A5062043414- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Polyomavirus and related diseases
- Fire effects on ecosystems
- HIV Research and Treatment
- Vibrio bacteria research studies
- Biological Research and Disease Studies
- Viral Infections and Vectors
- Full-Duplex Wireless Communications
- Hepatitis Viruses Studies and Epidemiology
- Chronic Lymphocytic Leukemia Research
- Carcinogens and Genotoxicity Assessment
- Alcohol Consumption and Health Effects
- Infection Control and Ventilation
- Salmonella and Campylobacter epidemiology
- Mosquito-borne diseases and control
- Advanced Chemical Sensor Technologies
- Plant Virus Research Studies
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
Rega Institute for Medical Research
2005-2014
KU Leuven
2005-2014
The prior diagnosis of fatal astrocytoma in a 60-year-old man with Crohn's disease treated natalizumab, monoclonal antibody against alpha4 integrins, was reclassified as JC virus-related progressive multifocal leukoencephalopathy (PML). Analysis frozen serum samples showed that virus DNA had appeared the three months after initiation open-label natalizumab monotherapy and two before appearance symptomatic PML. There staining brain lesion for polyomavirus. This case report, along others,...
ABSTRACT Until recently, human coronaviruses (HCoVs), such as HCoV strain OC43 (HCoV-OC43), were mainly known to cause 15 30% of mild upper respiratory tract infections. In recent years, the identification new HCoVs, including severe acute syndrome coronavirus, revealed that HCoVs can be highly pathogenic and more lower infections, bronchiolitis pneumonia. To date, no specific antiviral drugs prevent or treat infections are available. We demonstrate chloroquine, a widely used drug with...
Hepatitis C virus (HCV) genotyping is a tool used to optimize antiviral treatment regimens. The newly developed Versant HCV genotype assay (LiPA) 2.0 uses sequence information from both the 5' untranslated region and core region, allowing distinction between 1 subtypes c l of 6 b 1. HCV-positive samples were genotyped manually using system according manufacturer's instructions. For comparison study, 1.0 was used. In this 99.7% could be amplified, 96.0% determined, agreement with reference...
ABSTRACT Epidemiological and phylogenetic studies of hepatitis C virus (HCV) have identified six major HCV genotypes attempted to characterize their origin spread worldwide. Putative regions endemic infection been for all except genotype 5a. Although 5a was previously thought be largely restricted the northern part South Africa, this study reports an unexpected cluster in West Flanders Province Belgium. To investigate molecular epidemiology general, a rigorous analysis Belgian African...
<h3>Background and aims:</h3> Anti-α4 integrin therapy with natalizumab is efficacious in refractory Crohn's disease multiple sclerosis, but carries an estimated 1/1000 risk of progressive multifocal leukoencephalopathy (PML) caused by reactivation latent JC virus infection. Athough anti-α4 therapies are likely to be introduced the clinic, screening for PML has not been developed. <h3>Methods:</h3> We prospectively collected urine, serum, plasma buffy coats from 125 patients disease, 100...
Molecular evolutionary patterns of 62 HBV full-length genomes obtained from Belgian patients were characterized. Phylogenetic analysis revealed diverse subgenotypes including A2 and A6 (46.8%), D1-D4 (38.8%), E (9.7%), C1 (1.6%), B2 (1.6%). The study population consisted with different ethnic origin (Caucasian, Turkish, Asian, Arab, African). One D/C recombinant isolate was identified, which encoded subtype adw2. An subgenotype D4 an aberrant ayw4 detected. Although none the genotypes...
Exhaled breath condensate (EBC) sampling has been considered an inventive and novel method for the isolation of respiratory viruses. In our study, 102 volunteers experiencing upper airway infection were recruited over winter early spring 2008/2009 first half 2009/2010. Ninety-nine EBCs successfully obtained screened 14 commonly circulating To investigate efficiency virus from EBC, a nasal swab was taken in parallel subset volunteers. The combined use ECoVent device with RTube™ allowed...
Abstract Hepatitis B virus (HBV) and human immunodeficiency (HIV) have similar transmission routes, implying that patients infected with HIV are at particular risk for HBV infection. Patients who co‐infected progress more rapidly to end‐stage liver disease different genotypes may a distinct impact on progression. One hundred ninety‐one anti‐HBc‐positive sera from Belgian were collected during 1998–2008. Full‐length genomes as well large S or partial genes amplified their molecular...
The genome of the hepatitis C virus (HCV) exhibits a high genetic variability. This remarkable heterogeneity is mainly attributed to gradual accumulation mutational changes, whereas contribution recombination events evolution HCV remains controversial so far. While performing phylogenetic analyses including large number sequences deposited in GenBank, we encountered full-length sequence (AY651061) that showed evidence for inter-subtype and was, therefore, subjected detailed analysis its...
ABSTRACT Since auditory disability causes serious problems in the development of speech and total a child, it is crucial to diagnose possible hearing impairment as soon after birth. This study evaluates neonatal screening program Flanders, Belgium. The ability 118,438 babies was tested using automated brainstem response. We selected 194 with indicative 332 matched controls investigate association between presence human cytomegalovirus (HCMV) urine samples sensorineural loss analyze...
ABSTRACT To validate a commercially available line probe assay for samples containing the infrequently found hepatitis C virus (HCV) genotype 5a, we sequenced 511-nucleotide fragment of NS4b region Belgian and South African HCV 5a samples. Phylogenetic analysis sequence data was performed. For 77 collected, there 100% concordance between assignment by genotyping based on nucleotide sequencing, despite heterogeneity in binding sites some
A 32-base pair deletion in the CC-chemokine receptor 5 gene (CCR5), associated with resistance to human immunodeficiency virus type 1 (HIV-1) infection, has recently been suggested act as an adverse host factor hepatitis C (HCV) infection. To examine this hypothesis, we determined CCR5-Δ32 allele frequency by polymerase chain reaction a Belgian cohort of 163 HCV-infected patients and 310 healthy control subjects. The resulting frequencies were 0.080 0.119 for patient group group,...
Abstract HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of genome replication, quantitative strand‐specific real‐time RT‐PCR assays were developed and applied to livers explanted because end‐stage cirrhosis. The have broad ranges determination a high reproducibility accuracy. Analysis five different samples showed even distribution genomes four livers. Hepatic concentrations positive (PS)‐ (NS)‐strand RNA did correlate with each other, PS/NS ratios...