A5066675786- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Mycotoxins in Agriculture and Food
- Metal-Organic Frameworks: Synthesis and Applications
- Potato Plant Research
- Identification and Quantification in Food
- Food Quality and Safety Studies
- Analytical Chemistry and Chromatography
- Carcinogens and Genotoxicity Assessment
- CRISPR and Genetic Engineering
- Advanced oxidation water treatment
- Crystal Structures and Properties
- Retinoids in leukemia and cellular processes
- Electron Spin Resonance Studies
- melanin and skin pigmentation
- Advanced Nanomaterials in Catalysis
- Biochemical Analysis and Sensing Techniques
- Atherosclerosis and Cardiovascular Diseases
- Research in Cotton Cultivation
- Covalent Organic Framework Applications
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Ziziphus Jujuba Studies and Applications
- Antioxidant Activity and Oxidative Stress
Linyi University
2022-2025
University of Chinese Academy of Sciences
2019-2024
Shanghai Institute of Materia Medica
2024
Chinese Academy of Sciences
2019-2024
Chinese Academy of Tropical Agricultural Sciences
2023
South Subtropical Crops Research Institute
2023
Research Center for Eco-Environmental Sciences
2019-2022
Qingdao University of Science and Technology
2007-2021
Guilin University of Technology
2020
Changchun Institute of Applied Chemistry
2020
We report an improved tandem mass spectrometric assay for retinoic acid (RA) applicable to in vitro and vivo biological samples. This liquid chromatography (LC/MS/MS) direct RA quantification is the most sensitive date, with a 62.5 attomol lower limit of detection linear range spanning greater than 4 orders magnitude (from 250 10 pmol). resolves all-trans-RA (atRA) from its endogenous geometric isomers, samples limited size (10−20 mg tissue), functions complex matrixes. Coefficients...
Aflatoxin B1 (AFB1), one of the most toxic mycotoxins, poses great health risks. Rapid and sensitive detection AFB1 is important for food safety, environment monitoring, risk assessment. We report here development a simple reusable electrochemical aptasensor rapid AFB1. Main improvements were achieved through engineering an aptamer containing short stem-loop structure enhancing binding affinity at lower temperature. The DNA with methylene blue (MB) label end was immobilized on gold...
We describe a simple aptamer assay for the rapid analysis of aflatoxin B1 (AFB1), potently carcinogenic fungal metabolite. The original anti-AFB1 was rationally truncated, obtaining short that displayed better performance. Different concentrations AFB1 were detected by measuring circular dichroism spectra this aptamer. detection limit reached 0.6 nM, which lower than (80 nM).
Electrochemical detection of AFB1 with square wave voltammetry by using a complementary DNA modified gold electrode and methylene blue labeled aptamer.
Aflatoxin B1 (AFB1) is one of the mycotoxins produced by Aspergillus flavus and parasiticus, it causes contamination in foods great risk to human health. Simple sensitive detection AFB1 important demanded for food safety quality control. Aptamers can specifically bind targets with high affinity, showing advantages affinity assays biosensors. We reported an aptamer structure-switch fluorescent aflatoxin (AFB1), using a label-free aptamer, fluorescein (FAM)-labeled complementary strand (FDNA),...
This paper reported an improved molecular beacon method for the rapid detection of aflatoxin B1 (AFB1), a natural mycotoxin with severe carcinogenicity. With assistance complementary DNA (cDNA) chain, which consists aptamer flanked by FAM and BHQ1 displayed larger fluorescent response to AFB1, contributing sensitive AFB1. Upon optimization some key experimental factors, AFB1 ranging from 1 nM 3 μM, within 20 min, was realized using this method. A limit (LoD) obtained, lower than LoD (8 nM)...
The DNA-encoded library (DEL) is a powerful hit generation tool for chemical biology and drug discovery; however, the optimization of DEL hits remained daunting challenge medicinal chemistry community. In this study, compounds targeting WIN binding domain WDR5 were discovered by initial three-cycle linear selection, their potency was further enhanced cascade selection from focused designed based on original first run hits. As expected, these new second more potent inhibitors in protein assay...