Abstract Background Tendon stem cells (TSCs) have been reported to hold promises for tendon repair and regeneration. However, less is known about the effects of exosomes derived from TSCs. Therefore, we aimed clarify healing TSC-derived (TSC-Exos) on injury. Methods The Achilles tendons Sprague-Dawley male rats were used primary culture TSCs tenocytes, isolated proliferation tenocytes induced by TSC-Exos was analyzed using an EdU assay; cell migration measured scratch transwell assays. We...

The use of adipose-derived mesenchymal stromal cell-derived exosomes (ADSC-Exos) may become a new therapeutic method in biomedicine owing to their important role regenerative medicine. However, the ADSC-Exos tendon repair has not yet been evaluated. Therefore, we aimed clarify healing effects on injury.The cells (ADSCs) and stem (TSCs) were isolated from subcutaneous fat tissues Sprague-Dawley rats, respectively, ADSCs. proliferation migration TSCs induced by analyzed EdU, cell scratch,...

Rice bran protein hydrolysates (RBPH) pretreated with high hydrostatic pressure (HHP) covalently interacted ferulic acid (FA) (0.5 to 2.5 mg/mL) under alkaline conditions. The structural and functional properties of the conjugates were investigated. results revealed that FA binding equivalent on RBPH increased from 6.03 207.64 nmol/mg. FTIR spectral analysis indicated content α-helix increased, whereas contents β-sheet, β-turn, random coil decreased. surface hydrophobicity (H0) fluorescence...

In this study, albumin and globulin were separated from rice bran hydrolyzed with trypsin. The fractions of these proteins their hydrolysates comparatively studied to understand the mechanisms functional changes. SDS–PAGE showed that molecular weights two decreased. addition, number α-helices random coils increased, which was consistent structure observed by SEM. Oil-in-water emulsions prepared hydrolysate globulin, ability resist environmental pressure (pH: 3–9; salt addition: 0–300 mM NaCl...

Nonfused ring electron acceptors (NFREAs) have become a research hotspot of organic solar cells (OSCs) due to their facile synthesis. However, efficient NFREAs not only need maintain the advantages FREAs but also optimize molecular structure conjugate backbone achieve good planarity. Therefore, choosing appropriate building blocks is prerequisite for achieving OSCs. Here, two simple 2T2CSi-4F and 4T2CSi-4F, based on diester-thieno[3,2-b]thiophene (2T2C) as central core unit...

Breast cancer, which is the most common malignant tumor among women worldwide and an important cause of death in women. The existing prognostic model for patients with breast cancer not accurate as resistant to commonly used antitumor drugs. Ferroptosis a novel mechanism programmed cell that depends on iron accumulation lipid peroxidation. Various studies have confirmed role ferroptosis regulation now considered play development. At present, association between prognosis ferroptosis-related...

A total of 24,000 healthy 1-day-old Arbor Acres broilers with similar initial weights were used in this study and fed a basal diet supplemented 0, 400 800 mg/kg isoleucine (Ile), denoted CON, ILE400 ILE800, respectively. Results revealed that the final body weight, average daily weight gain, eviscerated carcass rate, broiler chickens group significantly higher than other groups (p < 0.05). In addition, ILE800 had lower feed conversion rate survival breast muscle 0.05), while abdominal fat...

Tendon repair is a medical challenge. Our present study investigated the effectiveness of acellular therapy consisting conditioned medium (CM) tendon stem cells (TSCs) induced with hepatocyte growth factor (HGF) in promoting healing injured Achilles rat model. Proteomic analysis soluble substances CM was performed using an array chip, and bioinformatic carried out to evaluate interactions among factors. The effects on viability migratory capacity fibroblasts derived from rats ruptured were...

Adipose mesenchymal stromal cell-derived exosomes (ADSC-Exos) have shown great potential in the treatment of oxidative stress induced by ischemia-reperfusion injury. However, alleviation testicular torsion injury ADSC-Exos has not been reported. Therefore, we investigated protective effect against torsion-detorsion were isolated ultracentrifugation and injected into torsion-detorsion-affected testes rats. H&amp;E staining sperm quality used to evaluate therapeutic effects ADSC-Exos, tissue...

Peritoneal regeneration and repair can alleviate postoperative intraperitoneal adhesions, mesenchymal stem cells (MSCs) have demonstrated the potential for peritoneal regeneration. However, extracellular vesicles (EVs) are main carriers MSC activity. Thus far, roles of MSC-derived EVs on not well understood. To investigate therapeutic effect adipose-derived cell-derived (ADSC-EVs) in injuries, ADSC-EVs were injected vivo via tail vein rats. The antiadhesion effects evaluated following...

