A5101787658- 3D Printing in Biomedical Research
- Salivary Gland Disorders and Functions
- Hair Growth and Disorders
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Liver Disease Diagnosis and Treatment
- melanin and skin pigmentation
- Diet, Metabolism, and Disease
- Proteoglycans and glycosaminoglycans research
- Polymer composites and self-healing
- Diet and metabolism studies
- Lignin and Wood Chemistry
- Biochemical Analysis and Sensing Techniques
- Dermatologic Treatments and Research
- Skin Protection and Aging
- Carbon dioxide utilization in catalysis
National Cancer Institute
2024-2025
National Institutes of Health
2024
Center for Cancer Research
2024
University of Louisville
2024
Mississippi State University
2019
Royal Hallamshire Hospital
1991-1994
Abstract Three-dimensional (3D) in vitro cell culture models are invaluable tools for investigating the tumor microenvironment (TME). However, analyzing impact of critical stromal elements, such as extracellular matrix (ECM), remains a challenge. Here, we developed hydrogel-free self-assembly platform to establish ECM-rich 3D “MatriSpheres” deconvolute cancer cell-ECM interactions. Mouse and human colorectal (CRC) MatriSpheres actively incorporated microgram quantities decellularized small...
Journal Article Extracellular matrix derived from hair and skin fibroblasts stimulates human melanocyte tyrosinase activity Get access J.A. BUFFEY, BUFFEY University Department of Medicine, Clinical Sciences Centre, Northern General Hospital, Sheffield S5 7AU, U.K. Search for other works by this author on: Oxford Academic Google Scholar A.G. MESSENGER, MESSENGER Dermatology, Royal Hallamshire S10, M. TAYLOR, TAYLOR A.T.T. ASHCROFT, ASHCROFT G.E. WESTGATE, WESTGATE Unilever Research, Colworth...
Abstract The tumor microenvironment consists of resident cells organized within a compositionally diverse, three-dimensional (3D) extracellular matrix (ECM) network that cannot be replicated in vitro using bottom-up synthesis. We report new self-assembly system to engineer ECM-rich 3D MatriSpheres wherein actively organize and concentrate microgram quantities decellularized ECM dispersions which modulate cell phenotype. colorectal cancer (CRC) were created small intestine submucosa (SIS) as...
The extracellular matrix of the hair follicle dermal papilla is rich in glycosaminoglycans, expression which varies during growth cycle being maximal anagen and becoming undetectable as enters telogen. These observations, together with other experimental clinical evidence, suggest that glycosaminoglycans may be involved regulating growth. To investigate metabolism by we have measured incorporation radiolabelled precursors into released culture medium cultured human cells. We also studied...
Background: Intestine epithelial hypoxia-inducible factor-1α (HIF-1α) plays a critical role in maintaining gut barrier function. The aim of this study was to determine whether pharmacological or genetic activation intestinal HIF-1α ameliorates western diet–induced metabolic dysfunction–associated steatotic liver disease. Methods: Metabolic effects by dimethyloxalylglycine were evaluated HIF-α luciferase reporter (ODD-luc) mice. Male and/or female epithelial–specific Hif1α overexpression mice...