A5040949816- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Aging and Gerontology Research
- Virology and Viral Diseases
- Malaria Research and Control
- Vector-borne infectious diseases
- Plant Virus Research Studies
- Retirement, Disability, and Employment
- Viral Infections and Outbreaks Research
- MRI in cancer diagnosis
- Asthma and respiratory diseases
- Topic Modeling
- Birth, Development, and Health
- CAR-T cell therapy research
- Respiratory viral infections research
- Intergenerational Family Dynamics and Caregiving
- Natural Language Processing Techniques
- Innovations in Medical Education
- T-cell and B-cell Immunology
- Migration, Aging, and Tourism Studies
- Insect symbiosis and bacterial influences
- Health disparities and outcomes
- Virus-based gene therapy research
- Technology Use by Older Adults
- Allergic Rhinitis and Sensitization
National Cheng Kung University
2008-2025
National Tsing Hua University
2023
University of California, San Francisco
2022
Johns Hopkins Medicine
2020
Johns Hopkins University
2020
Tzu Chi University
2002-2016
Hualien Tzu Chi Medical Center
1999-2016
Buddhist Tzu Chi General Hospital
2015
University of California, Irvine
2012-2013
D.F. Chebotarev Institute of Gerontology
2013
Molecularly imprinted film was fabricated in the presence of a pentadecapeptide onto quartz crystal microbalance (QCM) chip. This 15-mer peptide has been known as linear epitope dengue virus NS1 protein. Imprinting resulted an increased polymer affinity toward corresponding templates but also to Direct detection protein achieved quantitatively. The QCM chip response obtained using epitope-mediated imprinting demonstrating comparable frequency shift chips immobilized with monoclonal...
ABSTRACT Here we report that severe combined immunodeficient (SCID) mice engrafted with human K562 cells (K562-SCID mice) can be used as an animal model to study dengue virus (DEN) infection. After intratumor injection into cell masses of PL046, a Taiwanese DEN-2 isolate, the K562-SCID showed neurological signs paralysis and died at approximately 2 weeks postinfection. In addition being detected in tumor masses, high titers were peripheral blood brain tissues, indicating DEN had replicated...
ABSTRACT We have found that NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) can be used to differentiate primary and secondary dengue virus infections. This is due the fact NS1-specific IgG antibody cannot detected before day 9 of illness for infection, so antibodies measured in acute-phase sera must come from previous infection. Comparison ELISA with envelope- membrane-specific capture IgM differentiation infections showed good correlation (95.90%...
ABSTRACT Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is zoonotic pathogen that prevalent in some Southeast Asian countries and causes acute humans. To evaluate the potential application of gene immunization to JEV infection, we characterized immune responses from mice intramuscularly injected with plasmid DNA encoding glycoproteins, including precursor membrane (prM) plus envelope (E) proteins nonstructural protein NS1. When expressing prM E, 70% immunized survived after...
Formalin-inactivated Japanese encephalitis virus (JEV) vaccines are widely available, but the effects of formalin inactivation on antigenic structure JEV and profile antibodies elicited after vaccination not well understood. We used a panel monoclonal (MAbs) to map live virus, untreated control (UCV), formalin-inactivated commercial vaccine (FICV), (FIV). The binding activity T16 MAb against Nakayama-derived FICV several strains FIV was significantly lower compared UCV. MAb, weakly...
The six core competencies of ACGME - patient care (PC), medical knowledge (MK), systems-based practice (SBP), practice-based learning and improvement (PBLI), professionalism (PROF), interpersonal communication skills (ICS) represent domains in which physicians must ultimately demonstrate competence. Although the ACGME's have been applied Taiwan with milestone project, application competences Family Medicine milestones for residency training not yet established. We recruited 61 family...
ABSTRACT Infection with Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, may cause acute in humans and induce severe cytopathic effects various types of cultured cells. We observed that JEV replication rendered infected baby hamster kidney (BHK-21) cells sensitive to the translational inhibitor hygromycin B or α-sarcine, which mock-infected were insensitive. However, little is known about whether any nonstructural (NS) proteins contribute virus-induced changes membrane...
