A5090478455- Preterm Birth and Chorioamnionitis
- Bladder and Urothelial Cancer Treatments
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Hormonal Regulation and Hypertension
- Bacterial biofilms and quorum sensing
- Single-cell and spatial transcriptomics
- Bacterial Genetics and Biotechnology
- Reproductive System and Pregnancy
- Urological Disorders and Treatments
- Microbial Community Ecology and Physiology
- Estrogen and related hormone effects
- Urinary Tract Infections Management
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Retinal Development and Disorders
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pregnancy and preeclampsia studies
- Tissue Engineering and Regenerative Medicine
- Photoreceptor and optogenetics research
- Ubiquitin and proteasome pathways
Yunnan University
2020-2025
Center for Life Sciences
2023-2025
Wuhan University
2024
Wuhan Institute of Virology
2024
State Key Laboratory of Virology
2024
North Sichuan Medical University
2024
Harvard University
2014-2023
Boston Children's Hospital
2013-2023
Cedars-Sinai Medical Center
2022-2023
Fudan University
2007-2016
FOXA1 is a nonhistone substrate of EZH2, promoting cell growth and sensitizing some prostate cancer to enzymatic EZH2 inhibitors.
The induction of cyclooxygenase-2 (COX-2) and subsequent production prostaglandin E2 (PGE2) by cortisol in the amnion contrast with effect on most other tissues, but this proinflammatory may be a key event human parturition (labor). We evaluated underlying mechanism activated primary fibroblasts. Exposure fibroblasts to led activation cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway, which induced phosphorylation SRC STAT3 (signal transducer activator transcription 3)....
The amount of cortisol available to its receptors is increased by the pre-receptor enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) which converts cortisone cortisol. We examined molecular mechanisms feedback effect on 11beta-HSD1 mRNA expression in human amnion fibroblasts. Our data showed that cortisol-induced dose dependently fibroblasts, could be completely blocked both transcription inhibitor 5,6-dichlorobenzimidazole riboside and glucocorticoid receptor (GR) antagonist...
Maternal nutrient reduction (MNR) during fetal development may predispose offspring to chronic disease later in life. Increased regeneration of active glucocorticoids by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) metabolic tissues is fundamental the developmental programming syndrome, but underlying mechanisms are unknown. Hexose-6-phosphate (H6PD) generates NADPH, cofactor for 11β-HSD1 reductase activity. CCAAT/enhancer binding proteins (C/EBPs) and glucocorticoid receptor (GR)...
Urofacial syndrome (UFS) is an autosomal recessive disease with severe dysfunctional urination including urinary incontinence (UI). Biallelic mutations of HPSE2 are discovered from UFS patients, suggesting that a candidate gene. Here, we show deletion Hpse2 sufficient to cause the UFS-like phenotype in mice. knockout mutants display distended bladder (megacystis) and abnormal voiding behavior similar found patients. While largely dispensable for detrusor smooth muscle urothelial cell fate...
In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating amidst the prevalent rRNA during single-cell RNA-seq. This study introduces novel method, ribosomal RNA-derived cDNA depletion (RiboD), seamlessly integrated into PETRI-seq technique, yielding RiboD-PETRI. innovative approach offers cost-effective, equipment-free, and high-throughput solution for RNA sequencing (scRNA-seq). By efficiently eliminating reads substantially enhancing...
Overexposure of the fetus to glucocorticoids early in gestation is detrimental fetal development. Glucocorticoid concentrations circulation are kept low by 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2, encoded HSD11B2) placental syncytiotrophoblasts. However, cytotrophoblasts, progenitors syncytiotrophoblasts, express levels 11β-HSD2. Here we studied molecular mechanisms underlying 11β-HSD2 induction upon syncytialization. Freshly isolated human term cytotrophoblasts and vitro...
Introduction: The endothelial glycocalyx degrading enzyme heparanase-1 (HPSE1) is a major contributor to kidney diseases, such as glomerulonephritis and diabetic nephropathy. Therefore, inhibition of HPSE1 could be an interesting therapeutic strategy treat glomerular diseases. A possible inhibitor heparanase-2 (HPSE2) because HPSE2 structural homolog without enzymatic activity. importance has been recently demonstrated in HPSE2-deficient mice, since these mice developed albuminuria died...
