A5103155033- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Particle accelerators and beam dynamics
- Plasma Diagnostics and Applications
- Atomic and Molecular Physics
- Atomic and Subatomic Physics Research
- Chemical Synthesis and Analysis
- Ion-surface interactions and analysis
- Magnetic confinement fusion research
- Microbial Natural Products and Biosynthesis
- Gyrotron and Vacuum Electronics Research
- Biochemical and Structural Characterization
- Lysosomal Storage Disorders Research
- Leprosy Research and Treatment
- Microbial Metabolic Engineering and Bioproduction
- Per- and polyfluoroalkyl substances research
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Atmospheric Ozone and Climate
- Antimicrobial Peptides and Activities
- Laser-induced spectroscopy and plasma
- Iron Metabolism and Disorders
- Microwave-Assisted Synthesis and Applications
Shimadzu (Japan)
2011-2024
Doshisha University
2004-2008
The University of Tokyo
1998
Abstract Lipidomics and metabolomics communities comprise various informatics tools; however, software programs handling multimodal mass spectrometry (MS) data with structural annotations guided by the Standards Initiative are limited. Here, we provide MS-DIAL 5 for in-depth lipidome elucidation through electron-activated dissociation (EAD)-based tandem MS determining their molecular localization imaging (MSI) using a species/tissue-specific database containing predicted collision-cross...
Gas-phase hydrogen radicals were introduced into a quadrupole ion trap containing singly charged phospholipids to obtain structural fragmentation patterns in tandem mass spectrometry (MS/MS). Saturated and unsaturated phosphatidylcholines used as model phospholipid, whose chain-length ranges between 16 24. The MS/MS spectrum yielded continuous series of fragment ions with difference 14 Da, representing the saturated fatty acyl chains. corresponding double-bond position within single chain...
Dissociation of gas-phase peptide ions through interaction with low-energy hydrogen (H) radical (∼0.15 eV) was observed a quadrupole ion trap mass spectrometry. The H generated by thermal dissociation H2 molecules passing heated tungsten capillary (∼2000 °C) injected into the containing target ions. fragmentation spectrum showed abundant c-/z- and a-/x-type ions, attributable to attachment/abstraction to/from ion. Because neutral initiated fragmentation, charge state precursor maintained...
Niemann-Pick disease type C (NPC) is an autosomal recessive disorder caused by the mutation of cholesterol-transporting proteins. In addition, early treatment important for good prognosis this because progressive neurodegeneration. However, diagnosis difficult due to a variety clinical spectrum. Lysosphingomyelin-509, which one most useful biomarkers NPC, was applied rapid and easy detection NPC. The fact that its chemical structure unknown until recently implicates unrevealed...
Mass spectrometry-based untargeted lipidomics has revealed the lipidome atlas of living organisms at molecular species level. Despite double bond (C = C) position being a crucial factor in biological system, C defined structures have not yet been characterized comprehensively. Here, we present an approach for position-resolved using combination oxygen attachment dissociation and computational mass spectrometry to increase annotation rate. We validated accuracy our platform as per authentic...
Tandem mass spectrometry (MS/MS) with radical-based fragmentation involving the attachment or abstraction of hydrogen to peptides, in a process called attachment/abstraction dissociation (HAD), has been recently developed. HAD-MS/MS is considered useful method for analysis proteins post-translational modification (PTM) because its ability determine PTM site on proteins. In present investigation, we analyzed highly acidic sulfopeptides and sulfoprotein digests using negative-ion combined...
Phosphorylation is the most abundant protein modification, and tandem mass spectrometry (MS/MS) with radical-based fragmentation techniques has proven to be a promising method for phosphoproteomic applications, owing its ability determine phosphorylation sites on proteins. The radical-induced technique involves attachment or abstraction of hydrogen peptides in an ion trap spectrometer, process called attachment/abstraction dissociation (HAD), which only been recently developed. In present...
Mass spectrometry with hydrogen-radical-mediated fragmentation techniques has been used for the sequencing of proteins/peptides. The two methods, matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) and hydrogen attachment/abstraction dissociation (HAD) are known as techniques. MALDI-ISD occurs during induced desorption processes, whereas HAD utilizes association peptide ions in gas phase. In this study, general mechanisms peptides were investigated. We demonstrated four...
In this study, we use a combination of tandem mass spectrometry and hydrogen radical-mediated fragmentation techniques to analyze the sequence peptides. We focus on induced by attachment atoms histidine tryptophan residue side-chains in peptide that occurs gas-phase. The atom attached imidazole indole rings residues, respectively, resulting intermediate experienced Cα-Cβ bond cleavage. detailed mechanism is investigated computational analysis using density functional theory. According...
Because both disulfide and peptide backbone bonds were cleaved by a single hydrogen attachment event, tandem mass spectrometry with dissociation allows the sequencing of peptides containing bonds.
