A5060598058- Enzyme Structure and Function
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Protein Structure and Dynamics
- Advanced X-ray Imaging Techniques
- Metal complexes synthesis and properties
- Carbohydrate Chemistry and Synthesis
- Magnetism in coordination complexes
- Crystallography and molecular interactions
- Advanced Electron Microscopy Techniques and Applications
- Biochemical and Molecular Research
- Photosynthetic Processes and Mechanisms
- Crystal structures of chemical compounds
- Metal-Catalyzed Oxygenation Mechanisms
- Medical Imaging Techniques and Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Educational Methods and Media Use
- Enzyme Production and Characterization
- Molecular spectroscopy and chirality
- Radioactive contamination and transfer
- Inorganic Fluorides and Related Compounds
- Fluorine in Organic Chemistry
- Radiation Therapy and Dosimetry
- Porphyrin Metabolism and Disorders
- Nuclear Physics and Applications
Hermann Buhl Institut
2023
Deutsches Elektronen-Synchrotron DESY
2011-2021
Friedrich Schiller University Jena
2006-2013
University of Oslo
2012
University of Gothenburg
2012
Lawrence Berkeley National Laboratory
2011
Stockholm University
2009-2011
Carl Zeiss (Germany)
2006-2008
A new approach for collecting data from many hundreds of thousands microcrystals using X-ray pulses a free-electron laser has recently been developed. Referred to as serial crystallography, diffraction patterns are recorded at constant rate suspension protein crystals flows across the path an beam. Events that by chance contain single-crystal retained, then indexed and merged form three-dimensional set reflection intensities structure determination. This relies upon several innovations:...
Multilayer Laue lenses are volume diffraction elements for the efficient focusing of X-rays. With a new manufacturing technique that we introduced, it is possible to fabricate sufficiently high numerical aperture (NA) achieve focal spot sizes below 10 nm. The alternating layers materials form lens must span broad range thicknesses on nanometer scale necessary X-ray deflection angles required NA. This poses challenge both accuracy deposition process and control properties, which often vary...
Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial temporal resolution remains a major challenge in structural biology. The development serial crystallography methods X-ray free-electron lasers subsequently synchrotron light sources allows new approaches to tackle this challenge. Here, polyimide tape drive designed for mix-and-diffuse experiments is reported. structure lysozyme bound by competitive inhibitor chitotriose was determined using...
Recent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback synchrotron sources as well. With crystal lifetimes typically in the millisecond range and latest-generation detector technologies with high framing rates up 1 kHz, fast sample exchange become bottleneck for such experiments. A micro-patterned chip been developed from single-crystalline silicon, which acts holder several thousand microcrystals very low background level. The crystals...
Efficient and reliable sample delivery has remained one of the bottlenecks for serial crystallography experiments. Compared with other methods, fixed-target offers advantage significantly reduced consumption shorter data collection times owing to higher hit rates. Here, a new method on-chip crystallization is reported which allows efficient reproducible growth large numbers protein crystals directly on micro-patterned silicon chips in-situ Crystals are grown by sitting-drop vapor diffusion...
Per- and polyfluoroalkyl substances (PFAS) are contaminants of emerging concern, as they persistent, ubiquitous, toxic. They pose a threat to both human health the environment, therefore efficient remediation strategies urgently needed. One possible technology treat contaminated soil is thermal desorption. However, chemical processes potential transformation products created during desorption have not been fully assessed. Especially precursor substances, that transform persistent PFAS in...
The sugar-modified Schiff base ligand benzyl 2-deoxy-2-salicylideneamino-α-d-glucopyranoside H2L, prepared by condensation of salicylaldehyde and the monomeric chitosan analogue 2-deoxy-2-amino-α-d-glucopyranoside, reacts with copper(II) acetate to form a self-assembled, alkoxo-bridged tetranuclear homoleptic complex [{Cu(L)}4] (4) Cu4O4 heterocubane core. chiral 4 crystallizes in space group P212121. is composed two dinuclear {Cu(L)}2 entities linked four μ3-bridging C-3 alkoxide oxygen...
The trinuclear copper(ii) complex [Cu3(saltag)(py)6]ClO4 (H5saltag = tris(2-hydroxybenzylidene)triaminoguanidine) was synthesized and characterized by experimental as well theoretical methods. This exhibits a strong antiferromagnetic coupling (J -298 cm-1) between the ions, mediated N-N diazine bridges of tritopic ligand, leading to spin-frustrated system. compound shows T2 coherence time 340 ns in frozen pyridine solution, which extends 591 changing solvent pyridine-d5. Hence, presented is...
