The genome of the porcine transmissible gastroenteritis coronavirus is a plus-strand, polyadenylylated, infectious RNA molecule approximately 20 kilobases. During virus replication, seven subgenomic mRNAs are generated by what thought to be leader-priming mechanism form 3'-coterminal nested set. By using radiolabeled, strand-specific, synthetic oligodeoxynucleotide probes in blot hybridization analyses, we have found minus-strand counterpart for and each mRNA species cytoplasm infected...

The present investigation examined whether an association exists between betel quid chewing and signs of periodontal disease determined the prevalence Actinobacillus actinomycetemcomitans Porphyromonas gingivalis by polymerase chain reaction. status 34 chewers 32 non‐betel were compared. A significantly higher bleeding on probing was found in than non‐chewers among subjects with plaque level, greater gingival inflammation, deeper depth or attachment loss. Also, results suggested that may...

Glycoproteins C (gC) from herpes simplex virus type 1 (HSV-1) and HSV-2, gC-1 gC-2, bind the human complement fragment C3b, although two glycoproteins differ in their abilities to act as C3b receptors on infected cells effects alternative pathway. Previously, we identified three regions of gC-2 (I, II, III) which are important for binding. In this study, our goal was identify C3b-binding sites continue analysis gC-2. We constructed a large panel mutants by using cloned genes. Most mutant...

Herpesviruses may play roles in the development of periodontal diseases. The present study evaluated relationships between herpesviruses and severity periodontitis.Periodontal status terms gingival inflammation, bleeding on probing, probing depth, clinical attachment loss 20 participants was evaluated. presence herpes simplex virus (HSV), human cytomegalovirus (HCMV), or Epstein-Barr type 1 (EBV-1) six subgingival plaques from each participant examined by nested-polymerase chain reaction...

Glycoprotein C (gC) of herpes simplex virus type 1 (HSV-1) or 2 (HSV-2) binds the human complement fragment C3b, but two proteins differ in their ability to bind C3b on infected cell surfaces. In addition, gC-1, not gC-2, accelerates decay alternative pathway C3 convertase, thereby affecting later steps cascade. Previously, we constructed linker insertion and deletion mutants gC-1 gC-2 used transient transfection express mutant uninfected cells. spite differences between binding was...

Porphyromonas gingivalis is a major periodontal pathogen that contains variety of virulence factors. The antibody titer to P. GroEL, homologue HSP60, significantly higher in periodontitis patients than healthy control subjects, suggesting GroEL potential stimulator disease. However, the specific role disease remains unclear. Here, we investigated effect on human ligament (PDL) cells vitro, as well its alveolar bone resorption rats vivo. First, found stimulation PDL with recombinant increased...

The aim of the present study was to evaluate influence membrane exposure on guided tissue regeneration (GTR). Thirty patients with 2‐wall or 3‐wall intraosseous defects were treated GTR. Periodontal index, gingival bleeding probing, probing depth, clinical attachment level and recession assessed at baseline examination, as well immediately prior six months after numbers Porphyromonas gingivalis Actinobacillus actinomycetemcomitans retrieved expanded polytetrafluoroethylene (e‐PTFE) membranes...

Cytotoxic effects of resin liquids three in situ relining dental polymers, Alike, Kooliner, and Tokuso Rebase, their major components, methyl methacrylate (MMA), isobutyl (IBMA), 1,6-hexanediol dimethacrylate (1,6-HDMA) were investigated. The concentrations monomers these determined by high-performance liquid chromatography. Cellular viability human gingival fibroblasts (GF) periodontal ligament (PDL) cells evaluated the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide assay....

Abstract Collagen sponges are widely used scaffolds in bone engineering. To form bone, the osteoblastic cells undergo proliferation, differentiation, and mineralization stages scaffold. Crosslinking freezing temperature two important variables fabricating collagen sponges. The purpose of this study was to examine responses prepared with or without glutaraldehyde crosslinking at different temperatures (−20°C −80°C). MC3T3‐E1 were cultured differently Osteoblastic examined included cell...

This study compared bacterial penetration through guided tissue regeneration (GTR) membranes impregnated with antibiotics.Three barrier membranes, expanded polytetrafluoroethylene (ePTFE) membrane, collagen and glycolide fiber composite were loaded amoxicillin or tetracycline. The of Streptococcus mutans Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) the GTR was achieved using a device consisting an inner tube outer bottle filled culture media.The S....

Microbial colonization on barrier materials used in guided tissue regeneration (GTR) may adversely affect treatment outcomes. The purposes of this study were: 1) to compare the invasion Streptococcus mutans and Actinobacillus actinomycetemcomitans through 3 GTR membranes, composed expanded polytetrafluoroethylene (ePTFE; non-resorbable), a glycolide fiber composite, type I collagen (both bioabsorbable), 2) explore effects bacteria attachment periodontal ligament (PDL) fibroblasts onto these...

Aqueous e xtracts of ripe a reca n ut without husk (ripe ANE) and fresh tender areca nut with (tender were examined for their effects on the defensive functions human neutrophils. Exposure peripheral blood neutrophils to ANE inhibited bactericidal activity against oral pathogens, including Actinobacillus actinomycetemcomitans Streptococcus mutans , in dose‐dependent manner. At concentrations tested, ANEs did not significantly affect viability as verified by ability exclude trypan blue dye....

Background: Hyperglycemia is widely considered to be the causal link between diabetes mellitus (DM) and diabetic complications. The purpose of this study determine effects high glucose in presence lipopolysaccharide (LPS) purified from periodontal pathogen Porphyromonas gingivalis on human macrophages. Methods: Macrophages (U937) were treated with various concentrations P. –LPS under normal (5.5 mM) or (25 conditions. Mitochondrial dehydrogenase activity was determined using...

Background: Early exposure of a guided tissue regeneration (GTR) membrane in the oral cavity results bacterial contamination, which may lead to failure or incomplete regeneration. Incorporation antimicrobial agents GTR membranes be valuable control membrane‐associated infection during therapy. The purpose this study was evaluate whether incorporation chlorhexidine into various improves attachment periodontal ligament cells presence Actinobacillus actinomycetemcomitans . Methods: possible...

A higher prevalence of periodontal diseases among areca chewers than non-areca has been demonstrated. Neutrophils, representing the first line host defense mechanism against microbial infection, play important roles in maintaining health. This study determined possible effects nut on phagocytosis, chemotaxis, and adhesion human neutrophils.Aqueous extracts ripe without husk (rANE) fresh tender with (tANE) were examined for their neutrophil phagocytosis using flow cytometry confocal laser...

Herpes simplex virus type 1 (HSV-1) is the pathogenic agent of human diseases, including gingivostomatitis and herpes labialis. The anti-viral activities tea polyphenol, epigallocatechin-3-gallate (EGCG), have been demonstrated. This study examined combined effects EGCG antiviral drug, acyclovir (ACV), on infection HSV-1 in oral epithelial cells.Cell viability was using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide. Viral yields were determined plaque assay. proteins detected...