A5028364627- Cholinesterase and Neurodegenerative Diseases
- Chemical synthesis and alkaloids
- Neural Networks and Applications
- Plant-Microbe Interactions and Immunity
- Allelopathy and phytotoxic interactions
- Fuzzy Logic and Control Systems
- Computational Drug Discovery Methods
- Biofuel production and bioconversion
- Monoclonal and Polyclonal Antibodies Research
- Plant Pathogens and Fungal Diseases
- GABA and Rice Research
- HER2/EGFR in Cancer Research
- Potato Plant Research
- Transportation and Mobility Innovations
- Vehicle Routing Optimization Methods
- Medicinal Plants and Neuroprotection
- Microbial Community Ecology and Physiology
- Time Series Analysis and Forecasting
- Data Visualization and Analytics
- Toxin Mechanisms and Immunotoxins
- Legume Nitrogen Fixing Symbiosis
- Smart Parking Systems Research
- Genomics and Phylogenetic Studies
- Forest Management and Policy
- Forest Biomass Utilization and Management
Tokyo University of Agriculture
2011-2024
Toyohashi University of Technology
2024
University Hospital Kyoto Prefectural University of Medicine
2023
Kyoto Prefectural University of Medicine
2004-2022
Mie University
2001-2022
Kochi University of Technology
2020
Canon (Japan)
2018
Kiel University
2010-2017
Advanced Life Science Institute
2015
Fukuoka University
2013
An anaerobic mesophile, Clostridium cellulovorans, produces a multienzyme complex called the cellulosome and actively degrades polysaccharides in plant cell wall. C. cellulovorans also changes cellulosomal subunits to form highly active combinations dependent on carbon substrate. A previous study reported synergistic effects of exoglucanase S (ExgS) endoglucanase H (EngH) that are classified into glycosyl hydrolase (GH) families 48, 9, respectively. In this study, we investigated ExgS EngK,...
Signaling of the cytokine interleukin-6 (IL-6) via its soluble IL-6 receptor (sIL-6R) is responsible for proinflammatory properties and constitutes an attractive therapeutic target, but how sIL-6R generated in vivo remains largely unclear. Here, we use liquid chromatography-mass spectrometry to identify form human serum that originates from proteolytic cleavage, map cleavage site between Pro-355 Val-356, determine occupancy all O- N-glycosylation sites sIL-6R. The metalloprotease a...
X-linked inhibitor of apoptosis protein (XIAP) is the most potent caspase-inhibitory IAP family member and a negative regulator various apoptotic stimuli. Thus, XIAP overexpression in cancer cells may select for tumor cell survival following cytotoxic therapeutic modalities. The anatomical staging system renal carcinoma (RCC) currently provides good prognostic information, albeit insufficient. We hypothesize that RCC serve as molecular marker improve RCC. This study examined level lysates...
Root-associated microbial communities are very important in the adaptation of halophytes to coastal environments. However, little has been reported on community structures related halophytes, or comparisons their compositions among halophytic plant species. Here, we studied diversity and structure both rhizosphere root endosphere bacteria two plants: Glaux maritima Salicornia europaea. We sampled rhizosphere, endosphere, bulk control soil samples, performed bacterial 16S rRNA sequencing...
Purpose Second mitochondria-derived activator of caspase/direct inhibitor apoptosis-binding protein with low pI (Smac/DIABLO) was recently identified as a that is released from mitochondria in response to apoptotic stimuli and promotes apoptosis by antagonizing proteins. Furthermore, Smac/DIABLO plays an important regulatory role the sensitization cancer cells both immune- drug-induced apoptosis. However, little known about clinical significance various cancers, including renal cell...
Abstract Acetylcholinesterase (AChE) has been increasingly recognized in plants by indirect evidence of its activity. Here, we report purification and cloning AChE from maize (Zea mays), thus providing to our knowledge the first direct molecule plants. was identified as a mixture disulfide- noncovalently linked 88-kD homodimers consisting 42- 44-kD polypeptides. The hydrolyzed acetylthiocholine propyonylthiocholine, but not S-butyrylthiocholine, AChE-specific inhibitor neostigmine bromide...
ADAM17 (A disintegrin and metalloproteinase 17) is a membrane-bound protease that cleaves various cell surface proteins, including cytokines cytokine receptors. Recently it was shown highly expressed on the of many cancer cells, whereas normal cells express low levels ADAM17, implying potential immunotherapeutic target. We have generated monoclonal antibody against human which recognized membrane proximal cysteine-rich extension protein. Unlike tumour lines, such as prostate line, pancreatic...
In this work a method for using accelerometers the determination of angular velocity and acceleration is presented. Minimum sensor requirements insights into how an array can be configured to maximize estimator performance are considered. The framework presented utilizes linear least squares estimate functions that quadratic in velocity. Simple methods determining sign spin axis linearized covariance approximation found perform quite effectively when compared results obtained by Monte Carlo.
