A5100340398- Cancer-related molecular mechanisms research
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Forensic and Genetic Research
- Inflammatory Bowel Disease
- Immunodeficiency and Autoimmune Disorders
- Molecular Biology Techniques and Applications
- Estrogen and related hormone effects
- Adipose Tissue and Metabolism
- Animal Genetics and Reproduction
- Phytochemistry and biological activities of Ficus species
- Saffron Plant Research Studies
- Helicobacter pylori-related gastroenterology studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Heart Rate Variability and Autonomic Control
- Autoimmune and Inflammatory Disorders
- RNA and protein synthesis mechanisms
- Genetic diversity and population structure
- Transplantation: Methods and Outcomes
- Gut microbiota and health
- Meat and Animal Product Quality
- Horticultural and Viticultural Research
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
Sichuan University
2023
Sichuan Animal Science Academy
2020-2022
Jiangxi University of Traditional Chinese Medicine
2022
First Affiliated Hospital of Soochow University
2021
Ruijin Hospital
2016-2021
Shanghai Jiao Tong University
2021
Sichuan Agricultural University
2021
Soochow University
2021
China University of Political Science and Law
2015-2017
Tianjin Medical University
2010
The present study aimed to investigate the role and underlying mechanisms of long non-coding RNA (lncRNA) muscleblind-like 1 antisense (MBNL1-AS1) in progression Prostate cancer (PCa). MBNL1-AS1 microRNA (miR)-181a-5p expression PCa tissues several human cell lines were analyzed, respectively, using StarBasev3.0 project RT-qPCR assay. After overexpression, proliferation, invasion migration were, evaluated CCK-8, colony formation, transwell wound healing assays. Dual luciferase assay used for...
AIM:To perform sequencing analysis in patients with very early-onset inflammatory bowel disease (VEO-IBD) to determine the genetic basis for VEO-IBD Chinese pediatric patients. METHODS:A total of 13 were diagnosed from May 2012 and August 2014.The relevant clinical characteristics these analyzed.Then DNA peripheral blood was extracted.Next generation (NGS) based on an Illumina-Miseq platform used analyze exons coding regions 10 candidate genes: IL-10 , IL-10RA IL-10RB NOD2 FUT2 IL23R GPR35...
The whole-genome sequencing (WGS) data can potentially discover all genetic variants. Studies have shown the power of WGS for genome-wide association study (GWAS) lies in ability to identify quantitative trait loci and nucleotides (QTNs). However, resequencing thousands target individuals is expensive. Genotype imputation a powerful approach causal mutations. This aimed evaluate accuracy from genotyping-by-sequencing (GBS) two pig breeds using reference population detect single-nucleotide...
The Chinese Qingyu pig breed is an invaluable indigenous genetic resource. However, few studies have investigated the architecture of meat quality traits in pigs. Here, 30 purebred pigs were subjected to whole-genome sequencing. After control, 18 436 759 SNPs retained. Genome-wide association (GWAS) then performed for pH and color at three postmortem time points (45 min, 24 h, 48 h) using single-marker regression analysis. In total, 11 69 associated with longissimus thoracis muscle (LTM),...
The Chinese Chenghua pig (CHP) is a typical domestic fatty breed with superior meat quality characteristics, while the Yorkshire (YP) has characteristics of fast growth and high rate lean meat. Long term natural selection artificial resulted in great phenotypic differences between two breeds, including growth, development, production performance, quality, coat color. However, genome-wide DNA methylation CHP YP remain unclear.DNA data were generated for muscle tissues using reduced...
miRNA is a small non-coding RNA, which plays an important role in diverse biological processes. In the present study, we explore effect of ssc-miR-451 on porcine adipose development and meat quality. We observed that was downregulated during primary adipocyte differentiation. Overexpression inhibited adipogenic differentiation, while inhibition promoted The dual luciferase reporter system indicated Acetyl-CoA carboxylase alpha (ACACA) as target gene ssc-miR-451. Correlation analysis...
Approximately 25-40% of patients with CD can have multiple extraintestinal manifestations, including erythema nodosum, pyoderma gangrenosum, ankylosing spondylitis, arthritis, hepatitis, sclerosing cholangitis, pancreatitis, nephrolithiasis, uveitis, and episcleritis (2).The first report lung involvement in was published 1976 (3).The bronchopulmonary manifestations are very rare the literature reports that prevalence these is as low 0.2% (4).However, this rate may be underestimated, an...
Birth weight (BW) and days to 100 kg (D100) are important economic traits that both affected by polygenes. However, the genetic architecture of these quantitative is still elusive. Genotyping-by-sequencing (GBS) data containing a large number single nucleotide polymorphisms (SNPs) have become powerful tool in genomic analysis. To better understand their complex structure, total 600 Yorkshire pigs were sequenced using GBS technology. After quality control, 279 787 SNPs generated for...
Interleukin 10 receptor alpha subunit (IL10RA) dysfunction is the main cause of very early-onset inflammatory bowel disease (VEO-IBD) in East Asians.To identify disease-causing gene mutations four patients with VEO-IBD and verify functional changes related to mutations.From May 2016 September 2020, young clinically diagnosed were recruited. Before hospitalization, using targeted panel sequencing trio-whole-exome (WES), three found harbor a IL10RA mutation (c.301C>T, p.R101W one patient;...
The gardenia is a traditional medicinal horticultural plant in China, but its molecular genetic research has been largely hysteretic. Here, we constructed an F1 population with 200 true hybrid individuals. Using the genotyping-by-sequencing method, high-density sex-average map was generated that contained 4,249 SNPs total length of 1956.28 cM and average distance 0.46 cM. We developed 17 SNP-based Kompetitive Allele-Specific PCR markers found 15 were successfully genotyped, which 13...
China has the largest pork consumption worldwide. However, high incidence of piglet fetal mummification (3%-5%) is an important factor that causes slow improvement pig reproductive capacity, and genetic mechanism still unclear. This study aimed to identify candidate genes associated with mummification. RNA-seq technology was used compare transcriptome profiling blood from healthy mummified piglets at different stages pregnancy (35, 56, 77, 98 days). A total 137-420 differentially expressed...
Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) candidate genes number mummified (NM) piglets using imputed whole-genome sequence (WGS) validate potential genes.The WGS was introduced genotyping-by-sequencing (GBS) multi-breed reference population. We performed...
The Chinese Qingyu pig is a typical domestic fatty breed and an invaluable indigenous genetic resource in China. Compared with the Landrace pig, has unique meat characteristics, including muscle development, intramuscular fat, other quality traits. At present, few studies have explored epigenetic differences due to DNA methylation between pig. In this study, 30 pigs 31 were subjected reduced representation bisulfite sequencing (RRBS). Genome-wide differential analysis was conducted. Six...