A5041994080- Diabetes Management and Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Advanced Glycation End Products research
- Diabetes and associated disorders
- Hemoglobinopathies and Related Disorders
- Trace Elements in Health
- Iron Metabolism and Disorders
- Diabetes Treatment and Management
- Folate and B Vitamins Research
- Microfluidic and Capillary Electrophoresis Applications
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Pancreatic function and diabetes
- Biochemical effects in animals
- Biosensors and Analytical Detection
- Neonatal Health and Biochemistry
- Microfluidic and Bio-sensing Technologies
- Helicobacter pylori-related gastroenterology studies
- Blood Pressure and Hypertension Studies
- Heavy Metal Exposure and Toxicity
- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Cytomegalovirus and herpesvirus research
- Metabolomics and Mass Spectrometry Studies
- Diabetic Foot Ulcer Assessment and Management
- Spectroscopy Techniques in Biomedical and Chemical Research
Louisiana State University
2000-2024
Louisiana State University Health Sciences Center New Orleans
2015-2024
Children's Hospital of New Orleans
2008-2020
Baton Rouge Clinic
2020
University of New Orleans
2018
Sanofi (Germany)
2011
Center for Children
2007
University Medical Center New Orleans
1998-2003
Texas Children's Hospital
2003
Baylor College of Medicine
2003
OBJECTIVE—We hypothesized that biological variation in HbA1c, distinct from attributable to mean blood glucose (MBG), would predict risk for microvascular complications the Diabetes Control and Complications Trial (DCCT). RESEARCH DESIGN AND METHODS—A longitudinal multiple regression model was developed MBG HbA1c measured 1,441 DCCT participants at quarterly visits. A hemoglobin glycation index (HGI = observed - predicted HbA1c) calculated each visit assess based on directional deviation of...
OBJECTIVE This study tested the hypothesis that intensive treatment in Action to Control Cardiovascular Risk Diabetes (ACCORD) trial disproportionately produced adverse outcomes patients with diabetes a high hemoglobin glycation index (HGI = observed HbA1c − predicted HbA1c). RESEARCH DESIGN AND METHODS ACCORD was randomized controlled of 10,251 type 2 assigned standard or goals 7.0% 7.9% (53 63 mmol/mol) and less than 6% (42 mmol/mol), respectively. In this ancillary study, linear...
Mean blood glucose (MBG) over 2-3 months is a strong predictor of HbA(1c) (A1C) levels. Glucose instability, the variability levels comprising MBG, and biological variation in A1C (BV) have also been suggested as predictors independent MBG. To assess relative importance BV, instability on A1C, we analyzed patient data from Diabetes Control Complications Trial (DCCT).A profile set sample for were collected quarterly course DCCT each participant (n = 1,441). The consisted seven samples, one...
The mechanism of zinc absorption has not been delineated, but kinetic studies show that both passive and carrier-mediated processes are involved. We have identified a low molecular mass zinc-binding protein in the soluble fraction rat intestinal mucosa could function as an intracellular carrier. was detected liver or pancreas, suggesting role specific to intestine. binds during transmucosal transport shows signs saturation at higher luminal concentrations, characteristics consistent with...
A rapid data-collection system for determining egg weight and specific gravity is described. The method links a microcomputer an electronic balance to facilitate determination by the displaced water (CADW) or Archimedes' method. Measurements obtained this technique were compared with those determined saline flotation Repeatability measures showed both methods be relatively precise. CADW was faster reduced opportunities clerical error.
OBJECTIVE Mean blood glucose (MBG) and MBG-independent factors both influence A1C levels. Race was related to independent of MBG in adults. The goal this study determine if racial disparity exists children with diabetes. RESEARCH DESIGN AND METHODS Participants included 276 type 1 were obtained from multiple clinic visits, a hemoglobin glycation index (HGI) (an assessment levels MBG) calculated. HGI analyzed controlling for age, diabetes duration, MBG. RESULTS African Americans had...
Inflammation is associated with higher glycated hemoglobin (HbA1c) levels. Whether the relationship independent of blood glucose concentration remains unclear. The glycation index (HGI) was used to test hypothesis that interindividual variation in HbA1c inflammation. This study nondiabetic adults from National Health and Nutrition Examination Survey (1999–2008). A subsample participants estimate linear regression between fasting plasma (FPG). Predicted were calculated for 7323 by inserting...
Soros AA, Chalew SA, McCarter RJ, Shepard R, Hempe JM. Hemoglobin glycation index: a robust measure of hemoglobin A1c bias in pediatric type 1 diabetes patients. Background: The index (HGI) assesses biological variation after accounting for the effect mean blood glucose (MBG). Previous studies minimized analytical that could mask and showed HGI was consistent within individuals over time positively associated with risk microvascular complications. We tested hypothesis can be assessed by...
