Estimating the impacts of anthropogenic activities and climate change on species' spatial distributions is crucial for conservation. In this study, based 62 valid occurrence records Sphenomorphus incognitus 24 environmental factors (19 factors, 4 topographic 1 human activity factor), we utilized biomod2 combined model platform to predict suitable habitats S. under two current scenarios (Scenario 1: natural state; Scenario 2: interference state) future (SSP1-2.6 SSP5-8.5) in 2050s 2090s. The...

Crop yield is determined by the capacity of plant canopy to capture light energy and store nitrogen (Sinclair Sheehy, 1999), which in turn depends mostly on photosynthetic rate per se architecture plant. Leaf angle (LA), defined as inclination between leaf blade midrib vertical culm, one most important parameters describing (Anderson Denmead, 1969). It a complex trait, several related quantitative trait loci (QTLs) have been identified; however, only two these functionally characterized...

The identification of underlying differences is crucial for understanding the embryogenesis species specificity. In this paper, a comparative transcriptome analysis multi-species (CTAMS) was first developed to detect genome activation among human, mouse, and bovine. annotation intra-species detection inter-species difference were separately avoid potential batch effects. profile clusters Pearson correlation showed human bovine embryos displayed high similarity in with dramatic maternal...

Male and female Chinese soft-shelled turtles (Pelodiscus sinensis) have sex-dimorphic growth patterns, males higher commercial value because of their larger size thicker calipash. Thus, developing sex-specific markers is beneficial to studies on all-male breeding in P. sinensis. Here, we developed an accurate efficient workflow for the screening sequences with ZW or XY sex determination systems. Based this workflow, male sinensis reference genomes 2.23 Gb 2.26 were obtained using de novo...

Cronobacter spp. (Enterobacter sakazakii) is an important pathogen contaminating powdered infant formula (PIF). To describe the genotypic diversity of isolated in China, we identified isolates using fusA allele sequencing, and subtyped all pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), multiple-locus variable-number tandem-repeat analysis (MLVA). A total 105 were identified, which included C. sakazakii (58 isolates), malonaticus (30 dublinensis (11 turicensis (5...

We studied acute and joint toxicity of three different agrochemicals (chlorantraniliprole, flubendiamide-abamectin penoxsulam) to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles with the method stability water tests. Results showed that increased tadpole mortality. For toxicity, LC50 values after 24, 48 72 h chlorantraniliprole, penoxsulam exposure were 5.37, 4.90 4.68 mg/L; 0.035, 0.025 0.021 1.74, 1.45 1.29 mg/L, respectively. The safety concentrations (SC) 1.23, 0.30 0.003 Based on...

The Asian yellow pond turtle (Mauremys mutica) is an important commercial freshwater aquaculture species in China. This a highly sexually dimorphic species, with males growing at faster rate than females and exhibits temperature-dependent sex determination (TSD), which the incubation temperature during embryonic development determines sexual fate. However, mechanisms of or differentiation are remain mystery.

Hybrid weakness is a post-zygotic hybridization barrier frequently observed in plants, including rice. In this study, we describe the genomic variation among three temperate japonica rice (Oryza sativa ssp. japonica) varieties 'Aranghyangchalbyeo' ('CH7'), 'Sanghaehyangheolua' ('CH8') and 'Shinseonchalbyeo' ('CH9'), carrying different hybrid genes. The reciprocal progeny obtained from crossing any two displayed characteristic traits. We mapped cloned new locus, Hwc3 (hybrid 3), on chromosome...

We recorded and described the calls acoustic characteristics of male tiger frog, Hoplobatrachus rugulosus, in an artificial pond during mating season. Spectral temporal call parameters, along with intensity were analyzed. Three harmonics distinguishable from spectrogram. Four patterns dominant frequency found produced late at night, i.e, 3 first harmonic (located 500 Hz section, 700 800 section respectively) 1 second section). Call duration, duty cycle, intensity, pulse rate highly variable...

Though light conditions are known to affect the development and anti-predation strategies of several aquatic species, relatively little is about how different species react light, or can these during points in their life-cycle. In this study, we used four sympatric anuran tadpoles (Bufo gargarizans, B. melanostictus, Pelophylax nigromaculatus Microhyla fissipes) as animal system examine species-specific activities underdoing intensity treatments, so better understand they respond light. We...

Gonadal cell lines possess the abilities of self-renewal and differentiation, being used as an efficient tool to analyzing genes' functions involved in sex differentiation gametogenesis. Although some significant achievements have been obtained gonadal cells' culture or manipulation across multiple phyla including teleost mammals, there is limited study on turtles. In this study, we established a new ovarian line from young Asian yellow pond turtle (Mauremys mutica), which exhibited normal...

Both historical (e.g., glaciations) and spatial (geological features) factors are considered to have had significant influences on the distribution genetic structure of many plants. To understand effects such current geographical distributions plant species in Nanling central regions China, we examined variation phylogeographical patterns Sagittaria lichuanensis J.K. Chen (Alismataceae), an endangered endemic using chloroplast DNA atpB–rbcL intergenic spacer sequence variation. In this...

Abstract The DAZ family genes, including boule, dazl , and daz play pivotal roles in germ cell development differentiation during gametogenesis organisms, which have been widely studied mammals, reptiles, or fishes. Dazl was bisexual expressed both mitotic meiotic cells, male premeiotic expressed, whereas boule exhibits largely unisexual cells but expression several fishes, however, there is lack of report on gene the evolutionary conservation divergence reptile. Here, genes were...

The HLA‐B*13:20 allele differs from the closest matching B*13:01:01 by one nucleotide substitution in exon 3 at nt 527 (T→A).

We used Hoplobatrachus chinensis tadpoles as a model species to evaluate if the locomotor costs of tail loss could be compensated by regeneration. Different proportion (0%, 20%, 40% and 60%) segment were removed in four experimental groups reared for 7 days. Swimming speed was measured three times each tadpole: before removal, after days from removal. Gosner’s stage, body length, length survival rate tadpole removal at daily regenerative remaining 20% 60% treatments. Overall, our results...

<title>Abstract</title> Background The Asian yellow pond turtle (<italic>Mauremys mutica</italic>) is an important commercial freshwater aquaculture species in China. a highly sexual dimorphic species, with males growing at faster rate than females. exhibits temperature-dependent sex determination (TSD), which the incubation temperature during embryonic development determines fate. However, mechanisms of or differentiation are remain mystery. Results Here, we performed temperature-specific...

Asian warty newts, genus Paramesotriton , are endemic to southern China and northern Vietnam. Despite the achievements in biodiversity, molecular systematics biogeography of species this genus, population genetic diversity studies lacking due lack economical available markers. In study, we developed 17 highly polymorphic microsatellite loci from RAD simplified genomic data for successfully completed cross-species amplification tests on 20 samples four . These markers can be used as important...