A5006320084- Metal complexes synthesis and properties
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Metal-Catalyzed Oxygenation Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Drug Transport and Resistance Mechanisms
- Peptidase Inhibition and Analysis
- Muscle Physiology and Disorders
- Advanced biosensing and bioanalysis techniques
- Coordination Chemistry and Organometallics
- Molecular Sensors and Ion Detection
- Asymmetric Hydrogenation and Catalysis
- Pesticide and Herbicide Environmental Studies
- Ferrocene Chemistry and Applications
- Trace Elements in Health
- Synthesis and Reactivity of Heterocycles
- Biosensors and Analytical Detection
- Asymmetric Synthesis and Catalysis
- Enzyme Catalysis and Immobilization
- Electrochemical Analysis and Applications
- Synthesis and Biological Evaluation
- Zebrafish Biomedical Research Applications
- Chemical Synthesis and Analysis
Bucknell University
2019-2025
University of Rochester
2024
University of York
2023
University of Maryland, College Park
2023
University of Toronto
1985-2021
Hospital for Sick Children
2015-2021
University of Calgary
2021
Genome Canada
2021
University of California, San Diego
2013-2018
University of California System
2016
Substitution of one pyridine by pyrazine in each DPA appendage ZP1 leads to a new zinc sensor, ZPP1, with modified background fluorescence and affinity. ZPP1 exhibits two-step response during titrations, which method for quantification. The ability image quantify was demonstrated pancreatic Min6 cells.
Abstract This microreview describes the structures and properties of a number bimetallic complexes designed as both structural functional mimics active sites some specific metallohydrolase enzymes. The metalloenzymes in question include predominantly monoesterase‐activity‐displaying purple acid phosphatase (PAP) di‐ triesterase enzymes, which have significant roles bioremedial hydrolysis organophosphate pesticides nerve gases. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Due to their structural and mechanical properties, 1D helical protein assemblies represent highly attractive design targets for biomolecular engineering design. Here we present a designed, tetrameric building block, Zn8R4, which assembles via Zn coordination interactions into series of crystalline, nanotubes whose widths can be controlled by solution conditions. X-ray crystallography transmission electron microscopy (TEM) measurements indicate that all classes are constructed through the...
The spread of antibiotic-resistant bacteria poses a global threat to public health. Conventional bacterial detection and identification methods often require pre-enrichment and/or sample preprocessing purification steps that can prolong diagnosis by days. Methicillin-resistant Staphylococcus aureus (MRSA) is one the most widespread leading cause hospital-acquired infections. Here, we have developed method specifically capture detect MRSA directly from patient nasal swabs with no prior...
As antibiotic-resistant bacteria spread worldwide, the need to develop novel antimicrobial agents is urgent. One rich source of potential antimicrobials insect immune system, as insects produce a wide range peptides (AMPs) with diverse sequences and structures. Insects also encounter many bacterial pathogens, some which are closely related pathogens clinical relevance. However, despite interest in AMPs therapeutics, relationships between amino acid sequence, biophysical properties, activity,...
We report the characterization of tris(2-pyridylmethyl)amine (TPA) as a membrane-permeable zinc chelator for intercepting biological mobile zinc. Compared to N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), TPA chelates with faster kinetics in cuvettes, live cells, and brain slices. also is generally less toxic than TPEN cell culture. Mechanistic analysis indicates that these improvements arise from both electronic steric properties including weaker metal-binding affinity, lower...
No AccessJournal of Urology1 Apr 1985Echinococcal Disease the Kidney Presenting as a Renal Filling Defect R.J. Buckley, S. Smith, Herschorn, R.H. Comisarow, and M. Barkin BuckleyR.J. Buckley More articles by this author , SmithS. Smith HerschornS. Herschorn ComisarowR.H. Comisarow BarkinM. View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)49134-8AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail We report...