Improving the grain yield of rice is a central goal basic and applied scientific research. Here, we identified an anion transporter, OsAT1, localized in endoplasmic reticulum Golgi. OsAT1 highly expressed flag, stem, sheath as monitored using qRT-PCR pOsAT1::GUS. Thousand-grain weight, weight per plant, content starch were significantly increased knock-down mutants (OsAT1-Ri) but decreased overexpressed lines (OsAT1-OE). In addition, plant by 6.17% to 6.78% OsAT1-RNAi lines, whereas it...

Background: Fibrosis and inflammation due to ureteropelvic junction obstruction substantially contributes poor renal function. Urine-derived stem-cell-derived exosomes (USC-Exos) have therapeutic effects through paracrine. Methods: In vitro, the of USC-Exos on biological functions HK-2 human umbilical vein endothelial cells were tested. Cell fibrosis induced by transforming growth factor-β1 interleukin-1β, their anti-inflammatory antifibrotic observed after exogenous addition USC-Exos....

Abstract Background Wilms’ tumor (WT) is the most common renal in childhood. Pyroptosis, a type of inflammation-characterized and immune-related programmed cell death, has been extensively studied multiple tumors. In current study, we aim to construct pyroptosis-related gene signature for predicting prognosis tumor. Methods We acquired RNA-seq data from TARGET kidney projects constructing signature, snRNA-seq GEO database validating signature-constructing genes. Pyroptosis-related genes...

Necroptosis is a novel programmed cell death pathway proposed in 2005, which mainly activated by the tumor necrosis factor (TNF) family and mediates cellular disassembly via receptor interacting serine/threonine kinase 1 (RIPK1), 3 (RIPK3) mixed lineage domain like pseudokinase (MLKL). We tried to analyze relationship of necroptosis-related genes (NRGs) expression with colon adenocarcinoma (COAD) propose potential therapeutic targets through immunological analysis.First, we evaluated NRGs...

Wilms tumor (WT) commonly occurs in infants and children. We evaluated clinical factors the expression of multiple RNAs WT samples TARGET database. Eight long non-coding (lncRNAs; AC079310.1, MYCNOS, LINC00271, AL445228.3, Z84485.1, AC091180.5, AP002518.2, AC007879.3), two microRNAs (miRNAs; hsa-mir-152 andhsa-mir-181a), nine messenger (mRNAs; TCTEX1D4, RNF133, VRK1, CCNE1, HEY1, C10orf71, SPRY1, SPAG11A, MAGEB18) were screened from differentially expressed used to construct predictive...

Aimed at the Problems of water bloom erupting often in cities our country, a Pre-Warning Model for is developed by using ANN method; order to adopt emergency response measures and avoid bloom, provide scientific basis improving environment river lake system. The results research indicates: (1) system dynamic condition main factor causing change best way avoiding eruption bloom. (2) present concentration Chl-a pre-warning index, if has erupted, measure allocation may relax tension so far as...

The therapeutic impact of stem cells is potentially largely attributable to secretion exosomes and soluble factors. present study evaluates the hepatocyte growth factor (HGF)–expressing tendon (TSCs) on healing in a rat model. Patellar TSCs were isolated underwent transfection with lentiviral vectors containing HGF or green fluorescent protein (GFP) genes. In vivo , immunohistochemistry tendons sampled 1 week postsurgery demonstrated that all cell–treated groups exhibited higher numbers...

Wilms tumor (WT) is a widespread urologic in children. Ferroptosis, on the other hand, novel form of cell death associated with development. In this study, we aim to explore predictability ferroptosis-related biomarkers estimating prognosis WT patients.To determine link between gene expression and prognosis, first collected RNA sequencing data clinical information, involving 124 6 healthy tissue samples, from TARGET database. Next, screened information for long non-coding using Cox...

Clear cell renal carcinoma (ccRCC) is one of the most common carcinomas worldwide, which has worse prognosis compared with other subtypes tumors. We propose a potential RNA regulatory mechanism associated ccRCC progression. Accordingly, we screened out clinical factors and expression RNAs miRNAs from TCGA database. 9 lncRNAs (FGF12-AS2, WT1-AS, TRIM36-IT1, AC009093.1, LINC00443, TCL6, COL18A1-AS1, AC110619.1, HOTTIP), 2 (mir-155 mir-21), 3 mRNAs (COL4A4, ERMP1, PRELID2) were selected...