Accurate in vivo localisation of minimal amounts functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal spatial resolution, large depth penetration, arbitrarily long serial measurements. X-ray fluorescence could offer such capabilities; however, its utilisation for human-sized scales is hampered by intrinsic background level. Here we measure model this...
The reaction of benzyl 2-amino-4,6-O-benzylidene-2-deoxy-alpha-D-glucopyranoside (HL) with the metal salts Cu(ClO(4))(2)6 H(2)O and Ni(NO(3))(2)6 affords via self-assembly a tetranuclear mu(4)-hydroxido bridged copper(II) complex [(mu(4)-OH)Cu(4)(L)(4)(MeOH)(3)(H(2)O)](ClO(4))(3) (1) trinuclear alcoholate nickel(II) [Ni(3)(L)(5)(HL)]NO(3) (2), respectively. Both complexes crystallize in acentric space group P2(1). X-ray crystal structure reveals rare (mu(4)-OH)Cu(4)O(4) core for 1 which is...
High-pressure freezing (HPF) is a method which allows sample vitrification without cryoprotectants. In the present work, protein crystals were cooled to cryogenic temperatures at pressure of 210 MPa. contrast other HPF methods published date in field cryocrystallography, this protocol involves rapid cooling using standard device. The fast rates allow directly their mother liquor need for cryoprotectants or external reagents. was first attempted with hen egg-white lysozyme and cubic insulin...
Beamline P11 at PETRA III is dedicated to structural investigations of biological samples. It provides two experimental stations, one for macromolecular crystallography and X-ray microscopy. The microscope will provide full field Zernike phase contrast scanning microscopy both in 2D tomographic mode. Full with a view 50 x μm<sup>2</sup> allow generate an overview the sample select regions interest later inspection element distribution by fluorescence diffraction Central part inhouse...
Abstract Condensation of a derivatized 2‐aminoglucose fragment with salicylaldehyde affords the new sugar‐based Schiff base ligand benzyl 4,6‐ O ‐benzylidene‐2‐deoxy‐2‐salicylideneamino‐α‐ D ‐glucopyranoside (H 2 L). The reaction dibasic H L [Cu(CH 3 COO) ] · leads to formation tetranuclear copper(II) complex [{Cu(L)} 4 ( ) by self‐assembly process. X‐ray structural analysis which crystallizes together two molecules chloroform and one molecule ethanol in space group P 1 revealed for all...
A coherent x-ray diffraction experiment was performed on an isolated colloidal crystal grain at the coherence beamline P10 PETRA III.Using azimuthal rotation scans three-dimensional (3D) scattered intensity in reciprocal space from sample measured.It includes several Bragg peaks as well interference around these peaks.The analysis of reveals presence a plane defect single sample.We confirm findings by model simulations.In simulations we also analyze experimental conditions to phase 3D...
Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which internalized differently functionalized gold nanoparticles. Total order few hundred picograms could be conveniently observed microsamples consisting only hundreds cells. A comparison mass spectroscopy...
Trifluorinated germanium anions attracted attention of theoretical chemists already in the late 1990s to predict their physical and chemical properties. However these species were not synthesized laboratory, although substantial evidence for existence was obtained from mass spectrometry GeF4. The present study shows that controlled fluorination LMNMe2 (L = PhC(N(t)Bu)2, M Ge, Sn) using HF·pyridine toluene leads formation [LH2](+)[MF3](-) under elimination HNMe2. products contain...
Specific radiation damage can be used to determine phases de novo from macromolecular crystals. This method is known as radiation-damage-induced phasing (RIP). One limitation of the that dose individual data sets must minimized, which in turn leads with low multiplicity. A solution this problem use multiple However, resulting signal degraded by a lack isomorphism between Here, it shown serial synchrotron crystallography combination selective merging high-quality for insulin and thaumatin,...
Successful cryogenic X-ray structure determination from a single high-pressure-frozen bovine enterovirus 2 crystal is reported. The presented high-pressure-freezing procedure based on commercially available device and allows the cryocooling of macromolecular crystals directly in their mother liquor without time- crystal-consuming search for optimal cryoconditions. method generally applicable will allow data collection all types crystals.
The sugar-modified Schiff-base ligands derived from benzyl 2-deoxy-2-salicylideneamino-α-D-glucopyranoside (H2L5-Br and H2L3-OMe) were used to prepare the chiral oxidovanadium(V) complexes [VO(L5-Br)(OMe)] (1) [VO(L3-OMe)(OMe)] (2) which can be isolated a methanol solution as six-coordinate with an additional ligand [VO(L5-Br)(OMe)(MeOH)] (1–MeOH) [VO(L3-OMe)(OMe) (MeOH)] (2–MeOH). Both crystallize in orthorhombic space group P212121 together two solvent molecules of 1–MeOH·2MeOH...