A mixed-valence complex, [FeIIIFeIIL1(μ-OAc)2]BF4·H2O, where the ligand H2L1 = 2-{[[3-[((bis(pyridin-2-ylmethyl)amino)methyl)-2-hydroxy-5-methylbenzyl](pyridin-2-ylmethyl)amino]methyl]phenol}, has been studied with a range of techniques, and, possible, its properties have compared to those corresponding enzyme system purple acid phosphatase. The FeIIIFeII and FeIII2 oxidized species were spectroelectrochemically. temperature-dependent population S 3/2 spin states heterovalent system,...
Programmed cell death is essential for plant development and stress adaptation. A detailed understanding of the signal transduction pathways that regulate programmed requires identification underpinning protein networks. Here, we have used a protagonist antagonist triggered by fumonisin B1 as probes to identify key regulatory proteins in Arabidopsis. Our hypothesis was changes abundance death-regulatory induced should be blocked or attenuated concurrent treatment with antagonist. We focused...
Branched chain fatty acids are substrates for cytochrome P450(BM3) (CYP102) from Bacillus megaterium; oxidation of C15 and C17 iso anteiso by leads to the formation hydroxylated products that possess high levels regiochemical stereochemical purity.
The co-self-assembly of proteins and nucleic acids (NAs) produces complex biomolecular machines (e.g., ribosomes telomerases) that represent some the most daunting targets for design. Despite significant advances in protein DNA or RNA nanotechnology, construction artificial nucleoprotein complexes has largely been limited to cases rely on NA-mediated spatial organization units, rather than a cooperative interplay between protein- interactions typify natural assemblies. We report here...
A structural model for the active site of phosphoesterases, enzymes that degrade organophosphate neurotoxins, has been synthesised. The ligand [2-((2-hydroxy-3-(((2-hydroxyethyl)(pyridin-2-ylmethyl)amino)methyl)-5-methylbenzyl)(pyridin-2-ylmethyl)amino)acetic acid] (H3L1) and two Zn(II) complexes have prepared characterised as [Zn2(HL1)(CH3COO)](PF6)·H2O Li[Zn2(HL1)]4(PO4)2(PF6)3·(CH3OH). complex were through 1H NMR, 13C mass spectroscopy microanalysis. X-ray crystal structure...
The ligand, 2-((2-hydroxy-5-methyl-3-((pyridin-2-ylmethylamino)methyl)benzyl)(2-hydroxybenzyl)amino)acetic acid (H(3)HPBA), which contains a donor atom set that mimics of the active site purple phosphatase is described. Reaction H(3)HPBA with iron(III) or iron(II) salts results in formation tetranuclear complex, [Fe(4)(HPBA)(2)(OAc)(2)(mu-O)(mu-OH)(OH(2))(2)]ClO(4) x 5H(2)O. X-Ray structural analysis reveals cation consists four ions, two HPBA(3-) ligands, bridging acetate oxide ion and...
The binuclear heterovalent manganese model complex [Mn(II)Mn(III)(L1)(OAc)(2)] ClO(4) x H(2)O (H(2)L1 = 2-(((3-((bis(pyridin-2-ylmethyl)amino)methyl)-2-hydroxy-5-methylbenzyl)(pyridin-2-ylmethyl)amino)-methyl)phenol) has been prepared and studied structurally, spectroscopically, computationally. magnetic electronic properties of the have related to its structure. is weakly antiferromagnetically coupled (J approximately -5 cm(-1), H -2J S(1) S(2)) electron paramagnetic resonance (EPR)...
Many protein–protein interactions that play a central role in cellular processes involve α-helical domains. Consequently, there has been great interest developing strategies for stabilizing short peptides conformations toward the inhibition and interrogation of interactions. Here, we show tridentate hybrid coordination motifs (HCMs), which consist natural (histidine, His) an unnatural (8-hydroxyquinoline, Quin) metal binding functionality, can bind divalent ions with high affinity thereby...
The zebrafish model is an emerging system for the study of neuromuscular disorders. In diseases, integrity muscle membrane a critical disease determinant. To date, numerous conditions display degenerating fibers with abnormal integrity; this most commonly observed in muscular dystrophies. Evans Blue Dye (EBD) vital, cell permeable dye that rapidly taken into degenerating, damaged, or apoptotic cells; contrast, it not up by cells intact membrane. EBD injection employed to ascertain mouse...