A5037089876- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical Analysis and Sensing Techniques
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Veterinary Medicine and Surgery
- Diet and metabolism studies
- Virus-based gene therapy research
- Veterinary Oncology Research
- Diabetes Management and Research
- Dermatology and Skin Diseases
- Human-Animal Interaction Studies
- Pancreatic function and diabetes
- Advancements in Transdermal Drug Delivery
- Neuropeptides and Animal Physiology
- Diabetes Treatment and Management
- Muscle metabolism and nutrition
- Infrared Thermography in Medicine
- Sarcoma Diagnosis and Treatment
- Myofascial pain diagnosis and treatment
- Cancer, Hypoxia, and Metabolism
- Receptor Mechanisms and Signaling
- Pancreatitis Pathology and Treatment
- Thermal Regulation in Medicine
- Peroxisome Proliferator-Activated Receptors
Nippon Veterinary and Life Science University
2012-2024
Advanced Neural Dynamics (United States)
2024
University of Veterinary Medicine
2015
Hokkaido University
2000-2007
Laboratoire de Biochimie
2005
Advance (Japan)
2002
Morinaga Institute of Biological Science
2002
Obesity is the most common nutritional disorder in small animals. To establish a computed tomographic (CT) method for assessment of visceral and subcutaneous fat content dog, CT analysis was performed normal obese beagles. Fat area measured by level detection at varied attenuation ranges compared with body estimated deuterium oxide dilution method. L3 using range −135/−105 Hounsfield unit had best correlation ( r =0.98). Regional distribution almost same between dogs, more accumulation L1–S1...
Abstract Objective —To evaluate the relationship between plasma leptin concentration and body fat content in dogs. Animals —20 spayed female Beagles that were 10 months old at start of experiment. Procedure —Dogs kept under regulated feeding exercise conditions for 21 weeks, resulting a wide range weights, condition scores (BCS), subcutaneous thicknesses. Plasma was measured by use canine leptin- specific ELISA test to its correlation estimated deuterium oxide dilution method. concentrations...
Leptin is a protein synthesized and secreted primarily by adipocytes, the circulating leptin concentration elevated in obese humans rodents. Recently, we have established sandwich enzyme-linked immunosorbent assay for canine leptin. In present study, plasma concentrations were measured experimentally developed beagles clinically dogs. When 5 male given high-energy diet 3 months, all of them became significantly increased from 2.4 ± 1.2 to 4.9 0.9 ng/ml, positively correlating with body fat...
Adiponectin is an adipokine that specifically expressed in adipose tissues, directly sensitizes the body to insulin via specific receptors and its decreased plasma concentration responsible for resistance obese humans. Diabetes important problem also veterinary medicine, feline diabetes very similar human type 2 diabetes, which obesity risk factor. In present study, We obtained cDNA clones corresponding adiponectin receptor 1 (AD-R1), whose nucleotide deduced amino acid sequences were highly...
Hemangiosarcoma (HSA) is a malignant neoplasm that occurs in humans and canines with poor prognosis owing to metastatic spread, despite effective treatment. The frequency of spontaneous HSA development higher than humans. Therefore, canine useful model intractable human disease, which requires early detection an therapeutic strategy. A high the p110α phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit alpha (PIK3CA) mutations detected comprehensive genome‑wide analysis cases...
Leptin is a protein synthesized and secreted primarily by adipose tissue. The blood leptin concentration known to reflect body fat content in rodents, humans dogs, thereby useful for quantitative assessment of obesity. In the present study, we produced recombinant feline Escherichia coli transfected with cDNA cloned previously. molecular weight 16 kDa induced phosphorylation signal transducers activators transcription 3 (STAT3) cells expressing rat receptor. anti-feline antibody raised...
G protein-coupled receptor (GPR) 120 is an unsaturated fatty acid receptor, which associated with various physiological functions. It reported that the genetic variant of GPR120, p.Arg270His, detected more in obese people, and this variation functionally relates to obesity humans. Obesity a common nutritional disorder also dogs, but factors have not ever been identified dogs. In study, we investigated molecular structure canine GPR120 searched for candidate variants may relate Canine was...
To determine the role of leptin in endotoxin-induced anorexia ruminants, circulating levels were measured during acute experimental endotoxemia sheep. Injection bacterial lipopolysaccharide (450 ng/kg, i.v.) induced accompanied with fever and increases serum cortisol, insulin glucose which are known to stimulate secretion rodent human, while it did not affect at all. These results indicate that sheep differentially regulated from those might be involved
Pancreatitis is a common exocrine pancreatic disease in dogs, and the lipase immunoreactivity (PLI) test used for diagnosis. Enzyme catalytic assay thought to have low specificity, but activity with increased specificity has been developed human clinical chemistry. We measured serum of 65 client-owned dogs using newly FUJI DRI-CHEM slide compared results their PLI concentrations. The showed good correlation (r=0.91), normal pancreatitis identified based on values were correctly separated...
Nesfatin-1 is an anorexic peptide derived from a precursor, nucleobindin-2 (NUCB2), which distributed in various organs, coexists with ghrelin the gastric X/A-like cells and closely relates to appetite control rodents humans. may be significant factor addressing satiety also veterinary medicine, however, there are few reports about nesfatin-1 dogs. In present study, we detected canine NUCB2/nesfatin-1 mRNA tissues, especially abundant pancreas, gastrointestinal tracts, testis cerebellum. We...
The proliferation of peripheral blood mononuclear cells (PBMC) containing both monocyte/macrophages and T lymphocytes increased after treatment with T-cell mitogen (concanavalin A: Con A). PBMC treated either leptin alone or combination ConA showed enhanced proliferative activity by 10-40%, compared those alone. In contrast, isolated lowered than the ConA-treated alone, indicating that induced production some cytokines from monocyte/macrophages, subsequently resulted in enhancement PBMC....
Monocyte chemoattractant protein-1 (MCP-1) is a member of the C-C family chemokines, which mobilizes monocytes from bone marrow to site inflammation. To evaluate clinical utility canine MCP-1 as blood test item, we measured serum concentrations in normal and ill dogs. Reference interval was established 115.6-176.9 pg/ml. Serum increased dogs affected with neoplastic (518.0 ± 84.8 pg/ml), inflammatory (257.0 42.5 pg/ml) or other diseases (360.3 45.2 pg/ml). The results showed high sensitivity...
Although androgen therapy resistance and poor clinical outcomes are seen in most canine prostate cancer cases, there only a few tools for analysing by using cell biological approach. Therefore, to evaluate androgen-independent neoplastic growth, new line (CHP-1) was established this study. CHP-1 over-expressed the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is human cancer. The xenograft also showed SGTA over-expression. shows receptor (AR)...
Serum leptin concentrations were measured in antenatal and postnatal cows housed at two different locations. The mean serum concentration was 9.2 +/- 0.6 ng/m l (n=22) one group, slightly lower the other (7.4 0.4 ng/ml, n=54), probably because of nutritional conditions between groups. There no consistent variation relation to menstrual cycle periparturient period both Moreover, during independent number delivery incidence mastitis milk fever. These results are quite from those rodents human,...