A5056556506- BRCA gene mutations in cancer
- Veterinary Oncology Research
- Immune Response and Inflammation
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Animal health and immunology
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- NF-κB Signaling Pathways
- Hemoglobin structure and function
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Adipose Tissue and Metabolism
- interferon and immune responses
- Prostate Cancer Treatment and Research
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Cancer Genomics and Diagnostics
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Blood properties and coagulation
- Dermatology and Skin Diseases
Hokkaido University
2015-2024
Iwate University
2000-2008
Okayama University
2008
The University of Tokyo
2008
Tottori University
2008
Chiba University
2003
Yamaguchi University
2003
Dokkyo University
2003
Gifu University
2002
Lexicon Pharmaceuticals (United States)
1997-1998
We have identified and characterized a novel member of the ankyrin-repeat family named 'molecule possessing ankyrin-repeats induced by lipopolysaccharide' (MAIL). The C-terminal portion MAIL shared high sequence homology with I kappa B family. Intraperitoneal injection lipopolysaccharide (LPS) into mice rapidly (<0.5 h) mRNA in various tissues, particularly spleen, lymph node, lung. Ectopically expressed was localized nucleus, remarkably potentiated LPS-induced expression secretion...
Abstract CCL2, also referred to as MCP-1, is critically involved in directing the migration of blood monocytes sites inflammation. Consequently, excessive CCL2 secretion has been linked many inflammatory diseases, whereas a lack expression severely impairs immune responsiveness. We demonstrate that IκBζ, an atypical IκB family member and transcriptional coactivator required for selective subset NF-κB target genes, key activator Ccl2 gene. IκBζ-deficient macrophages exhibited impaired when...
Equine hepacivirus (EHcV) has been identified as a closely related homologue of hepatitis C virus (HCV) in the United States, Kingdom, and Germany, but not Asian countries. In this study, we genetically serologically screened 31 serum samples obtained from Japanese-born domestic horses for EHcV infection subsequently 11 PCR-positive 7 seropositive samples. We determined full sequence genome, including 3' untranslated region (UTR), which had previously completely revealed. The polyprotein...
Cold exposure produces adaptive hyperplasia and growth of brown adipose tissue (BAT), the major site non-shivering thermogenesis in rodents, associated with increased angiogenesis this tissue. Vascular endothelial factor (VEGF), one most potent angiogenic factors, was found to be expressed abundantly BAT rat. When rats were exposed cold at 4 °C, VEGF mRNA level by 2-3-fold 1-4 h, but returned basal within 24 h. expression other tissues such as heart, kidney lung did not change after...
A macromolecular hemoglobin-binding protein, which was not detectable in normal bovine sera but appeared during acute phase inflammation, purified, characterized, and designated as haptoglobin (Hp).The purified protein had a molecular mass of 1,000-2,000 kDa, composed two kinds peptides, 20-kDa peptide (a chain) 36-kDa glycopeptide (B linked by disulfide bonds.Amino acid composition N-terminal sequence analyses revealed that both peptides were homologous to each counterpart human Hp.Studies...
Recently, the second mammalian chitinase, designated acidic chitinase (AMCase), has been identified in human, mouse, and cow. In contrast to earlier macrophage-derived (chitotriosidase), this is richly expressed gastrointestinal (GI) tract, suggesting its role digestion of chitin-containing foods as well defense against chitin-coated microorganisms parasites. This situ hybridization study first revealed cellular localization gut-type mouse chicken. adult mice, parotid gland, von Ebner's...
In spermatogenesis, Sertoli cells serve as supporting for the proliferation and differentiation of germ cells. However, it appears that cell function is regulated by adjacent spermatogonial in testis because expression lipocalin-2 mRNA, which encodes an iron-siderophore-binding protein, barely detectable cell-deficient W/Wv mice, more abundantly expressed jsd/jsd mice. By employing a coculture system comprising immortalized (designated Sertoli-B) c-Kit(+) from 7-d-old mouse testis, we found...
The nucleoprotein (NP) possesses regions that are highly conserved among influenza A viruses, and has therefore been one of the target viral proteins for development a universal vaccine. It expected human or humanized antibodies will be made available prophylaxis, pre-emptive acute treatment infection. However, it is still unclear whether anti-NP antibody can confer protection against virus In this study, we generated transgenic mice expressing mAbs derived from lymphocytes patient infected...
<b><i>Objective:</i></b> To determine whether the anti-GM1 antibody IgG subclass (IgG1 to 4) is associated with clinical profiles and patterns of recovery in Guillain–Barré syndrome (GBS). <b><i>Methods:</i></b> The subclassification was examined compared data on 42 GBS patients positive for antibody. <b><i>Results:</i></b> Frequent subclasses were IgG1 (76%) IgG3 (31%). preceding gastroenteritis <i>Campylobacter jejuni</i> serology, whereas respiratory infection. Although severity at nadir...
Short-chain fatty acids in the intestinal lumen affect colonic cell proliferation as well function an energy source for epithelial cells. A novel transporter of monocarboxylates, Slc5a8, is expressed abundantly colon, where it may participate Na+-coupled absorption short-chain produced by bacterial fermentation dietary fiber. The present study examined cellular localization Slc5a8 murine gastrointestinal tract and kidney situ hybridization immunohistochemistry. signals were recognized...
Mammary tumors are the most common tumor type in intact female dogs. Recently, breast cancer 2 early onset (BRCA2) gene was proposed to be associated with tumorigenesis The expression level of BRCA2 is important for its DNA repair function mammalian cells, and linked mammary tissue. However, canine unclear. mRNA levels were compared between seven gland samples seventeen isolated from lower than samples. We attempted identify why decreased by promoter sequencing analysis; however, we did not...
Wild-type or mutated human β3-adrenergic receptor (Trp64Arg) cDNAs were stably expressed in mouse 3T3-L1 cells. Saturation binding study using a β-adrenergic ligand revealed that there was no significant difference the density and equilibrium dissociation constant between two cell lines. However, ability of mutant to accumulate cyclic AMP (cAMP) response isoproterenol much reduced Kact for cAMP accumulation lowered as compared wild type receptor. The amount alpha subunit stimulatory...
The hepatic synthesis of acute phase proteins in ruminants has been suggested to be regulated by some mechanisms different from those other species such as rodents and human. To explore possible fegulatory factors unique ruminants, we examined effects interleukin (IL)-6, IL-1 tumor necrosis factor (TNF), on haptoglobin (Hp) using a primary culture system bovine hepatocytes. After cultured hepatocytes were incubated the presence various concentrations cytokines, mRNA level albumin measured...
ABSTRACT Multimerization of the Hantaan virus nucleocapsid protein (NP) in virus-infected Vero E6 cells was observed a competitive enzyme-linked immunosorbent assay (ELISA). Recombinant and truncated NPs Hantaan, Seoul, Dobrava viruses lacking N-terminal 49 amino acids were also detected as multimers. Although 154 existed monomer, those Seoul formed The multimerized NP antigens could detect serotype-specific antibodies, whereas monomeric antigen not, suggesting that hantavirus epitope on...