A5081889445- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Biochemical Analysis and Sensing Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Nitric Oxide and Endothelin Effects
- Stress Responses and Cortisol
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Eicosanoids and Hypertension Pharmacology
- Immune Cell Function and Interaction
- Protein Kinase Regulation and GTPase Signaling
- Liver physiology and pathology
- Diabetes and associated disorders
- Receptor Mechanisms and Signaling
- Burkholderia infections and melioidosis
- Renal and related cancers
- Aldose Reductase and Taurine
- Brucella: diagnosis, epidemiology, treatment
- Tryptophan and brain disorders
- Angiogenesis and VEGF in Cancer
- Heat shock proteins research
- Phagocytosis and Immune Regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Cellular transport and secretion
Canadian Food Inspection Agency
2023
University of Manitoba
2011-2015
McMaster University
2014
Hokkaido University
2000-2008
Recent studies have suggested that proinsulin C-peptide improves vascular functions, possibly through nitric oxide (NO) production. To clarify the molecular mechanisms of NO production induced by C-peptide, we examined effects on and synthase expression in rat aortic endothelial cells connection with mitogen-activated protein kinase (MAPK) activation. Aortic were isolated from female Wistar rats, cultured to confluence, serum-starved for 24 h before treatment C-peptide. Nitric was measured...
It has been demonstrated that proinsulin C-peptide possesses several biological activities and its specific binding sites are present on the surface of cell membranes. However, molecular cellular mechanisms actions poorly known. In study we examined possible involvement mitogen-activated protein kinase (MAPK) pathway in effects. induced phosphorylation MAPK [p44 extracellular signal-regulated 1 (ERK1) p42 ERK2] Swiss 3T3 3T3-F442A fibroblasts but not 3T3-L1 some other lines such as L6E9...
Here we characterize the molecular and biological requirements for OCT4 plasticity induction in human skin derived fibroblasts (hFibs) that allows direct conversion of cell fate without iPSC formation. Our results indicate adult hFibs not only require but also short-term exposure to reprogramming media (RM) successfully undergo early hematopoietic neural progenitor fates. RM was found be essential this process allowed unique changes global gene expression specific combined effects treatment...
Proinsulin C-peptide has been reported to have some biological activities and be possibly involved in the development of diabetic microangiopathy. In present study, we examined effects on mitogen-activated protein kinase pathway LEII mouse lung capillary endothelial cells. Stimulation cells with increased both p38 (p38MAPK) extracellular signal-regulated (ERK1/2) activity-related site-specific phosphorylation respective kinases a concentration-dependent manner, but failed activate c-Jun...
PI3Ks are key signaling enzymes required for triggering many immunological functions. In B lymphocytes, PI3K is Ag-induced proliferation and robust production of most Ab isotypes. Paradoxically, was found to have a negatively regulatory function regarding class switch recombination, blockade can strongly potentiate IgE switch. this article, we explore the mechanisms unexpected negative IgE. We demonstrate that p110δ selectively regulates in cell-intrinsic manner by controlling germline...
It has been demonstrated that proinsulin C-peptide possesses several biological activities and its specific binding sites are present on the surface of cell membranes. However, molecular cellular mechanisms actions poorly known. In study we examined possible involvement mitogen-activated protein kinase (MAPK) pathway in effects. induced phosphorylation MAPK [p44 extracellular signal-regulated 1 (ERK1) p42 ERK2] Swiss 3T3 3T3-F442A fibroblasts but not 3T3-L1 some other lines such as L6E9...
Abstract Background Proinsulin C‐peptide shows ameliorative effects on diabetic complications, possibly through the production of nitric oxide (NO). On contrary, increased local availability NO and expression endothelial synthase (eNOS) in renal endothelium are shown to be involved progression nephropathy. The aim this study was elucidate effect insulin as a reference eNOS early phase type 1 rat kidney. Methods Type diabetes rats produced by streptozotocin injection some were treated with...
B cell Ag receptors function in both signaling activation of Ag-specific cells and collecting specific for presentation to T lymphocytes. Signaling via PI3K is required BCR-mediated functions; however, the relevant downstream targets are incompletely defined. In this study, we have investigated roles effector molecule Bam32/DAPP1 BCR functions. mouse primary cells, Bam32 was efficient GTPase Rac1 JNK, but not BLNK, phospholipase C gamma2, or calcium responses. Consistent with a role adaptor...
Hepatocyte growth factor (HGF) causes endothelium-dependent vasodilation, but its relation to endothelial nitric oxide synthase (eNOS) activity remains be elucidated. Treatment of bovine aortic cells with HGF increased eNOS within minutes, accompanied by an increase activity-related site-specific phosphorylation eNOS. The was completely abolished pretreatment the a phosphoinositide 3-kinase (PI3K) inhibitor (wortmannin) and transfection dominant-negative Akt, enzyme inhibited wortmannin. In...
Non-invasive immobilization stress causes an increase in the plasma interleukin (IL)-6 level accompanied by increased IL-6 mRNA expression and immunoactivity liver [Biochem. Biophys. Res. Commun. (1997) 238, 707-711]. In present study, using rat primary cultured hepatocytes non-parenchymal cells, effect of norepinephrine (NE) on was determined. hepatocytes, but not when cells were treated with NE. The stimulatory NE inhibited combined use alpha- beta-adrenergic antagonists. also incubation...
Abstract Cell migration is controlled by PI3Ks, which generate lipid messengers phosphatidylinositol-3,4,5-trisphosphate and phosphatidylinositol-3,4-bisphosphate [PI(3,4)P2] consequently recruit pleckstrin homology (PH) domain–containing signaling proteins. PI3K inhibition impairs of normal transformed B cells, an effect thought to partly underlie the therapeutic efficacy inhibitors in treatment cell malignancies such as chronic lymphocytic leukemia. Although a number studies have...
The proliferation of peripheral blood mononuclear cells (PBMC) containing both monocyte/macrophages and T lymphocytes increased after treatment with T-cell mitogen (concanavalin A: Con A). PBMC treated either leptin alone or combination ConA showed enhanced proliferative activity by 10-40%, compared those alone. In contrast, isolated lowered than the ConA-treated alone, indicating that induced production some cytokines from monocyte/macrophages, subsequently resulted in enhancement PBMC....
Interleukin (IL)-1β mRNA expression in the liver and spleen was examined after subjection to oscillation stress rat. Thirty-minute increased IL-1β both organs. Prior treatment of rats with gadolinium chloride, which eliminates macrophages, prevented stress-induced expression. Either adrenalectomy or guanethidine, a blocker norepinephrine release sympathetic nerve endings, partially attenuated response, but combined completely blocked it. Injection β-adrenergic antagonist (propranolol) also...
Leptin, a pleiotropic hormone regulating food intake and energy expenditure, has been shown to directly modulate human polymorphonuclear neutrophil (PMN) functions or indirectly through the action of tumor necrosis factor-α (TNF-α). Bovine PMN have considerable different characteristics from PMN. For example, it does not respond N-formyl-Methionyl-Leucyl-phenylalanine, well known activator. In present study, we tested effects leptin TNF-α on superoxide production degranulation bovine...
Glanders is a highly contagious and life-threatening zoonotic disease caused by Burkholderia mallei (B. mallei). Without an effective vaccine or treatment, early diagnosis has been regarded as the most method to prevent glanders transmission. Currently, of heavily reliant on serological tests. However, given that markedly different host immune responses can be elicited genetically strains same bacterial species, infection B. mallei, whose genome unstable plastic, may result in various...