A5045980922- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pluripotent Stem Cells Research
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Pharmacogenetics and Drug Metabolism
- Liver physiology and pathology
- Neuroscience and Neural Engineering
- Advanced Biosensing Techniques and Applications
- Anesthesia and Neurotoxicity Research
- Microfluidic and Bio-sensing Technologies
- Nanocomposite Films for Food Packaging
- Microfluidic and Capillary Electrophoresis Applications
- Tissue Engineering and Regenerative Medicine
- Animal testing and alternatives
- Adsorption and biosorption for pollutant removal
- Enzyme Catalysis and Immobilization
- Image Processing Techniques and Applications
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- Advanced Fluorescence Microscopy Techniques
- Glycosylation and Glycoproteins Research
- Surfactants and Colloidal Systems
- Drug Transport and Resistance Mechanisms
University of North Texas
2021-2025
University of North Texas at Dallas
2024
Cleveland State University
2013-2022
Cleveland Research (United States)
2018
Solidus Biosciences (United States)
2006-2012
Rensselaer Polytechnic Institute
2002-2009
Institute for Biotechnology and Bioengineering
2008
University of California, Berkeley
2005
Tokyo Institute of Technology
2001-2005
Korea Advanced Institute of Science and Technology
1997-1999
We have developed a miniaturized 3D cell-culture array (the Data Analysis Toxicology Assay Chip or DataChip) for high-throughput toxicity screening of drug candidates and their cytochrome P450-generated metabolites. The DataChip consists human cells encapsulated in collagen alginate gels (as small as 20 nl) arrayed on functionalized glass slide spatially addressable against multiple compounds. A single containing 1,080 individual cell cultures, used conjunction with the complementary...
We developed microarray 3D bioprinting technology and associated pillar/deep well plates that allow high-throughput, miniature, uniform culture of human liver organoids (HLOs) from bioprinted foregut cells facilitate in situ assessments.
Contemporary cancer therapy refers to treatment based on genetic abnormalities found in patient's tumor. However, this approach is faced with numerous challenges, including tumor heterogeneity and molecular evolution, insufficient samples available along information linking clinical outcomes, lack of therapeutic drugs containing pharmacogenomic information, technical limitations rapid drug efficacy tests quantities primary cells from patients. To address these problems improve outcomes...
Static three-dimensional (3D) cell culture has been demonstrated in ultralow attachment well plates, hanging droplet and microtiter plates with hydrogels or magnetic nanoparticles. Although it is simple, reproducible, relatively inexpensive, thus potentially used for high-throughput screening, statically cultured 3D cells often suffer from a necrotic core due to limited nutrient oxygen diffusion waste removal have vivo-like tissue structure. Here, we overcome these challenges by developing...
Abstract Human induced pluripotent stem cell (iPSC)-derived brain organoids have potential to recapitulate the earliest stages of development, serving as an effective in vitro model for studying both normal development and disorders. However, current organoid culture methods face several challenges, including low throughput, high variability generation, time-consuming, multiple transfer encapsulation cells hydrogels throughout culture. These limitations hinder widespread application...
The clinical progression of new chemical entities to pharmaceuticals remains hindered by the relatively slow pace technology development in toxicology and safety evaluation, particularly vitro approaches, that can be used preclinical early phases drug development. To alleviate this bottle-neck, we have developed a metabolizing enzyme assay chip (MetaChip) combines high-throughput P450 catalysis with cell-based screening on microscale platform. MetaChip concept is demonstrated using sol-gel...
We have developed a novel three-dimensional (3D) cellular microarray platform to enable the rapid and efficient tracking of stem cell fate quantification specific markers. This consists miniaturized 3D culture array on functionalized glass slide for spatially addressable high-throughput screening. A spotter was used deposit cells onto modified surface yield an consisting encapsulated in alginate gel spots with volumes as low 60 nL. method based immunofluorescence technique scaled down...
Abstract Human organoids have the potential to revolutionize in vitro disease modeling by providing multicellular architecture and function that are similar those vivo. This innovative evolving technology, however, still suffers from assay throughput reproducibility enable high‐throughput screening (HTS) of compounds due cumbersome organoid differentiation processes difficulty scale‐up quality control. Using for HTS is further challenged lack easy‐to‐use fluidic systems compatible with...
Oleic acid, found in a variety of food sources, is beneficial to health. However, studies on its anticancer effects cancer stem cells resistant conventional treatments and the mechanisms underlying such are scarce. Here, we evaluated oleic acid against breast with stemness characteristics. inhibited proliferation stem-like more significantly than that their respective parental cells, reduced expression cell markers migration invasion propensity. It also repressed phosphorylation FAK, AKT,...
Three-dimensional (3D) cellular assays closely mimic the in vivo milieu, providing a rapid, inexpensive system for screening drug candidates toxicity or efficacy early stages of discovery. However, 3D culture systems may suffer from mass transfer limitations, particularly delivery large polypeptide nucleic acid compounds. Nucleic acids (e.g., genes, silencing RNA) are particular interest both as potential therapeutics and due to desire modulate gene-expression patterns cells exposed...
ABSTRACT Recent advancements in three‐dimensional (3D) cell culture technologies, such as spheroids, organoids, and 3D bioprinted tissue constructs, have significantly improved the physiological relevance of vitro models. These models better mimic structure function, closely emulating vivo characteristics enhancing phenotypic analysis, critical for basic research drug screening personalized cancer therapy. Despite their potential, current platforms face technical challenges, which include...
We have developed an immunofluorescence-based assay for high-throughput analysis of target proteins on a three-dimensional cellular microarray platform. This process integrates the use microarrays, which should better mimic microenvironment, with sensitive immunofluorescence detection and provides quantitative information cell function. To demonstrate this platform, we examined accumulation alpha subunit hypoxia-inducible factor (HIF-1alpha) after chemical stimulation human pancreatic tumor...
Three-dimensional (3D) cancer cell culture models mimic the complex 3D organization and microenvironment of human solid tumor tissue are thus considered as highly predictive representing avascular regions.
Abstract Despite the potential toxicity of commercial chemicals to development nervous system (known as developmental neurotoxicity or DNT), conventional in vitro cell models have primarily been employed for assessment acute neuronal toxicity. On other hand, animal used DNT are not physiologically relevant due heterogenic difference between humans and animals. In addition, low-throughput, time-consuming, expensive, ethically questionable. Recently, human brain organoids emerged a promising...
Abstract Human liver organoids (HLOs) differentiated from embryonic stem cells (ESCs), induced pluripotent (iPSCs), and adult (ASCs) can recapitulate the structure function of human fetal tissues, thus being considered as a promising tissue model for diseases predictive compound screening. However, adoption HLOs in drug discovery faces several technical challenges, which include lengthy differentiation process with multiple culture media leading to batch-to-batch variation, short-term...