To understand the antibody responses to dengue (DEN) nonstructural 1 (NS1) glycoprotein and their roles in protective immunity or pathogenesis of fever (DF) hemorrhagic (DHF), we have analyzed NS1-speccific IgM, IgA IgG antibodies from patients with DF DHF. An isotype-specific, indirect enzyme-linked immunosorbent assay (ELISA) was established by coating a NS1-specific monoclonal (MAb), D2/8-1, capture soluble NS1 antigens secreted culture supernatants Vero cells infected DEN virus. We...
ABSTRACT An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differentiate primary and secondary dengue virus infections serotypes of infection. For this report, we carried out retrospective seroepidemiologic studies on serum samples collected from residents Liuchiu Hsiang, Pingtung County, an isolated island in southern Taiwan during 1997-1998. The results demonstrated that good correlation existed between immunoglobulin G (IgG) ELISA plaque...
ABSTRACT Upon infection of Japanese encephalitis virus (JEV), baby hamster kidney (BHK-21) and Chinese ovary (CHO) cells were killed by a mechanism involved in apoptosis. While readily established variety cell lines, JEV persistence has never been successfully instituted BHK-21 CHO cells. Since stable expression human bcl-2 shown to delay JEV-induced apoptosis, this study we investigated whether could be such When constitutively expressing , but not its closest homolog, bcl-X L following...
ABSTRACT Envelope and membrane (E/M) nonstructural protein NS1 serotype-specific capture Immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) were developed to differentiate four dengue virus serotypes. A total of 93 anti-dengue IgM-positive serum samples collected between days 5 45 illness from 59 confirmed patients analyzed. The results showed that positive serotype specificity could be identified for 86.1 47.6% tested E/M-specific IgM antibodies versus 83.3 42.9%...
ABSTRACT Flaviviruses comprise a positive-sense RNA genome that replicates exclusively in the cytoplasm of infected cells. Whether flaviviruses require an activated nuclear factor(s) to complete their life cycle and trigger apoptosis cells remains elusive. Flavivirus infections quickly activate factor kappa B (NF-κB), salicylates have been shown inhibit NF-κB activation. In this study, we investigated whether suppress flavivirus replication virus-induced cultured dose-dependent inhibition,...
ABSTRACT The cross-reactive antibodies induced by flavivirus infections confound serodiagnosis and pathogenesis, especially in secondary caused antigenically closely related yet distinct flaviviruses. envelope (E) glycoprotein fusion peptide contains immunodominant determinants. Using a recombinant Japanese encephalitis virus (JEV) premembrane E expression plasmid producing JEV virus-like particles (VLPs), dramatic reductions cross-reactivity were produced the G106K-L107D (KD) double-mutant...
ABSTRACT Dengue virus (DV) was detected early in infected mosquito C6/36 cells by using indirect immunofluorescence (IF) conjunction with flow cytometry. Three fixation-permeabilization methods and three DV serotype 1 (DEN-1)-specific monoclonal antibodies, 8-8 (anti-E), 16-4 (anti-NS1), 15F3-1 were evaluated for the detection of DEN-1 cells. We found that these antibodies capable detecting as 24 h postinoculation a conventional IF stain. Both earlier showed greater number DV-positive than...
Abstract Background Ganoderma lucidum -derived polysaccharide (PS-G) can rapidly and effectively promote the activation maturation of immature dendritic cells (DCs), suggesting that PS-G possesses capacity to regulate immune responses. This study aimed clarify immunologic effect on monocyte-derived (MD-DCs) from asthmatic children allergic house dust mites. The MD-DCs were stimulated for 24 h with related allergen, Der p 1, in presence or absence PS-G. Cell surface markers phagocytic...
Abstract: Epidemic viral conjunctivitis is a highly contagious disease that encountered year-round. The causative agents are mainly adenoviruses and enteroviruses. It occurs most commonly upon infection with subgroup D of types 8, 19, or 37. For common corneal involvement human adenovirus type 8 epidemic keratoconjunctivitis, full-layer epithelial detachment rarely seen. Herein, we report three cases keratoconjunctivitis during an outbreak which manifested as large within few days. lesions...