A5069703142- Iron Metabolism and Disorders
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Pancreatic and Hepatic Oncology Research
- Alcohol Consumption and Health Effects
- Immune Cell Function and Interaction
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal disorders and treatments
- Acute Myeloid Leukemia Research
- Liver Disease and Transplantation
- Peroxisome Proliferator-Activated Receptors
- Mesenchymal stem cell research
- Hepatitis B Virus Studies
- Pancreatitis Pathology and Treatment
- Immunotherapy and Immune Responses
- Liver physiology and pathology
- Glycosylation and Glycoproteins Research
- Hematopoietic Stem Cell Transplantation
- Proteoglycans and glycosaminoglycans research
- Gastric Cancer Management and Outcomes
- Folate and B Vitamins Research
- Toxin Mechanisms and Immunotoxins
- Chronic Lymphocytic Leukemia Research
International University of Health and Welfare
2016-2024
Asahikawa Medical University
2001-2016
Asahikawa Medical College Hospital
2004-2013
Asahikawa University
2000-2012
Palmetto Hematology Oncology
2011-2012
Osaka Medical and Pharmaceutical University
2010
Toshiba (Japan)
2010
Massachusetts General Hospital
2010
Harvard University
2010
Third Way
2010
A reliable and inexpensive noninvasive marker of hepatic fibrosis is required in patients with nonalcoholic fatty liver disease (NAFLD). FIB4 index (based on age, aspartate aminotransferase [AST] alanine [ALT] levels, platelet counts) expected to be useful for evaluating fibrosis. We validated the performance a Japanese cohort NAFLD.The areas under receiver operating characteristic curves (AUROC) six other markers were compared, based data from 576 biopsy-proven NAFLD patients. Advanced was...
To identify predictive molecular markers for gemcitabine resistance, we investigated changes in the expression of four genes associated with transport and metabolism during development acquired resistance pancreatic cancer cell lines. The levels human equilibrative nucleoside transporter-1 (hENT1), deoxycytidine kinase (dCK), RRM1, RRM2 mRNA were analysed by real-time light cycler-PCR various subclones to gemcitabine. Real-time demonstrated that either RRM1 or progressively increased...
Previous reports have suggested that some probiotics inhibit tumorigenesis and cancer progression. However, the molecules involved not yet been identified. Here, we show culture supernatant of Lactobacillus casei ATCC334 has a strong tumour-suppressive effect on colon cells. Using mass spectrometry, identify ferrichrome as molecule produced by L. ATCC334. The is greater than cisplatin 5-fluorouracil, less an non-cancerous intestinal cells either those agents. A transcriptome analysis reveals...
We investigated the expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and role PPARgamma in cell growth human gastric cancer cells. Reverse transcription-polymerase chain reaction, Northern blot Western analyses showed that a line, MKN45, expressed mRNA protein. Luciferase assay MKN45 cells troglitazone, selective ligand for PPARgamma, transactivated transcription proliferator response element-driven promoter. Troglitazone or pioglitazone, ligands inhibited...
Several clinical studies have suggested that excess hepatic iron accumulation is a progressive factor in some liver diseases including chronic viral hepatitis and hemochromatosis. However, it not known whether iron-induced hepatotoxicity may be directly involved hepatitis, cirrhosis, cancer. The Long-Evans Cinnamon (LEC) rat, which accumulates copper the as patients with Wilson's disease, of mutant strain displaying spontaneous hemolysis, We found previously LEC rats harbored an additional...
Syndecans comprise a gene family of transmembrane proteoglycans that regulate cellular behavior through interactions with various effectors, including heparin-binding growth factors and insoluble matrix components. Syndecan-1, the most extensively studied, localizes in epithelial cells has been shown to present normal hepatocytes. However, little is known about change syndecan-1 expression human hepatocellular carcinoma (HCC). We investigated syndecan-1-protein by immunohistochemistry 57 HCC...
In chronic inflammation it is reported that serum iron depleted and hepatic increased because of reticuloendothelial system blockade. However, recent studies indicate parenchymal cells increase the uptake transferrin-bound after in vivo stimulation with bacterial lipopolysaccharide, suggesting endotoxemia itself or lipopolysaccharide-induced production inflammation-related cytokines may also be responsible for this phenomenon. study actions on synthesis iron-binding proteins (transferrin...
Abstract Serum and tissue miR-21 expression in patients with breast cancer (BC) is a useful biomarker for diagnosis, progression, treatment. Matrix metalloproteinase-1 (MMP-1) also important carcinogenesis. However, MMP-1/CD63 urine exosomes these have not been examined. Urine samples were collected from BC 26 healthy females. Urinary isolated confirmed by western blotting anti-CD63 antibody electron microscopy observation. MiR-21 was examined quantitative RT-PCR blotting, respectively....
Multiple lesions of intraductal papillary-mucinous tumor the pancreas (IPMT) in same often are encountered. To elucidate field (multicentric) cancerization and clonality IPMT, clonal analyses IPMT its precursor lesion ductal hyperplasia were performed. K-ras codon 12 mutations X-chromosome inactivation human androgen receptor gene (HUMARA) investigated.Paraffin embedded tissue samples from pancreata 37 patients who underwent resection for IPMTs microdissected manually or by laser capture...
Syndecans comprise a gene family of transmembrane proteoglycans that regulate cellular behavior through interactions with various effectors, including heparin-binding growth factors and insoluble matrix components. Syndecan-1, the most extensively studied, localizes in epithelial cells has been shown to present normal hepatocytes. However, little is known about change syndecan-1 expression human hepatocellular carcinoma (HCC). We investigated syndecan-1-protein by immunohistochemistry 57 HCC...
Abstract The prognosis for patients with bile duct cancer (BDC) remains poor. Although BDC cells are essentially radioresistant, recent reports have suggested that radiation therapy, in addition to its palliative role the management of BDC, may improve patient survival. A better understanding mechanisms lead cellular radioresistance assist development more effective therapies based on radiotherapy combination radiosensitizing agents. serine/threonine kinase AKT/protein B, a downstream...
Background: It is common for alcoholic patients to have excess iron accumulation in the liver, which may contribute development of liver disease (ALD). However, mechanism hepatic uptake ALD still obscure. Recently, a novel iron‐regulatory hormone hepcidin was found that suppresses absorption from small intestine and release macrophages. To elucidate contribution toward ALD, we examined whether alcohol loading affects expression both an ethanol‐fed mouse model. Methods: Serum prohepcidin...
A glutathione-S-transferase-pi (GST-pi) immunoradiometric assay was devised as a potential marker for serodiagnosis of malignant disease. Elevated serum GST-pi levels were observed in patients with various gastrointestinal malignancies including gastric, esophageal, colonic, pancreatic, hepatocellular, and biliary tract cancers. Patients benign diseases had normal GST-pi, but some chronic hepatitis cirrhosis slightly elevated levels. Over 80% Stage III or IV gastric cancer even about 50%...