A5065419784- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Diatoms and Algae Research
- Microfluidic and Bio-sensing Technologies
- Geomagnetism and Paleomagnetism Studies
- Cancer Cells and Metastasis
- Protist diversity and phylogeny
- Biosensors and Analytical Detection
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Lipid metabolism and biosynthesis
- Cell Image Analysis Techniques
- Genomics and Phylogenetic Studies
- Microfluidic and Capillary Electrophoresis Applications
- Glycosylation and Glycoproteins Research
- Biodiesel Production and Applications
- Characterization and Applications of Magnetic Nanoparticles
- Advanced Proteomics Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Magnetic and Electromagnetic Effects
- Cancer Research and Treatments
- Image Processing Techniques and Applications
Tokyo University of Agriculture and Technology
2015-2024
Life Science Institute
2015-2024
Institute of Engineering
2012-2018
Google (United States)
2017
Japan Science and Technology Agency
2013-2015
Hokkaido University
2012
Shizuoka Cancer Center
2009
Tohoku University
2007
Waseda University
2005-2006
University of California, Irvine
2002
Circulating tumor cells (CTCs) are circulating in the peripheral blood of patients with metastatic cancer. Detection CTCs has clinical significance cancer therapy because it would enable earlier diagnosis metastasis. In this research, a microfluidic device equipped size-selective microcavity array for highly efficient and rapid detection from whole was developed. The can specifically separate on basis differences size deformability between hematologic cells. Furthermore, recovered were...
Oleaginous photosynthetic organisms such as microalgae are promising sources for biofuel production through the generation of carbon-neutral sustainable energy. However, metabolic mechanisms driving high-rate lipid in these oleaginous remain unclear, thus impeding efforts to improve productivity genetic modifications. We analyzed genome and transcriptome diatom Fistulifera solaris JPCC DA0580. Next-generation sequencing technology provided evidence an allodiploid structure, suggesting...
Background Epithelial cell adhesion molecule (EpCAM)-based enumeration of circulating tumor cells (CTC) has prognostic value in patients with solid tumors, such as advanced breast, colon, and prostate cancer. However, poor sensitivity been reported for non-small lung cancer (NSCLC). To address this problem, we developed a microcavity array (MCA) system integrated miniaturized device CTC isolation without relying on EpCAM expression. Here, report the results clinical study CTCs which compared...
In this study, we present a method for efficient enrichment of small-sized circulating tumor cells (CTCs) such as those found in the blood small-cell lung cancer (SCLC) patients using microcavity array (MCA) system. To enrich CTCs from whole blood, microfabricated nickel filter with rectangular MCA (10(4) cavities/filter) was integrated miniaturized device, allowing isolation based on differences size and deformability between cells. The shape porosity were optimized to efficiently capture...
Abstract Transcriptome analysis of circulating tumor cells (CTCs), which migrate into blood vessels from primary tissues, at the single-cell level offers critical insights biology metastasis and contributes to drug discovery. However, transcriptome single CTCs has only been reported for a limited number cancer types, such as multiple myeloma, breast, hepatocellular, prostate cancer. Herein, we report gastric single-CTCs. We utilized an antigen-independent strategy CTC isolation metastatic...
ABSTRACT Lipidomics and metabolomics communities comprise various informatics tools; however, software programs that can handle multimodal mass spectrometry (MS) data with structural annotations guided by the Standards Initiative are limited. Here, we provide MS-DIAL 5 to facilitate in-depth elucidation of lipids through electron-activated dissociation (EAD)-based tandem MS, as well determine their molecular localization MS imaging (MSI) using a species/tissue-specific lipidome database...
Abstract Lipidomics and metabolomics communities comprise various informatics tools; however, software programs handling multimodal mass spectrometry (MS) data with structural annotations guided by the Standards Initiative are limited. Here, we provide MS-DIAL 5 for in-depth lipidome elucidation through electron-activated dissociation (EAD)-based tandem MS determining their molecular localization imaging (MSI) using a species/tissue-specific database containing predicted collision-cross...
ABSTRACT Magnetic particles are increasingly used for various biomedical applications because they easy to handle and separate from biological samples. In this work, a novel anchor molecule was targeted protein display onto magnetic nanoparticles. The bacterium Magnetospirillum magneticum AMB-1 synthesizes intracellular bacterial (BMPs) covered with lipid bilayer membrane. our recent research, an integral BMP membrane protein, Mms13, isolated as functional proteins BMPs. anchoring properties...
Abstract Magnetic nanoparticles produced by magnetotactic bacterium, bacterial magnetic particles (BacMPs), covered with a lipid bilayer membrane (magnetosome membrane) can be used to separate specific target cells from heterogeneous mixtures because they are easily manipulated magnets and it is easy display functional proteins on their surface via genetic engineering. Despite possessing unique valuable characteristics, the potential toxicity of BacMPs separated has not been characterized in...
Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize production. This strategy would reduce costs through glycerol recycling and improve lipid productivity biomass by overcoming growth retardation caused decreased light penetration in high-density culture.Overexpression endogenous kinase (GK) gene an oleaginous marine diatom, Fistulifera solaris JPCC DA0580,...
For biodiesel production from microalgae, it is desirable to understand the entire triacylglycerol (TAG) metabolism. TAG accumulation occurs in oil bodies, and although body-associated proteins could play important roles metabolism, only a few microalgal species have been studied by comprehensive analysis. Diatoms are microalgae that promising producers of biodiesel, on which such proteomics analysis has not conducted date. Herein, we identified oleaginous diatom Fistulifera sp. strain JPCC...
Biofuel production using microalgae is believed to have the advantage of continuous year-round over crop plants, which strong seasonality. However, actual microalgal lipids outdoor mass cultivation has rarely been demonstrated. In our previous study, it was demonstrated that oleaginous diatom, Fistulifera solaris, culturable in bioreactors from spring autumn, whereas biomass and lipid winter failed because F. solaris did not grow below 15 °C. Therefore, another candidate strain required....
Here, we report the fabrication of a chemical gradient microfluidic device for single-cell cytotoxicity assays. This consists generator and microcavity array that enables entrapment cells with high efficiency at 88 ± 6% loaded cells. A 2-fold logarithmic is capable generating serial was designed then integrated array. High density demonstrated in without cell damage, which performed 30 s. Finally, validated feasibility this to perform assays by exposing potassium cyanide (0-100 μM KCN). The...
The immobilization of multiple cellulase complexes receiving attention for use in the efficient hydrolysis celluloses. In this study, magnetosome display system was employed preparation systems mimicking natural (cellulosomes) on magnetic nanoparticles (MNPs). Initially, two fluorescent proteins, namely, green protein and mCherry, were immobilized MNPs. Fluorescence analysis revealed close proximity different proteins Enzyme-linked immunosorbent assay showed that stoichiometrically...
A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach understanding their spatiotemporal dynamics living cells. However, the lack of adequate probes has hampered quantitative determination chemical composition and metabolomics cellular lipids at single-cell resolution. Here, we describe a method rapid, direct, lipid molecules from cells using Raman imaging. In localization form triacylglycerol (TAG) within oleaginous microalga molecular...
Summary F istulifera sp. strain JPCC DA0580 , a marine pennate diatom, contains extremely high levels of intracellular triglyceride and has been suggested as promising source feedstock for biodiesel fuels. was isolated from mangrove swamp located in S umiyo B ay below the mouths Y akugachi R ivers A mami‐ O hshima, K agoshima, J apan. In this study, morphology 18S rDNA sequence were compared with those other strains. possesses morphological characters genus namely lightly silicified...