The Nurse Scheduling Problem (NSP) is a problem of allocating shifts (day and night shifts, holidays, so on) for nurses under various constraints. Generally, NSP has lot As result, it needs knowledge experience to construct the scheduling table with its constraints, usually done by head nurse or authority in hospitals. Some research on using genetic algorithms (GA) reported. Conventional methods take constraints into fitness function. However, if reduces value parts solution against causes...
Abstract BACKGROUND Tumor necrosis factor–related apoptosis‐inducing ligand (TRAIL) plays an important role in the process of lymphocyte‐mediated cytotoxicity against malignant cells. Osteoprotegerin (OPG) is a soluble decoy receptor for TRAIL, and circulating OPG has been implicated protection cells from TRAIL‐mediated apoptosis. Thus, may protect tumor and, as result, contribute to progression. In current study, authors investigated this hypothesis patients with bladder carcinoma. METHODS...
"Salicornia europaea" increases acetylcholinesterase (AChE) accompanied by salt accumulation during their growth. The plant acetylcholine (ACh)-mediated system in Salicornia could be responsible for transport of ions through channels a manner similar to the animal systems. In this study, AChE gene was identified RT-PCR using degenerate primers designed based on previously cloned maize and siratro genes. full-length cDNA 1,536 nucleotides, encoding 387-residue protein that includes 28-residue...
Interleukin-6 (IL-6) is a cytokine largely induced during infection, inflammation, and cancer. In the liver, IL-6 induces synthesis of acute-phase proteins, which are believed to support response body infection inflammation. Moreover, has been reported be growth factor in multiple myeloma. on cells binds an receptor (IL-6R) forming IL-6/IL-6R complex, associates with homodimer second subunit, gp130, initiate signaling. Gp130 present all body, whereas IL-6R only expressed hepatocytes, some...
Summary The ascomycete fungus Magnaporthe oryzae is a hemibiotrophic pathogen that causes rice blast disease. infects leaves, stems and panicles, induces severe reductions in yield. Effector proteins secreted by M. planta are thought to be involved its virulence activity. Here, using RNA‐sequencing (RNA‐Seq), we generated transcriptome data for isolate Ina168 during the initial stages of infection. We prepared samples from conidia (the inoculum) peeled epidermal cotyledon tissue susceptible...
Microbial community structures associated with halophytes and their compositions among different habitats, particularly natural saline sites, have not yet been investigated in detail. In the present study, we examined diversity composition of rhizosphere root endosphere bacteria two halophytes, Salicornia europaea L. Glaux maritima L., collected from adjacent brackish lakes, Lake Notoro Tofutsu, Japan. The bacterial species richness indices both lakes showed no significant differences or...
Abstract Next-generation sequencing technology may clarify microbiota that are as yet poorly understood in the soil, rhizosphere, and phyllosphere of vineyards. To provide new information on interaction between grapevine microorganisms, we focused endophytic grapevine. We performed microbiome analysis shoot xylems four cultivars, Vitis vinifera cvs. Chardonnay, Pinot Noir, Cabernet Sauvignon, sp. cv. Koshu, grown eleven vineyards Japan. The number fungal species was small could not be...
Salinity is a critical environmental factor that adversely affects crop productivity. Halophytes have evolved various mechanisms to adapt saline environments. Salicornia europaea L. one of the most salt-tolerant plant species. It does not special salt-secreting structures like salt gland or bladder, and therefore good model for studying common underlying tolerance. To identify candidate genes encoding key proteins in mediation tolerance S. europaea, we performed functional screen cDNA...
Biofertilizers containing high-density plant growth-promoting bacteria are gaining interest as a sustainable solution to environmental problems caused by eutrophication. However, owing the limitations of current investigative techniques, selected microorganisms not always preferred host plant, preventing recruitment into native microbiota or failing induce effects. To address this, five nitrogen-fixing previously isolated from water yam (Dioscorea alata L.) plants and showing dominant...
Fungal dimorphism involves two morphologies: a unicellular yeast cell and multicellular hyphal form. Invasion of hyphae into human cells causes severe opportunistic infections. The transition between forms is associated with the virulence fungi; however, mechanism poorly understood. Therefore, we aimed to identify factors that induce growth Trichosporon asahii, dimorphic basidiomycete trichosporonosis. T. asahii showed poor formed small containing large lipid droplets fragmented mitochondria...
The bacterial diversity and composition of water yam (Dioscorea alata L. cv. A-19), which can grow without chemical fertilization, have recently been characterized with no significant differences compared the use fertilization. However, community structure bacteria associated white Guinea rotundata), most cultivated economically important in West Africa, not yet investigated. This study bulk soil, rhizosphere, plant roots six genotypes (S004, S020, S032, S042, S058, S074) field experiments...