A5088521802- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Pancreatic function and diabetes
- Chromatin Remodeling and Cancer
- Renal and related cancers
- Neuroscience and Neuropharmacology Research
- Barrier Structure and Function Studies
- Neonatal and fetal brain pathology
- Photoreceptor and optogenetics research
- Congenital heart defects research
- Animal Genetics and Reproduction
- Glioma Diagnosis and Treatment
- Immune responses and vaccinations
- Calcium signaling and nucleotide metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- interferon and immune responses
- Retinal Diseases and Treatments
- Genetic Mapping and Diversity in Plants and Animals
- Genomic variations and chromosomal abnormalities
- Single-cell and spatial transcriptomics
University of California, Santa Barbara
2014-2023
University of Massachusetts Chan Medical School
2019-2020
Neuropsychiatric Research Institute
2020
Bioscience Research
2014
Harvard University
2012
Stanford University
2012
Boston Children's Hospital
2012
Microglia, a resident CNS macrophage, are dynamic cells, constantly extending and retracting their processes as they contact functionally regulate neurons other glial cells. There is far less known about microglia–vascular interactions, particularly under healthy steady-state conditions. Here, we use the male female mouse cerebral cortex to show that higher percentage of microglia associate with vasculature during first week postnatal development compared older ages timing these associations...
Microglia play important roles in shaping the developing CNS, and at early stages they have been proposed to regulate progenitor proliferation, differentiation, neuronal survival. However, these studies reveal contradictory outcomes, highlighting complexity of cell–cell interactions. Here, we investigate microglia function during embryonic mouse retina development, where only microglia, progenitors, neurons are present. In both sexes, determine that primarily interact with retinal find...
The retina contains two populations of cholinergic amacrine cells, one positioned in the ganglion cell layer (GCL) and other inner nuclear (INL), that together comprise ∼1/2 a percent all retinal neurons. present study examined genetic control number distribution between these layers. total cells was quantified C57BL/6J A/J inbred mouse strains, 25 recombinant strains derived from them, variations their ratio (GCL/INL) across were mapped to genomic loci. found vary 27,000 40,000 despite...
Abstract Sox2 is a transcriptional regulator that highly expressed in retinal astrocytes, yet its function these cells has not previously been examined. To understand role, we conditionally deleted from the population of astrocytes and examined consequences on development. We found deletion does alter migration but it impairs their maturation, evidenced by delayed upregulation glial fibrillary acidic protein (GFAP) across retina. The centro‐peripheral gradient angiogenesis also Sox2‐CKO...
Genetic variants modulate the numbers of various retinal cell types in mice. For instance, there is minimal variation number rod bipolar cells (RBCs) two inbred strains mice (A/J and C57BL/6J), yet their F1 offspring contain significantly more RBCs than either parental strain. To investigate genetic source this variation, we mapped across 24 genetically distinct recombinant (RI) (the AXB/BXA strain-set), seeking to identify quantitative trait loci (QTL). We then sought candidate genes...
The present study interrogated a quantitative trait locus (QTL) on Chr 4 associated with the population sizes of two types bipolar cell in mouse retina. This was identified by quantifying number rod cells and Type 2 cone across panel recombinant inbred (RI) strains mice derived from laboratory strains, C57BL/6J (B6/J) A/J, mapping proportion that variation number, for each type, to this shared locus. There, we candidate gene X Kell blood group precursor related family member 8 homolog...
Sequence variants modulating gene function or expression affect various heritable traits, including the number of neurons within a population. The present study employed forward-genetic approach to identify candidate causal genes and their sequence controlling one type retinal neuron, AII amacrine cell. Data from twenty-six recombinant inbred (RI) strains mice derived parental C57BL/6J (B6/J) A/J laboratory were used genomic loci regulating cell number. Large variation in is across RI...
ABSTRACT Microglia, the resident macrophages of central nervous system (CNS), are dynamic cells, constantly extending and retracting their processes as they contact functionally regulate neurons other glial cells. There is far less known about microglia-vascular interactions, particularly under healthy steady-state conditions. Here, we use male female mouse cerebral cortex to show that a higher percentage microglia associate with vasculature during first week postnatal development compared...
Abstract DDX3X is an ATP-dependent RNA helicase that belongs to extended family of D-E-A-D motif (DEAD-box) containing proteins whose functions have been implicated in key biological processes ranging from transcription and splicing the regulation translation initiation. Through whole exome sequencing 94 primary medulloblasotmas, we identified as one most common recurrently mutated genes medulloblastoma. These mutations predominantly occur WNT pathway medulloblastomas which concurrently...