A5039242992- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- MicroRNA in disease regulation
- Glioma Diagnosis and Treatment
- Cancer-related gene regulation
- CAR-T cell therapy research
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Neuroblastoma Research and Treatments
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
Horizon Discovery (United Kingdom)
2015-2024
Horizon Discovery Group (United States)
2014-2020
Keele University
1996-2017
Imperial College London
2017
Macmillan Cancer Support
2004-2011
St. James's Hospital
2009
Massey University
2008
Kleijnen Systematic Reviews (United Kingdom)
2006-2007
Addenbrooke's Hospital
2000-2006
University of Cambridge
2003-2006
There is increasing evidence for a central role in mammalian apoptosis of the interleukin-1β– converting enzyme (ICE) family cysteine proteases, homologues product nematode "death" gene, ced-3. Ced-3 thought to act as an executor rather than regulator programmed cell death nematode. However, it not known whether ICE-related proteases (IRPs) are involved execution or regulation apoptosis. Moreover, absolute requirement one more IRPs has yet be established. We have used two cell-permeable...
Abstract Image-based profiling has emerged as a powerful technology for various steps in basic biological and pharmaceutical discovery, but the community lacked large, public reference set of data from chemical genetic perturbations. Here we present generated by Joint Undertaking Morphological Profiling (JUMP)-Cell Painting Consortium, collaboration between 10 companies, six supporting two non-profit partners. When completed, dataset will contain images profiles Cell assay over 116,750...
Abstract Pooled CRISPR–Cas9 knock out screens provide a valuable addition to the methods available for novel drug target identification and validation. However, where gene editing is targeted amplified loci, resulting multiple DNA cleavage events can be cause of false positive hit identification. The generation nuclease deficient versions Cas9 has enabled development two additional techniques – CRISPR interference (CRISPRi) activation (CRISPRa) that enable repression or overexpression,...
Abstract Components of the type II CRISPR–Cas complex in bacteria have been used successfully eukaryotic cells to facilitate rapid and accurate cell line engineering, animal model generation functional genomic screens. Such developments are providing new opportunities for drug target identification validation, particularly with application pooled genetic screening. As is a relatively screening tool, it important assess its functionality number different lines analyse potential improvements...
Abstract Introduction Kirsten Rat Sarcoma Viral Oncogene Homolog ( KRAS ) mutations occur in approximately one-third of colorectal (CRC) tumours and have been associated with poor prognosis resistance to some therapeutics. In addition the well-documented pro-tumorigenic role mutant Ras alleles, there is evidence suggesting that not all are equal position type amino acid substitutions regulate biochemical activity transforming capacity mutations. Objectives To investigate metabolic signatures...
Cell cycle inhibitors are promising agents to prevent or treat human coronary in-stent stenosis (ISS). However, their lack of specificity for ISS vascular smooth muscle cells (VSMCs) may inhibit medial VSMC proliferation and suppress vessel healing.To identify inhibitor targets that differentially regulate vs. VSMCs, we examined cell regulation in VSMCs derived from (A) normal media, (B) sites (C) primary atherosclerotic plaques (P-VSMCs) using time-lapse videomicroscopy, flow cytometry,...
Drug-eluting stents containing the immunosuppressant rapamycin markedly inhibit in stent restenosis (ISR). However, molecular mechanisms that underlie its effect on ISR-derived vascular smooth muscle cells (VSMCs), as opposed to normal VSMCs, are unknown. Specifically, ISR-VSMCs have altered cell cycle regulation, may arrest these via novel pathways.We isolated human VSMCs from sites of ISR, and examined proliferation using MTT assay, time lapse videomicroscopy flow cytometry. Regulation...