Tandem mass spectrometry with fragmentation involving the reaction hydrogen atoms is expected to be useful for analysis of peptides and proteins. In general, preferentially react odd-electron radicals. The attachment even-electron peptide ions barely observed because their low rate. To date, only methodology developed by our group has successfully induced reacting atoms. present study, we focused on temperature in an ion trap spectrometer understand mechanism corresponding reaction. Because...
Ribosomally synthesized and posttranslationally modified peptides (RiPPs) with polar-functionalized fatty acyl groups are a rarely found untapped class of natural products. Although fatty-acylated RiPPs (PFARs) have potential as antimicrobial agents, the repertoire is still limited. Therefore, expanding chemical space expected to contribute development pharmaceutical agents. In this study, we performed genome mining stable isotope-guided comparative metabolomics discover new PFAR We focused...
A thermal cracking cell that served as the atomic hydrogen source for attachment/abstraction dissociation (HAD) analysis has an intrinsic problem to produce a beam of atoms reactive against heated tungsten capillary. plasma excited by 2.45 GHz microwave discharge can deliver species quadrupole ion trap confining analyte ions without excessive heating radical components. The (H•) production performance developed was evaluated optical emission spectroscopy and H• attachment reaction fullerene...
A compact magnetic field free atomic beam source was designed, assembled and tested the performance to produce hydrogen nitrogen atoms. forced air-cooled solid-state microwave power supply at 2.45 GHz frequency drives up 100 W through a coaxial transmission cable coupled triple stub tuner for realizing proper matching condition discharge load. The structure of affected range operation pressure, pressure reduced by four orders magnitude improving electrode geometry enhance local electric...
Lipids, a class of biomolecules, play significant role in the physiological system. In this study, gas-phase hydroxyl radicals (OH·) and atomic oxygens (O) were introduced into collision cell triple quadruple mass spectrometer (TQ-MS) to determine positions double bond unsaturated phospholipids. A microwave-driven compact plasma generator was used as OH·/O source. The reaction between precursor ions passing through generates product that correspond fatty acyl chain. This position specific...
Tandem mass spectrometry (MS/MS) with radical-based fragmentation was developed recently, which involves the reaction of hydrogen atoms and peptides in a process called attachment/abstraction dissociation (HAD). HAD mainly produces [cn + 2H]+ [zm via attachment to carbonyl oxygen on peptide backbone. In addition, often generates [an [xm 2H]+. To explain formation 2H]+, carbon atom backbone is proposed initiate Cα–C bond cleavage. The resultant hydrogen-abundant oxygen-centered radical...
The transport velocity of a plasma containing photodetached electrons was measured by injecting semiconductor laser at power density 8 W/cm2 into the center linearly magnetized cylindrical plasma. hydrogen produced with pair coils and ring shaped array discharge filaments contained H− about 3% electron density. For gas pressure 1 Pa, perturbed decreased from 2500 to 400 m/s as linear magnetic field increased 1.5 15 mT. became one half original value when 2 Pa. observed agreed predicted...
Time evolution of photodetachment perturbation signal induced by a diode laser was observed in an O2 plasma. Photodetachment current collected Langmuir probe directly measured and recorded digital oscilloscope. After integrating the data, waveform showed time dependence resembling error function. The had changed its shape accordance as position between beam axis changed. These characteristics are well explained diffusion model. method has possibility to yield information on various negative...
A molybdenum plasma grid was installed in a 30-cm-long 16-cm-diam 16-pole magnetic multicusp ion source to simulate the effect of electrode bias negative source. Effects caused by voltage applied upon parameters, density hydrogen ions (H−), and drift velocity perturbed photodetachment H− were investigated with direct current laser method. The electron at distance 0.5 cm from decreased 8×1010 4×1010cm−3 increasing 0 +4V, while increased 8×108 4.5×109cm−3. perturbation changed factor 3...
Performances of microwave driven compact neutral beam sources different plasma excitation configurations have been compared in producing directed low energy atomic beams for biomolecular structural analyses. The include unique impedance matching systems themselves reducing the size source; a multi-turn spiral antenna successfully excited hydrogen 4 mm inner diameter Al2O3 tube by 2.45 GHz power. A combination mirror magnetic field and electron cyclotron resonance has found effective to...
Abstract Oxygen attachment dissociation (OAD) is a tandem mass spectrometry (MS/MS) technique used to annotate the positions of double bonds (C=C) in complex lipids. Although OAD has been for untargeted lipidomics, its availability limited positive-ion mode, requiring independent use collision-induced (CID) method. In this study, we demonstrated OAD-MS/MS negative-ion mode profiling phosphatidylserines, phosphatidylglycerols, phosphatidylinositols, and sulfatides, where fragmentation...
Polyfluoroalkyl substances (PFAS) like perfluorooctanoic acid have persistent environmental and physiological effects. This study investigates the degradation of C