A5017494680- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Horticultural and Viticultural Research
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Fermentation and Sensory Analysis
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Genomics and Chromatin Dynamics
- Fungal Plant Pathogen Control
- Prenatal Screening and Diagnostics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Immunodeficiency and Autoimmune Disorders
- Genetics and Neurodevelopmental Disorders
- Insect symbiosis and bacterial influences
- Glycosylation and Glycoproteins Research
- Congenital heart defects research
- Plant and Fungal Interactions Research
- Single-cell and spatial transcriptomics
- Microbial Metabolism and Applications
- Mosquito-borne diseases and control
- Forensic and Genetic Research
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
Cereal Research Centre
2023-2025
University of Bari Aldo Moro
2018-2023
Centro di Ricerca per l’Orticoltura
2021-2022
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
2021-2022
A high-quality rhesus macaque genome Genome technology has improved substantially since the first full organismal genomes were generated. Applying new technology, Warren et al. refined of macaque, a model nonhuman primate. Long-read and other recent advances in sequencing applied to generate with far fewer gaps helped refine locations numbers repetitive elements. Furthermore, authors performed resequencing among populations identify genetic variability macaque. Thus, previously incomplete...
Unlike copy number variants (CNVs), inversions remain an underexplored genetic variation class. By integrating multiple genomic technologies, we discover 729 in 41 human genomes. Approximately 85% of <2 kbp form by twin-priming during L1 retrotransposition; 80% the larger are balanced and affect twice as many nucleotides CNVs. Balanced show excess common variants, 72% flanked segmental duplications (SDs) or retrotransposons. Since flanking repeats promote non-allelic homologous...
Adaptive archaic hominin genes As they migrated out of Africa and into Europe Asia, anatomically modern humans interbred with hominins, such as Neanderthals Denisovans. The result this genetic introgression on the recipient populations has been considerable interest, especially in cases selection for specific variants. Hsieh et al. characterized adaptive structural variants copy number that are likely targets positive Melanesians. Focusing population-specific regions genome carry duplicated...
Grapevine (Vitis vinifera L.), a globally significant crop, is highly susceptible to Botrytis cinerea, the causative agent of gray mold disease. This study investigates transcriptomic responses B. cinerea in tolerant and grapevine genotypes using RNA sequencing (RNA-seq). Differentially expressed genes (DEGs) were identified at three time points (T1, T2, T3), highlighting both genotype-independent genotype-specific responses. Early-stage infection (T1) revealed rapid robust activation...
Post-harvest decay of fresh table grapes causes considerable annual production losses. The main fungal agents both in pre- and post-harvest are B. cinerea, Penicillium spp., Aspergillus Alternaria Cladosporium spp. To date, the use agrochemicals SO2 methods to control grape molds postharvest, respectively. Significant improvements, however, have already been made apply innovative more environmentally sustainable strategies, such as Biological Control Agents (BCAs), which can reduce disease...
For many years, inversions have been proposed to be a direct driving force in speciation since they suppress recombination when heterozygous. Inversions are the most common large-scale differences among humans and great apes. Nevertheless, represent large events easily distinguishable by classical cytogenetics, whose resolution, however, is limited. Here, we performed genome-wide comparison between human, ape, macaque genomes using net alignments for recent releases of genome assemblies. We...
DNA-free genome editing by the direct delivery of CRISPR-associated nucleases has emerged as a promising technology due to its precision and reduced risk off-target effects. However, existing purification protocols for native Cas proteins require use complex instrumentation, which limits their application. Here, we present simplified protocol Cas9, Cas12RR dCas9-VP64 optimized editing. Our approach leverages streamlined affinity ion exchange chromatography coupled with minimal downstream...
It is known that plant-associated microorganisms must be considered as key drivers for plant health, productivity, community composition, and ecosystem functioning. Much attention has been paid to the exploration of microbiome in search new with antagonistic activity against various phytopathogens. P. viticola represents one main fungal agents grape yield losses during pre-harvest stage. To date, use chemical pesticides method control downy mildew infection vineyards. In recent years,...
