A5048270675- Scientific Computing and Data Management
- Research Data Management Practices
- Biomedical Text Mining and Ontologies
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Cell Image Analysis Techniques
- Social Capital and Networks
- Genetics, Bioinformatics, and Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Protease and Inhibitor Mechanisms
- Single-cell and spatial transcriptomics
- Cellular Mechanics and Interactions
- Mitochondrial Function and Pathology
- Scientific Research and Discoveries
- Wnt/β-catenin signaling in development and cancer
- Protein Tyrosine Phosphatases
- Biomedical and Engineering Education
- Liver physiology and pathology
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Big Data Technologies and Applications
- Invertebrate Immune Response Mechanisms
Film Independent
2023
OKF (Czechia)
2020
Open Knowledge (United Kingdom)
2019
Oregon Health & Science University
2017-2018
Center for Neurosciences
2017
Emory University
2004-2007
In many disciplines, data are highly decentralized across thousands of online databases (repositories, registries, and knowledgebases). Wringing value from such depends on the discipline science humble bricks mortar that make integration possible; identifiers a core component this infrastructure. Drawing our experience work by other groups, we outline 10 lessons have learned about identifier qualities best practices facilitate large-scale integration. Specifically, propose actions...
Annexin 1 (AnxA1) is a multifunctional phospholipid-binding protein associated with the development of metastasis in some invasive epithelial malignancies. However, role AnxA1 migration/invasion cells not known. In this study, experiments were performed to investigate invasion model cell line, SKCO-15, derived from colorectal adenocarcinoma. Small interfering RNA-mediated knockdown expression resulted significant reduction through Matrigel-coated filters. Localization studies revealed...
Migration of epithelial cell sheets, a process involving F-actin restructuring through Rho family GTPases, is both physiologically and pathophysiologically important. Our objective was to clarify the mechanisms whereby downstream RhoA effector Rho-associated coil-coil-forming kinase (ROCK) influences coordinated motility. Although cells exposed pharmacological ROCK inhibitor (Y-27632) exhibited increased spreading in wound closure assays, they failed migrate cohesive manner. Two main...
Data are the foundation of science, and there is an increasing focus on how data can be reused enhanced to drive scientific discoveries. However, most seemingly "open data" do not provide legal permissions for reuse redistribution. The inability integrate redistribute our collective resources blocks innovation stymies creation life-improving diagnostic drug selection tools. To help biomedical research support communities (e.g. libraries, funders, repositories, etc.) understand navigate...
Abstract Neuronal damage induced by injury, stroke, or neurodegenerative disease elicits swift immune responses from glial cells, including altered gene expression, directed migration to injury sites, and clearance of damaged neurons through phagocytic engulfment. Collectively, these hinder further cellular damage, but the mechanisms that underlie important protective reactions are still unclear. Here, we show evolutionarily conserved trimeric protein phosphatase 4 (PP4) serine/threonine...
Superficial wounds in the gastrointestinal tract rapidly reseal by coordinated epithelial cell migration facilitated cytokines such as hepatocyte growth factor (HGF)/scatter released wound vicinity. However, mechanisms which HGF promotes physiological and pathophysiologic are incompletely understood. Using vitro models of polarized T84 Caco-2 intestinal epithelia, we report that promoted spreading RhoA GTPase activation a time-dependent manner. Inducible expression enhanced green fluorescent...
ABSTRACT Data is the foundation of science, and there an increasing focus on how data can be reused enhanced to drive scientific discoveries. However, most seemingly “open data” do not provide legal permissions for reuse redistribution. Not being able integrate redistribute our collective resources blocks innovation, stymies creation life-improving diagnostic drug selection tools. To help biomedical research support communities (e.g. libraries, funders, repositories, etc.) understand...
Abstract In many disciplines, data is highly decentralized across thousands of online databases (repositories, registries, and knowledgebases). Wringing value from such depends on the discipline science humble bricks mortar that make integration possible; identifiers are a core component this infrastructure. Drawing our experience work by other groups, we outline ten lessons have learned about identifier qualities best practices facilitate large-scale integration. Specifically, propose...
The Community of Open Scholarship Grassroots Networks (COSGN), includes 120 grassroots networks, representing virtually every region the world and research discipline. These networks communicate coordinate on topics common interest. We propose, using an NSF 19-501 Full-Scale implementation grant, to formalize governance coordination maximize impact establish standard practices for sustainability. In project period, we will increase capacity COSGN advance community goals participating...
Understanding normal and aberrant in vivo cell behaviors is necessary to develop clinical interventions thwart disease initiation progression. It therefore critical optimize imaging approaches that facilitate the observation of dynamics situ, where tissue structure composition remain unperturbed. The epidermis body's outermost barrier as well source most prevalent human cancers, namely cutaneous skin carcinomas. accessibility presents a unique opportunity monitor epithelial dermal intact...
Understanding normal and aberrant in vivo cell behaviors is necessary to develop clinical interventions thwart disease initiation progression. It therefore critical optimize imaging approaches that facilitate the observation of dynamics situ, where tissue structure composition remain unperturbed. The epidermis body's outermost barrier, as well source most prevalent human cancers, namely cutaneous skin carcinomas. accessibility presents a unique opportunity monitor epithelial dermal intact...
Annexin 1 (AnxA1) is a multifunctional phospholipid binding protein that has been identified as metastasis-associated in subset of epithelial malignancies. We hypothesize AnxA1 regulates cell migration/invasion. Mechanisms by which annexin motility are unknown. To analyze the role regulating migration, we used model line (SKCO-15) derived from colorectal adenocarcinoma. Localization studies revealed presence on surface SKCO-15 cells was increased upon induction migration. Functionally,...