Abstract BACKGROUND: As a potential source of biomass, Jerusalem artichoke has been studied for bioethanol production; however, thus far it not investigated the production other liquid biofuels, such as biodiesel. This work aims to develop novel approach biodiesel from tuber using heterotrophic microalgae. RESULTS: In this study, Chlorella protothecoides utilized hydrolysate carbon and accumulated lipid in vivo , with content high 44% by dry mass, conversion ratio about 25% 4‐day scale...

Microalgae-derived biodiesel is a promising substitute for conventional fossil fuels. In particular, the green alga Chlorella protothecoides sp. 0710 regarded as one of best candidates commercial manufacture microalgae-derived biofuel. This due not only to its ability live autotrophically through photosynthesis, but also capacity produce large amount biomass and lipid fermentation glucose. However, until present study, neither genome sequence nor platform required molecular manipulations...

A model was constructed consisting of clinical and serum variables to discriminate between hepatitis B e antigen (HBeAg)-positive chronic (CHB) patients with without significant fibrosis (stages 2-4 vs. stages 0-1). Consecutive treatment-naive CHB who underwent liver biopsy were divided into 2 sequential groups: a training group (n = 200) validation 172). Multivariate analysis identified α2-macroglobulin, age, gamma glutamyl transpeptidase, hyaluronic acid as independent predictors fibrosis....

In recent years, a great interest has been dedicated to the development of noninvasive predictive models substitute liver biopsy for fibrosis assessment and follow-up. Our aim was provide simpler model consisting routine laboratory markers predicting in patients chronically infected with hepatitis B virus (HBV) order optimize their clinical management.Liver staged 386 chronic HBV carriers who underwent testing. Correlations between stage were statistically assessed. After logistic regression...

The alga Chlorella protothecoides is known to produce oil suitable for biodiesel preparation by heterotrophic cultivation in media containing glucose as a carbon source. In this study, sugar cane juice was used alternative supply production. As result, the highest content of 53.0% cell dry weight achieved. Fermentation 5 L bioreactor showed that algae using hydrolysate (SCH) grow faster than glucose. Conversion ratios sugar/biomass and sugar/oil SCH were 15.2 8.8% higher glucose,...

FAT4, a cadherin-related protein, was shown to function as tumour suppressor; however, its role in human gastric cancer remains largely unknown. Here, we investigated the of FAT4 and examined underlying molecular mechanisms. The expression evaluated by immunohistochemistry, western blotting, qRT–PCR relation clinicopathological characteristics patients. effects silencing on cell proliferation, migration, invasion were assessed MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay,...

In this study, we investigated the role of ubiquitin-specific protease 22 (USP22) in growth and progression gastric cancer (GC).USP22 mRNA protein levels were significantly higher GC tissue samples cell lines than adjacent noncancerous a normal mucosal epithelial line (GES1), respectively.USP22 knockdown decreased vitro survival, proliferation, migration, invasiveness cells compared with controls.Western blot analysis control USP22-silenced showed that USP22 modulates...

The diversified physiological responses in cyanobacteria under ultraviolet-B (UV-B) radiation have been broadly researched. changes the metabolic control mechanisms hidden behind these traits still need to be further investigated. This research attempts identify some of internal several stressful phenotypes such as a decreased growth rate, an impaired photosystem, and degradation photosynthetic pigments. Different expression levels proteins cytoplasm Synechocystis sp. PCC 6803 short-term...

Abstract BACKGROUND: The objectives of this study were to identify and validate the diagnostic value N‐glycan markers in colorectal cancer (CRC) uncover their underlying molecular mechanism. METHODS: In total, 347 individuals, including patients with CRC, adenoma, healthy controls, divided randomly into a training group (n = 287) retrospective validation groups 60). Serum profiling was analyzed by DNA sequencer‐assisted/flurophore‐assisted carbohydrate electrophoresis (DSA‐FACE). Two models...

// Jun-Tang Li 1, 2, 3, * , Lin-Tao Jia Ning-Ning Liu Xiao-Shan Zhu 1 Qin-Qin Xiu-Li Wang Feng Yu Yan-Li An-Gang Yang 2 Chun-Fang Gao Centre of Inflammation and Cancer Research, 150th Central Hospital PLA, Luoyang, Henan 471031, China State Key Laboratory Biology, Department Immunology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 3 Biochemistry Molecular These authors have contributed equally to this work Correspondence to: Gao, e-mail: gaocfsoul@163.com Yang,...