Extracting high-quality RNA from grape (Vitis Vinifera) berry skins is challenging due to their high levels of polysaccharides, phenolic compounds, sugars, and organic acids, which can negatively impact purity yield. Indeed, polyphenols bind RNA, polysaccharides may co-precipitate, sugars acids interfere with the pH ionic properties extraction buffer. Commercial kits offer a quick method but are often ineffective for skins. Similarly, protocols that work well other vegetal tissues also...
Postharvest spoilage fungi, such as Botrytis cinerea, are considered the main cause of losses fresh fruit quality and vegetables during storage, distribution, consumption. The current control strategy is use SO2 generator pads whose application now largely under observation. A high quantity can be deleterious for fruits it not allowed in organic agriculture. For this reason, great attention has been recently focused on identifying Biological Control Agents (BCA) to implement biological...
Human chromosome 15q25 is involved in several disease-associated structural rearrangements, including microdeletions and chromosomal markers with inverted duplications. Using comparative fluorescence situ hybridization, strand-sequencing, single-molecule, real-time sequencing Bionano optical mapping analyses, we investigated the organization of region human nonhuman primates. We found that two independent inversions occurred this after fission event gave rise to phylogenetic chromosomes XIV...
Rhesus macaque is an Old World monkey that shared a common ancestor with human ∼25 Myr ago and important animal model for disease studies. A deep understanding of its genetics therefore required both biomedical evolutionary Among structural variants, inversions represent driving force in speciation play role predisposition. Here we generated genome-wide map between macaque, combining single-cell strand sequencing cytogenetics. We identified 375 total 859 bp 92 Mbp, increasing by eightfold...
The impact of segmental duplications on human evolution and disease is only just starting to unfold, thanks advancements in sequencing technologies that allow for their discovery precise genotyping. 15q11-q13 locus a hotspot recurrent copy number variation associated with Prader-Willi/Angelman syndromes, developmental delay, autism, epilepsy mediated by complex duplications, many which arose recently during evolution. To gain insight into the instability this region, we characterized its...
Abstract Unlike copy number variants (CNVs), inversions remain an underexplored genetic variation class. By integrating multiple genomic technologies, we discover 729 in 41 human genomes. Approximately 85% of <2 kbp form by twin-priming during L1-retrotransposition; 80% the larger are balanced and affect twice as many base pairs CNVs. Balanced show excess common variants, 72% flanked segmental duplications (SDs) or mobile elements. Since this suggests recurrence due to non-allelic...
Gibbons are the most speciose family of living apes, characterized by a diverse chromosome number and rapid rate large-scale rearrangements. Here we performed single-cell template strand sequencing (Strand-seq), molecular cytogenetics, deep in silico analysis southern white-cheeked gibbon genome, providing first comprehensive map 238 previously hidden small-scale inversions. We determined that more than half specific, at least fivefold higher shown for other primate lineage-specific...
POTE (prostate, ovary, testis, and placenta expressed) genes belong to a primate-specific gene family expressed in prostate, testis as well several cancers including breast, lung cancers. Due their tumor-specific expression, POTEs are potential oncogenes, therapeutic targets, biomarkers for these malignancies. This maps within human primate segmental duplications with copy number ranging from two 14 different species. the high sequence identity among copies, specific efforts needed assemble...
New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation for grapes are based on the generation/induction of embryogenic callus cells that then transformed. Varieties such as Italia proven be very recalcitrant regeneration via somatic embryogenesis. In this work, development a protocol production calluses is described. Two sterilization were tested: (a) lower active...
Vitis vinifera is an important fruit crop which mainly consumed fresh or used for the production of wine. Genetic improvement programs through New Genomic Techniques (NGTs) aim to develop grapevine varieties resistant biotic and abiotic stresses enhancing nutraceutical properties. In order apply NGTs, maintaining embryogenic calluses from flower tissues critical. Optimizing culture conditions—pH, gelling agents, temperature, light, growth regulators, gas composition—is essential inducing...
Chromosome deletions, including band 5q12, have rarely been reported and associated with a wide range of clinical manifestations, such as postnatal growth retardation, intellectual disability, hyperactivity, nonspecific ocular defects, facial dysmorphism, epilepsy. In this study, we describe for the first time child retardation in which identified balanced t(3;10) translocation by conventional cytogenetic analysis addition to an 8.6 Mb 5q12 deletion through array-CGH. Our results show that...