating for 72 h significantly total number protein-derived Whey samples were shorter than...
Population heterogeneity is an important component of the survival strategy Listeria monocytogenes, leading to cells in a population with diverse stress resistance levels. We previously demonstrated that ribosomal gene rpsU mutations enhanced L. monocytogenes and lowered growth rate at 30°C lower temperatures. This study investigated whether these switches phenotypes could result bias strain detection when standard enrichment-based procedures are applied variety strains. Detailed kinetics...