Abstract Introduction The onset of distal metastasis, which underlies the high mortality breast cancers, warrants substantial studies to depict its molecular basis. Nuclear factor activated T cells 5 (NFAT5) is upregulated in various malignancies and critically involved migration invasion neoplastic cells. Nevertheless, metastasis-related events potentiated by this transcriptional mechanism responsible for NFAT5 elevation carcinoma remain be fully elucidated. Methods correlation with cancer...

Highlights•Sirt5+ subset in wild-type Kras colorectal tumors determines multidrug resistance•Sirt5-mediated demalonylation and inactivation of SDHA lead to succinate accumulation•Succinate binds activates TrxR2 maintain chemotherapy resistance•Succinate inhibits aKG-dependent dioxygenases regulate cetuximab resistanceSummaryA major obstacle for successful management patients with carcinoma (CRC) is resistance anti-cancer cytotoxic treatments. Here, we identified a mechanism CRCs based on the...

// Ling Qin 1,* , Zhi-qiang Yao 2,* Qi Chang 3,* Ya-li Zhao Ning-ning Liu Xiao-shan Zhu 2 Qin-qin Li-feng Wang 4 An-gang Yang 5 Chun-fang Gao and Jun-tang Li 2,3,4,5 1 Department of Hematology, First Affiliated Hospital, College Clinical Medicine Henan University Science Technology, Luoyang, Henan, China Centre Inflammation Cancer Research, 150th Central Hospital PLA, 3 Biomaterial Biophysics Institute Training Medicine, State Key Laboratory Biology, Biochemistry Molecular Fourth Military...

Diagnosis of lung cancer at an early disease stage is important for successful treatment and improving the outcome patients. To improve its prognosis, we attempted to explore novel tools screening serum biomarkers distinguish from healthy individuals by protein profiles a classification tree algorithm.Serum samples were applied metal affinity chips generate mass spectra surface-enhanced laser desorption/ionization (SELDI) time-of-flight spectrometry. Protein peak identification clustering...

In evidence-based medicine (EBM), systematic reviews and meta-analyses have been widely applied in biological medical research. Moreover, the most popular application of this field may be to examine diagnostic (sensitivity specificity) prognostic (hazard ratio (HR) its variance, standard error (SE) or confidence interval (CI)) test accuracy. However, conducting such analyses requires not only a great deal time but also an advanced professional knowledge mathematics, statistics computer...

Colorectal cancer (CRC) remains a major worldwide cause of cancer-related morbidity and mortality largely due to the insidious onset disease. The current clinical procedures utilized for disease diagnosis are invasive, unpleasant, inconvenient. Hence, need simple blood tests that could be used early detection is crucial its ultimate control prevention.The present work case-control study focused on proteomic analysis serum healthy volunteers CRC patients by ClinProt profiling technology based...

It is still a subject of debate whether hepatocytes have the ability to express TGF-beta. Therefore, we investigated in freshly isolated and monolayer cultures rat expression TGF-beta isoform s at RNA protein level applying RT-PCR, immunocytochemistry, immunoblotting, functional assays TFG-beta 1, -beta 2, 3 transcripts were detected cultured cells, m increased up 48/72 h, but 1 absent cells. Using APAAP stainings proteins all three isoforms observed hepatocyte from 5-96 liver situ cell...

The early diagnosis of hepatocellular carcinoma (HCC) is great clinical desirable due to lack specific and sensitive markers. Alterations in the sugar chains glycoprotein synthesized by liver contribute molecular basis abnormalities carcinogenesis. This study aims construct assess diagnostic value N-glycan based model HCC identification follow-up. A total 393 subjects including HBV-related HCC, fibrosis healthy controls were recruited. Follow-up was carried out before after surgical...

We investigated the effect of 1.4% isoflurane (ISO) on development inflammation and apoptosis caused by zymosan (ZY) in mice. found that ZY-challenged mice exhibited significant body weight loss, markedly high mortality, lung injury characterized deterioration histopathology, histologic scores, wet-to-dry ratio after ISO treatment. dramatically attenuated ZY-induced neutrophil recruitment inflammation, as evidenced reduced levels total cells, neutrophils, proinflammatory cytokines (i.e.,...