A5033043835- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Microbial Inactivation Methods
- Protease and Inhibitor Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Connective tissue disorders research
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- Insect and Arachnid Ecology and Behavior
- Cancer-related gene regulation
- Microfluidic and Bio-sensing Technologies
- Machine Learning in Bioinformatics
- RNA modifications and cancer
- Transgenic Plants and Applications
- Neurobiology and Insect Physiology Research
- Plant Genetic and Mutation Studies
- Peptidase Inhibition and Analysis
- Plant tissue culture and regeneration
Université Toulouse III - Paul Sabatier
2018-2025
Centre National de la Recherche Scientifique
2013-2025
Université de Toulouse
2018-2024
Laboratoire de Recherche en Sciences Végétales
2021-2022
Centre de Biologie du Développement
2018-2020
Institut de Pharmacologie et de Biologie Structurale
2013-2020
Adolescent cancer survivors present increased risks of developing secondary malignancies due to therapy. Electrochemotherapy is a promising anti-cancer approach that potentiates the cytotoxic effect drugs by application external electric field pulses. Clinicians proposed associate electroporation and calcium. The current study aims unravel toxic mechanisms calcium electroporation, in particular if presents genotoxic profile its cytotoxicity comes from ion itself or osmotic stress. Human...
Gene electrotransfection using micro- or millisecond electric pulses is a well-established method for safe gene transfer. For efficient transfection, plasmid DNA has to reach the nucleus. Shorter, high-intensity nanosecond (nsEPs) affect internal cell membranes and may contribute an increased uptake of by In our study, nsEPs were applied Chinese hamster ovary (CHO) cells after classical electrotransfer, with coding green fluorescent protein (GFP). Time gaps between electrotransfer varied...
ABSTRACT Matrix metalloproteinases have a broad spectrum of substrates ranging from extracellular matrix components and adhesion molecules to chemokines growth factors. Despite being mostly secreted, MMPs been detected in the cytosol, mitochondria or nucleus. Although most attention is focused on their role remodeling, diversity complex trafficking open possibility for non-canonical functions. Yet vivo examples experimental demonstration physiological relevance such activities are rare....
Abstract Background Recent genome-wide studies of many species reveal the existence a myriad RNAs differing in size, coding potential and function. Among these are long non-coding RNAs, some them producing functional small peptides via translation short ORFs. It now appears that any kind RNA presumably has to encode peptides. Accordingly, our team recently discovered plant primary transcripts microRNAs ( pri-miRs ) produce regulatory (miPEPs) involved auto-regulatory feedback loops enhancing...
Recent studies have shown that hundreds of small proteins were occulted when protein-coding genes annotated. These proteins, called alternative failed to be annotated notably due the short length their open reading frame (less than 100 codons) or enforced rule establishing messenger RNAs (mRNAs) are monocistronic. Several biologically active molecules and seem involved in a wide range biological functions. However, genome-wide exploration proteome is still limited few species. In present...
MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in nucleus by Microprocessor complex generating precursors (pre-miRNAs) that then exported to cytoplasm and processed into mature miRNAs. Some miRNAs hosted pri-miRNAs annotated as RNAs (lncRNAs) defined MIRHGs (for miRNA Host Genes). However, several lnc contain translatable open reading frames (smORFs). If smORFs present within lncRNAs can encode functional...
To achieve a highly differentiated state, cells undergo multiple transcriptional processes whose coordination and timing are not well understood. In Drosophila embryonic epidermal cells, polished-rice (Pri) smORF peptides act as temporal mediators of ecdysone to activate program leading cell shape remodeling. Here, we show that the ecdysone/Pri axis concomitantly represses transcription large subset cuticle genes ensure proper differentiation insect exoskeleton. The repression relies on...
Summary The transmembrane Matrix Metalloproteinase MMP14/MT1-MMP is known to promote cell migration by cleavage of the extracellular matrix. To initiate migration, epithelial cells need gain mesenchymal attributes. They reduce cell-cell junctions and apicobasal polarity migratory capabilities. This process named epithelial-mesenchymal transition (EMT). MMP14’s implication in EMT still ill-defined. We used chick neural crest (NC) as a model explore function MMP14 physiological EMT. Our...
Summary Background Recent genome-wide studies of many species reveal the existence a myriad RNAs differing in size, coding potential and function. Among these are long non-coding RNAs, some them producing functional small peptides via translation short ORFs. It now appears that any kind RNA presumably has to encode peptides. Accordingly, our team recently discovered plant primary transcripts microRNAs ( pri-miRNAs ) produce regulatory (miPEPs) involved auto-regulatory feedback loops...
Abstract Recent studies have shown that hundreds of small proteins were occulted when protein coding genes annotated. These proteins, called alternative failed to be annotated notably due the short length their open reading frame (less than 100 codons) or enforced rule establishing messenger RNAs (mRNAs) are monocystronic. Several biologically active molecules and seem involved in a wide range biological functions. However, genome exploration proteome is still limited few species. In present...