We provide a novel and versatile signaling transduction strategy in the fluoroimmunoassay through regulating interaction between graphene (Gr) quantum dots (GQDs), demonstrate its feasibility sensitive detection of human immunoglobulin G (IgG).

Large-scale single-crystalline ultrathin boron nanosheets (UBNSs, ≈10 nm) are fabricated through an effective vapor-solid process via thermal decomposition of diborane. The UBNSs have obvious advantages over thicker nanomaterials in many aspects. Specifically, the demonstrate excellent field emission performances with a low turn-on field, Eto, 3.60 V μm-1 and good stability. Further, dependence (turn-on field) Eto/(threshold Ethr work function, Φe, on temperature is investigated possible...

ConspectusPathogens have long presented a significant threat to human lives, and hence the rapid detection of infectious pathogens is vital for improving health. Current methods lack means detect in simple, rapid, reliable manner at time point need. Functional nucleic acids (FNAs) potential overcome these limitations by acting as key components point-of-care (POC) biosensors due their distinctive advantages that include high binding affinities specificities, excellent chemical stability,...

Abstract We report on the first effort to select DNA aptamers from a circular library, which resulted in discovery of two high‐affinity that recognize glutamate dehydrogenase (GDH) Clostridium difficile, an established antigen for diagnosing difficile infection (CDI). One aptamer binds effectively both and linear forms, other is functional only configuration. Interestingly, these different epitopes GDH, demonstrating advantage selecting libraries. A sensitive diagnostic test was developed...

clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems are increasingly used in biosensor development. However, directly translating recognition events for non-nucleic acid targets by CRISPR into effective measurable signals represents an important ongoing challenge. Herein, it is hypothesized and confirmed that RNAs (crRNAs) a circular topology efficiently render Cas12a incapable of both site-specific double-stranded DNA cutting nonspecific single-stranded trans...

Functional nucleic acids (FNAs), such as DNAzymes and DNA aptamers, can be engineered into circular forms for improved performance. Circular FNAs are promising candidates bioanalytical biomedical applications due to their intriguing properties of enhanced biological stability compatibility with rolling circle amplification. They typically made from linear single-stranded (ss) molecules via ligase-mediated ligation. However, it remains a great challenge synthesize ssDNA in high yield inherent...

Abstract Protein biomarkers often exist as degradation fragments in biological samples, and affinity agents derived using a purified protein may not recognize them, limiting their value for clinical diagnosis. Herein, we present method to overcome this issue, by selecting aptamers against degraded form of the toxin B protein, which is marker diagnosing toxigenic Clostridium difficile infections. This approach has led isolation DNA aptamer that recognizes B, fresh spiked into human stool...

Adenosine 5'-triphosphate (ATP) is a central extracellular signaling agent involved in various physiological and pathological processes. However, precise measurements of the temporal spatial components ATP dynamics are lacking due primarily to limitations available methods for detection. Here, we report on first effort design self-phosphorylating DNAzyme (SPDz) sensor fluorescence imaging ATP. In response ATP, SPDz sensors exhibit subsecond kinetics, extremely high specificity, micromolar...

High‐quality NdB 6 nanostructures with a low work function are successfully synthesized via an one‐step catalyst‐free chemical vapor deposition process. Field emission properties of these (curve nanowires, short‐straight nanorods, long‐straight and nanoneedles) systematically investigated found to be strongly affected by the tip morphologies temperatures. The nanoneedles sharp tips demonstrate lowest turn‐on (2.71 V/μm) threshold (3.60 electric fields, as well high current density (5.37...

The composition of protein corona affects the behavior and fate nanoparticles in biological systems, which strongly relates to intrinsic properties proteins. Here, three types MXene Ti3C2Tx nanosheets are prepared by different etching methods, certain physicochemical characteristics before after exposure human plasma (HP) characterized. with coronas suffer more easily from aggregation than pristine Ti3C2Tx. LC-MS/MS-based label-free proteomic analysis reveals a high overlap functions but...

Rapid detection of pathogenic bacteria is extremely important for public health and safety. Here, we describe the first time an integrated origami paper-based analytical device (PAD) incorporating cell lysis, molecular recognition, amplification visual Escherichia coli (E. coli). The features three components: paper its ability to extract protein molecules nonspecifically from cells, DNA superstructures their immobilize RNA-cleaving DNAzymes (RCDs) but undergo target-induced RNA cleavage on...

Phosphorus is a key element responsible for eutrophication, and its measurement speciation critical topic in analytical chemistry. Most research efforts have been devoted to detecting phosphate (P(V)), while few reports on phosphite (P(III)) are available, making it difficult sensor-based understanding of the phosphorus cycle. This study presents fluorescent "turn-on" sensor quantitative highly selective analysis based different coordination strength N P lone-pair electrons toward nickel...

CRISPR-Cas technologies have emerged as powerful biosensing tools for the sensitive and specific detection of non-nucleic acid targets. However, existing strategies suffer from poor compatibility across diverse targets due to complicated engineering crRNA DNA activator required activity regulation. Herein, we report a novel straightforward strategy designing CRISPR-Cas12a-based biosensors that function by switching structures single-stranded (ss)DNA/CRISPR-Cas12a assembly...

The development of simple and accurate detection uracil-DNA glycosylase (UDG) is great significance for early clinical diagnosis biomedical research. Here, we on the first effort introduced uracil bases into rolling circle amplification (RCA) reaction to produce functionalized pure DNA hydrogel (PDH) UDG detection. During RCA process, methylene blue (MB) molecules as indicators were encapsulated PDH. addition can remove PDH generate abasic sites, which are further cleaved with assistance...

DNAzyme Operation was uniquely exploited to Enhance the Specificity of CRISPR/Cas13a system (DOES - CRISPR). The versatility DOES-CRISPR demonstrated for detection microRNAs, CYP2C19*17 gene, SARS-Cov-2 variants, and Omicron subtypes.