Human amnion fibroblasts produce abundant prostaglandins toward the end of gestation, which is one major events leading to parturition. In marked contrast its well-described antiinflammatory effect, glucocorticoids have been shown up-regulate cyclooxygenase-2 (COX-2) expression in human fibroblasts. The mechanisms underlying this paradoxical induction COX-2 by not resolved. Using cultured fibroblasts, we found that mRNA cortisol was a glucocorticoid receptor (GR)-dependent process requiring...
Abundant evidence indicates a pivotal role of prostaglandin F2α (PGF2α) in human parturition. Both the fetal and maternal sides membranes synthesize PGF2α. In addition to synthesis PGF2α from PGH2 by PGF synthase (PGFS), can also be converted PGE2 carbonyl reductase 1 (CBR1). Here, we showed that there was concurrent increased production cortisol association with elevation CBR1 amnion obtained at term labor versus without labor. cultured primary fibroblasts, (0.01–1μM)...
The mechanisms underlying human parturition are still not understood, yet we need this knowledge to combat preterm birth. Fetal membranes express abundant 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1), which converts inert cortisone active cortisol. We examined whether cortisol regeneration in the amnion might play a role through regulation of lysyl oxidase (LOX), collagen cross-linking enzyme, thereby contributing rupture fetal membranes. By using cultured primary fibroblasts, demonstrated...
Abstract Recurrent urinary tract infections (UTIs) are increasingly associated with antibiotic resistance, posing significant clinical challenges. This study evaluates the antimicrobial efficacy of membrane-intercalating conjugated oligoelectrolyte COE-D8 against uropathogenic Escherichia coli (UPEC). Among 93 UPEC isolates, over 50% demonstrated resistance to first-line antibiotics, compound sulfamethoxazole showing highest (75% at a minimum inhibitory concentration (MIC) >512 μg/mL)....
ABSTRACT Inherited retinal degeneration (IRD) is a debilitating condition characterized by progressive loss of photoreceptor cells. However, the underlying mechanisms remain largely unclear. This study investigated role DNA topoisomerase II alpha (TOP2A) and its interplay with protein kinase G (PKG) histone deacetylase (HDAC) in rd1 mouse model for IRD. Immunofluorescence quantitative western blotting analyses were performed to evaluate expression TOP2A, PKG1, PKG2, HDAC1, other related...
e16547 Background: Poliovirus receptor (PVR) is a ligand for TIGIT and plays crucial role in tumor immune evasion. We have identified that PVR highly expressed bladder cancer (BC), particularly cases are resistant to immunotherapy. This study aims investigate whether antibody-drug conjugate (ADC) targeting can provide both antitumor activity inhibition of escape BC. Methods: Forty-one BC patients who received anti-PD1 neoadjuvant therapy were analyzed identify proteins linked immunotherapy...
Three-dimensional cell tissue culture, which produces biological structures termed organoids, has rapidly promoted the progress of research, including basic drug discovery, and regenerative medicine. However, due to lack algorithms software, analysis organoid growth is labor intensive time-consuming. Currently it requires individual measurements using software such as ImageJ, leading low screening efficiency when used for a high throughput screen. To solve this problem, we developed bladder...
Compelling evidence indicates a crucial role of prostaglandin F2α (PGF2α) in parturition. Both the maternal and fetal sides membranes synthesize PGF2α, which exerts effects via receptor (FP) that is coupled to activation protein kinase C (PKC). Cyclooxygenase-2 (COX-2) catalyzes rate-limiting step inducible synthesis prostaglandin. Although PKC known induce COX-2 expression, it not clear whether PGF2α can FP receptor-coupled activation. promoter carries cAMP-response element (CRE)...
Urothelium is the protective lining of urinary tract. The mechanisms underlying urothelial formation and maintenance are largely unknown. Here, we report stage-specific roles PRC2 epigenetic regulators in embryonic adult progenitors. Without Eed, obligatory subunit PRC2, progenitors demonstrate reduced proliferation with concomitant dysregulation genes including Cdkn2a (p16), Cdkn2b (p15) Shh. These mutants display premature differentiation keratin 5-positive (Krt5+) basal cells ectopic...
To understand how brain regions form and work, it is important to explore the spatially variable genes (SVGs) enriched in specific during development. Spatial transcriptomics techniques provide opportunity select SVGs high-throughput way. However, previous methods neglected ranking order combinatorial effect of SVGs, making them difficult automatically high-priority from spatial data. Here, we proposed a novel computational pipeline, called SVGbit, rank individual for marker selection...