To evaluate the relationship between skin advanced glycation end products (sAGEs) with mean blood glucose (MBG), hemoglobin A(1c) (HbA(1c)), and MBG-independent, between-patient differences in HbA(1c) among children type 1 diabetes.Children aged 5 to 20 years diabetes of at least year duration participated. At a clinic visit, sAGE was estimated noninvasively by measurement intrinsic fluorescence (SIF). SIF data were adjusted correct for variation pigmentation. examined statistically...
Abstract Capillary isoelectric focusing (cIEF) was used to identify and quantify major minor hemoglobin (Hb) variants. Whole blood (approximately 10 microL required) hemolysate analyzed with a commercial instrument equipped 50 microns (i.d.) x 27 cm coated capillary filled 20 g/L ampholytes (pH 6-8) in 4 methylcellulose (MC). Cathode anode solutions were mol/L NaOH 100 H3PO4 MC, respectively. Samples 40 nL) applied via autosampler by low-pressure injection, focused for 3 min at 30 kV,...
The A1C-Derived Average Glucose study recommended reporting A1C in estimated average glucose (eAG) equivalents. We compared eAG with self-monitored mean blood (MBG) to determine whether is systematically biased due biological variation the relationship between MBG and A1C.
Background Racial variation in the relationship between blood glucose and hemoglobin A1c (HbA1c) complicates diabetes diagnosis management racially mixed populations. Understanding why HbA1c is persistently higher blacks than whites could help reduce racial disparity outcomes. Objective Test hypothesis that neighborhood disadvantage associated with inflammation poor metabolic control a population of pediatric type 1 patients. Methods Patients (n = 86, 53 white, 33 black) were recruited from...
Fasting plasma glucose (FPG), 2-hour (2hPG) from a 75-g oral tolerance test (OGTT) and glycated hemoglobin (HbA1c) can lead to different results when diagnosing prediabetes diabetes. The Hemoglobin Glycation Index (HGI) quantifies the interindividual variation in glycation resulting discrepancies between FPG HbA1c. We used data Vitamin D Type 2 Diabetes (D2d) study calculate HGI, identify HGI-associated variables, determine how HGI affects diabetes diagnosis.A linear regression equation...
A high haemoglobin glycation index (HGI) is associated with greater risk for hypoglycaemia and chronic vascular disease. Standardizing how the HGI calculated would normalize results between research studies hospital laboratories facilitate clinical use of assessing risk.The difference an observed HbA1c a predicted obtained by inserting fasting plasma glucose (FPG) into regression equation describing linear relationship FPG in reference population. We used data from 2005-2016 U.S. National...
This study was prompted to investigate the intestinal localization and colonization of orally administered Escherichia coli Nissle 1917 (EcN) in piglets.EcN fed ten EcN-negative piglets (3 months) over seven consecutive days. Faecal samples were collected repeatedly tested for EcN-DNA by a combined culture/PCR assay viable EcN culture methods, respectively. detectable faeces all within first 24 h after it added feed. After administration had been stopped, presence faecal indicated that...
Abstract We have used capillary isoelectric focusing (cIEF) to diagnose and monitor hemoglobinopathies in children for over three years. This report describes a major revision of our original method ( Clin. Chem. 1994, 40 , 2288–2295) that improves the analysis hemoglobin (Hb) variants by cIEF, especially performance quantitation precision minor variants. The revised uses mixed ampholytes (2% pH 6‐8:3‐10; 10:1), lower viscosity methylcellulose (0.375%) solution, between‐sample conditioning...
To assess the role of distal nephron apical Na channel (ENaC) gene expression in wasting by immature kidney, ENaC α-, β-, and γ-subunit mRNA levels were examined rat RT-PCR. In microdissected segments, all three subunit mRNAs detected convoluted tubule, connecting cortical collecting duct, outer medullary duct. The inner duct other segments consistently negative. quantified kidneys at different developmental stages multiplex RT-PCR with “primer dropping,” endoplasmic reticulum-specific...
Male obese Zucker Diabetic Fatty (ZDF) rats develop type 2 diabetes around eight weeks of age, and are widely used as a model for human its complications. The objective the study was to test whether complications manifested in kidney nerves ZDF really correspond diabetic their being related hyperglycaemic state. Four groups were used. One lean (Fa/?) one (fa/fa) untreated group served non-diabetic controls. In two further rats, prevented by pioglitazone or delayed food restriction. All...