A5043703885- Inflammatory Bowel Disease
- Hematopoietic Stem Cell Transplantation
- Immune Response and Inflammation
- Stress Responses and Cortisol
- Regulation of Appetite and Obesity
- Microscopic Colitis
- Gut microbiota and health
- Growth Hormone and Insulin-like Growth Factors
- Vestibular and auditory disorders
- Protein purification and stability
- Sphingolipid Metabolism and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Caveolin-1 and cellular processes
- Global Educational Reforms and Inequalities
- interferon and immune responses
- Immunotherapy and Immune Responses
- Reflective Practices in Education
- Biotin and Related Studies
- Eosinophilic Esophagitis
- Lipoproteins and Cardiovascular Health
- Higher Education Research Studies
- Immune cells in cancer
- Cellular transport and secretion
University of Cambridge
2024
PredictImmune
2024
MRC Epidemiology Unit
2024
Novartis Institutes for BioMedical Research
2012-2021
Novartis (Switzerland)
2010-2021
Novartis (United Kingdom)
2012
New York University
2007
University of California, San Francisco
1997
Uniformed Services University of the Health Sciences
1993
Naval Submarine Medical Research Laboratory
1993
Gonadotropes that secrete LH were demonstrated microscopically among mixed anterior pituitary (AP) cells in culture with a reverse hemolytic plaque assay. released from cell binds to adjacent RBCs bearing an Ab-protein A complex which results zone of complement-mediated hemolysis (a plaque) surrounding each gonadotrope. In untreated cultures few, small plaques formed, but the presence hypothalamic CnRH (10−7 M, maximally stimulating dose) 10-fold increase occurred their number and size....
The identification of a natural ligand the orphan chemoattractant receptor GPR15 provides mechanistic insight into migration lymphocytes in skin.
Cultured adenohypophysial cells secreting PRL were detected with a reverse hemolytic plaque assay. In this assay, secretion from pituitary cell results in hemolysis of cocultured protein A-coupled ovine erythrocytes the presence antiserum and complement, so that zone (a plaque) surrounds each lactotrope. The extent was related to amount secreted by lactotrope: batches cohort incubated under similar conditions either petri dishes for measurement RIA or Cunningham chambers area revealed...
The integration of host genetics, environmental triggers and the microbiota is a recognised factor in pathogenesis barrier function diseases such as IBD. In order to determine how these factors interact regulate immune response ecological succession colon tissue-associated microbiota, we investigated temporal interaction between following disruption colonic epithelial barrier.Oral administration DSS was applied mechanistic model damage resulting inflammation characterized for various...
Adenosine possesses potent anti‐inflammatory properties which are partly mediated by G i ‐coupled adenosine A3 receptors (A3Rs). A3R agonists have shown clinical benefit in a number of inflammatory conditions although some studies A3R‐deficient mice suggest pro‐inflammatory role. We hypothesised that, addition to cell signalling effects, compounds might inhibit neutrophil chemotaxis disrupting the purinergic feedback loop controlling leukocyte migration. Human activation triggered rapid...
IL-10 contributes to the maintenance of intestinal homeostasis via regulation inflammatory responses enteric bacteria. Loss signaling results in spontaneous colitis mice and early onset enterocolitis humans. Nucleotide-binding oligomerization domain (NOD) 2 is an intracellular receptor bacterial peptidoglycan products, and, although NOD2 mutations are associated with Crohn's disease, precise role development inflammation remains undefined. To determine on clinically relevant genetic...
Background A homeostatic relationship with the intestinal microflora is increasingly appreciated as essential for human health and wellbeing. Mutations in leucine-rich repeat (LRR) domain of Nod2, a bacterial recognition protein, are associated development inflammatory bowel disorder, Crohn's disease. We investigated molecular mechanisms underlying disruption symbiosis patients carrying Nod2 mutations. Methodology/Principal Findings In this study, using purified recombinant LRR domains, we...
The paracaspase Malt1 is a key regulator of canonical NF-κB activation downstream multiple receptors in both immune and nonimmune cells. Genetic disruption protease function mice MALT1 mutations humans results reduced regulatory T cells progressive multiorgan inflammatory pathology. In this study, we evaluated the altered homeostasis autoimmune disease protease-deficient (Malt1PD) Ags driving manifestations. Our data indicate that B cell IgG1/IgE production triggered by microbial dietary...
Light microscopic immunocytochemistry was utilized on plastic-embedded, acrolein-fixed pituitary glands from intact rats and killed 14 days after ovariectomy to determine which cell types were undergoing division. A significant increase in the number of divisions anterior cells seen ovariectomized compared controls. Most dividing immunocytochemically identified as gonadotrophs, but somatotrophs non-immunoreactive also found. All gonadotrophs stained with both anti-FSH beta anti-LH beta....
Journal Article Myopathy associated with HMG-CoA reductase inhibitors (HMGRIs) and cydosposin A: evaluation in a rat model Get access P. F. Smith, Smith Correspondence: PhD, Department of Safely Assessment, Merck Sharp & Dohme Research Laboratories (WP45-234), West Point, PA 19486, U.S.A. Search for other works by this author on: Oxford Academic PubMed Google Scholar R. S. Eydelloth, Eydelloth J. Grossman, Grossman Stubbs, Stubbs †Departments Safety Assessment Drug Metabolism,...
The quantitative relationship between receptor binding and hormone secretion at the single-cell level was investigated in present study by combining a reverse hemolytic plaque assay for measurement of luteinizing (LH) from individual pituitary cells with an autoradiographic 125I-labeled gonadotropin-releasing (GnRH) agonist to same cells. In assay, LH induces complement-mediated lysis LH-antibody-coated erythrocytes around gonadotropes, resulting areas (plaques). release gonadotropes...
High-risk hematologic cancers are potentially cured by allogeneic hematopoietic stem cell transplantation (alloHSCT).1 The success of this therapy is currently limited, however, the occurrence acute graft-versus-host disease (GVHD), an inflammatory process triggered alloreactive donor T-cells.2 classical target organs include skin, liver, and gastrointestinal tract. However, a prominent feature patients with GVHD also disruption regular posttransplantation T-cell immune recovery for which at...