A5039696162- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Drug Transport and Resistance Mechanisms
- melanin and skin pigmentation
- Neuroscience of respiration and sleep
- Sphingolipid Metabolism and Signaling
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cholesterol and Lipid Metabolism
- Sleep and Wakefulness Research
- Nitric Oxide and Endothelin Effects
- Peptidase Inhibition and Analysis
- Trauma Management and Diagnosis
- Respiratory Support and Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- CRISPR and Genetic Engineering
- Biochemical effects in animals
- Entomopathogenic Microorganisms in Pest Control
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Heart Rate Variability and Autonomic Control
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Cardiac Structural Anomalies and Repair
Novartis Institutes for BioMedical Research
2007-2017
Novartis (Switzerland)
2007-2017
Centre National de la Recherche Scientifique
1999-2002
Institut de Pharmacologie Moléculaire et Cellulaire
1999-2002
École Supérieure de Biotechnologie de Strasbourg
1999
Instituto Butantan
1999
Blood gas and tissue pH regulation depend on the ability of brain to sense CO2 and/or H(+) alter breathing appropriately, a homeostatic process called central respiratory chemosensitivity. We show that selective expression proton-activated receptor GPR4 in chemosensory neurons mouse retrotrapezoid nucleus (RTN) is required for CO2-stimulated breathing. Genetic deletion disrupted acidosis-dependent activation RTN neurons, increased apnea frequency, blunted ventilatory responses CO2....
Pulmonary fibrosis can be experimentally induced in small rodents by bleomycin. The antibiotic is usually administered via the intratracheal or intranasal routes. In present study, we investigated oropharyngeal aspiration of bleomycin as an alternative route for induction lung rats and mice. development injury was followed vivo ultrashort echo time magnetic resonance imaging (UTE-MRI) post-mortem analyses (histology collagen, hydroxyproline determination, qRT-PCR). C57BL/6 mice, led to more...
BackgroundA novel family of proton-sensing G protein-coupled receptors, including OGR1, GPR4, and TDAG8, was identified to be important for physiological pH homeostasis inflammation. Thus, we determined the function OGR1 in intestinal mucosa.
The identification of a natural ligand the orphan chemoattractant receptor GPR15 provides mechanistic insight into migration lymphocytes in skin.
Using a genomics-based approach for screening orphan G-protein-coupled receptors, we have identified and cloned novel high-affinity, melanin-concentrating hormone (MCH) receptor. This receptor, named S643b, displays the greatest overall identity (32%) with previously reported human SLC-1 receptor (MCH1) to lesser extent somatostatin subtypes. The gene encoding S643b spans more than 23 kilobase pairs (kb) was mapped, by radiation hybrid experiments, on chromosome 6q14.3-q15. Comparison of...
Generation of gain-of-function transgenic mice by targeting the Rosa26 locus has been established as an alternative to classical produced pronuclear microinjection. However, transgenes endogenous promoter results in moderate ubiquitous expression and is not suitable for high levels. Therefore, we now generated a modified (modRosa26) that combines efficient targeted transgenesis using recombinase-mediated cassette exchange (RMCE) Flipase (Flp-RMCE) or Cre recombinase (Cre-RMCE) with transgene...
Shortened, more stable and weakly hydrophobic analogues of melanin-concentrating hormone (MCH) were searched as candidates for radioiodination. Starting from the dodecapeptide MCH(6 - 17), we found that: (1) substitution Tyr(13) by a Phe residue; (2) addition 3-iodo-Tyr residue at N-terminus; (3) hydrophilic spacer 8-amino-3,6-dioxyoctanoyl between 17) (compound S36057), led to an agonist potent than MCH itself in stimulating [35S]-GTPgammaS binding membranes HEK293 cells stably expressing...
Several studies have shown that melanin-concentrating hormone (MCH) is an orexigenic peptide in rat. In the present study, a structure-activity relationship with MCH analogs was performed rat, both vitro and vivo. On rat recombinant SLC-1 receptor, cAMP inhibition [(125)I]S36057 binding were measured. vivo, these injected intracerebroventricularly rats their effects evaluated upon food intake. First, data obtained receptor highly correlated those from its human counterpart. Second, agonist...
Background: The proton-activated G protein-coupled receptor GPR4 is expressed in many tissues including white adipose tissue. activated by extracellular protons the physiological pH range (i.e. 7.7 - 6.8) and coupled to production of cAMP. Methods: We examined mice lacking glucose tolerance insulin sensitivity young aged as well fed with a high fat diet. Expression profiles pro- anti-inflammatory cytokines tissue, liver skeletal muscle was assessed. Results: Here we show that have an...
Melanin-concentrating hormone (MCH) is a cyclic neuropeptide, with major role in stimulation of feeding behavior mammals. MCH signals the brain occur via two seven-transmembrane G protein-coupled receptors, namely MCH1 (SLC-1, MCH<sub>1</sub>, MCH-R1, or MCH-1R) and MCH2 (SLT, MCH<sub>2</sub>, MCH-R2, MCH-2R). In this study, we demonstrate that pro-MCH<sub>131–165</sub> peptide neuropeptide-glutamic acid-isoleucine (NEI)-MCH more potent than stimulating rat. Using rat MCH1-expressed human...
The proton-sensing G protein-coupled receptor GPR4 is expressed in different organs including the kidney and increases intracellular cAMP levels upon a decrease extracellular pH transfected cells. We found several nephron segments. examined phenotype of male KO mice, under control conditions dietary protocols. mice are smaller than their litter mates exhibit mild metabolic acidosis with higher anion gap, hypernatriemia hyperkaliemia. After 24 hrs challenge acid-base status was similar both...
Conference Abstract| October 01 1999 Optimisation of human mu opioid receptor expression in baculovirus-infected insect cells Massotte Dominique; Dominique #Departement des Récepteurs et Protéines membranaires, CNRS UPR 9050, Ecole Supérieure de Biotechnologie Strasbourg. F-67400 Illkirch-Graffenstaden, France$Institute Butantan, Laboratorio Imunologia Viral, 05503-900 Sao Paulo, Brasil Search for other works by this author on: This Site PubMed Google Scholar Baroche Laurence; Laurence...