A5006663526- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Virology and Viral Diseases
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Curcumin's Biomedical Applications
- Virus-based gene therapy research
- Child Nutrition and Water Access
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Fecal contamination and water quality
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- HIV Research and Treatment
- RNA regulation and disease
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Perovskite Materials and Applications
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
National Chung Hsing University
2008-2024
Chang Gung University
2003-2017
National Taiwan University
2001-2017
National Defense Medical Center
1997-2017
Mennonite Christian Hospital
2017
Tri-Service General Hospital
2017
Tungs' Taichung MetroHarbor Hospital
2017
National Dong Hwa University
2017
United States Department of Health and Human Services
2008
Centers for Disease Control and Prevention
2008
The antiviral effects of nitric oxide (NO) on Japanese encephalitis virus (JEV), a member the family Flaviviridae, were investigated in this study. In vitro, inhibition replication JEV gamma interferon-activated RAW 264.7 murine macrophages was correlated to cellular NO production. When cocultured with infected neuroblastoma N18 cells, cells also efficiently hindered contiguous bystanders, and anti-JEV effect could be reversed by an synthase (NOS) inhibitor, N-monomethyl-L-arginine acetate....
Curcumin, a natural compound and ingredient in curry, has antiinflammatory, antioxidant, anticarcinogenic properties. Previously, we reported that curcumin abrogated influenza virus infectivity by inhibiting hemagglutination (HA) activity. This study demonstrates novel mechanism which inhibits the of enveloped viruses. In all analyzed viruses, including virus, inhibited plaque formation. contrast, nonenveloped enterovirus 71 remained unaffected treatment. We evaluated effects on membrane...
Bovine lactoferrin (bLF) presents in milk and has been shown to inhibit several viral infections. Effective drugs are unavailable for the treatment of dengue virus (DENV) infection. In this study, we evaluated antiviral effect bLF against DENV infection vivo vitro. LF significantly inhibited four serotypes Vero cells. time-of-drug addition test, DENV-2 was remarkably when added during or prior occurrence attachment. We also revealed that bovine blocks binding between cellular membrane by...
Abstract Genotype I of Japanese encephalitis virus first appeared in Taiwan 2008. Phylogenetic analysis 37 viruses from pig farms 2009–2010 classified these into 2 unique subclusters genotype and suggested multiple introductions swift replacement III by Taiwan.
Background Genotype I (GI) Japanese encephalitis virus (JEV) that replaced GIII has become the dominant circulating in Asia. Currently, all registered live and inactivated JEV vaccines are derived from genotype III viruses. In Taiwan, compulsory vaccination policy recommends children receives four doses of formalin-inactivated Nakayama (GIII) vaccine. Methodology/Principal Findings To evaluate influence replacement on post-vaccination viral neutralizing ability by GI viruses, small panel...
Expansion of genotype I (GI) Japanese encephalitis viruses (JEV) has resulted in the replacement dominant III (GIII) viruses, raising serious public health concerns for using GIII virus-derived vaccines to effectively control JEV epidemics. Therefore, this study used swine as model estimate effectiveness live-attenuated vaccine against GI virus infection by comparing incidence stillbirth/abortion gilts from vaccinated and non-vaccinated pig farms during GI-circulation period. In total, 389...
Abstract Flavivirus virus-like particles (VLPs) exhibit a striking structural resemblance to viral particles, making them highly adaptable for various applications, including vaccines and diagnostics. Consequently, increasing VLPs production is important can be achieved by optimizing expression plasmids cell culture conditions. While attempting express genotype III (GIII) Japanese encephalitis virus (JEV) containing the G104H mutation in envelope (E) protein, we failed generate COS-1 cells....
Abstract Japanese encephalitis (JE) virus infects a number of host cells, either mosquitoes or vertebrates, in nature. The viral envelope (E) protein is known to interact with molecule(s) on the cell membrane during early stage infection. In this study, two sets variants including T1P1‐L4/T1P1‐S1 and CJN‐L1/CJN‐S1 derived from strains (T1P1 CJN) JE were used evaluate effects genomic variations entry. Each set variant (T1P1‐L4/T1P1‐S1 CJN‐L1/CJN‐S1) possessed single amino acid variation E...
ABSTRACT The cross-reactive antibodies induced by flavivirus infections confound serodiagnosis and pathogenesis, especially in secondary caused antigenically closely related yet distinct flaviviruses. envelope (E) glycoprotein fusion peptide contains immunodominant determinants. Using a recombinant Japanese encephalitis virus (JEV) premembrane E expression plasmid producing JEV virus-like particles (VLPs), dramatic reductions cross-reactivity were produced the G106K-L107D (KD) double-mutant...
Genotype I (GI) virus has replaced genotype III (GIII) as the dominant Japanese encephalitis (JEV) in epidemic area of Asia. The mechanism underlying replacement remains unclear. Therefore, we focused our current study on investigating roles mosquito vector and amplifying host(s) JEV by comparing replication ability GI GIII viruses. viruses had similar infection rates replicated to viral titers after blood meal feedings Culex tritaeniorhynchus. However, yielded a higher titer host-derived...
Abstract Genetically different subpopulations were identified and purified from Japanese Encephalitis virus (JEV). Those with small plaques (SPs; <2 mm in diameter), derived strains of T1P1, CJN, CC27, more competent than those large (LPs; >5 diameter) when passaged Neuro‐2a cells. Differences amino acids between SPs LPs each strain shown the viral envelope (E) protein. The acid at E‐306 was Glu LP but substituted by Lys SP T1P1 strain. A similar substitution occurred E‐138 CJN...
ABSTRACT Mouse mammary tumor virus (MMTV) has been speculated to be involved in human breast cancer. Companion animals, dogs, and cats with intimate contacts may contribute the transmission of MMTV between mouse human. The aim this study was detect MMTV-like nucleotide sequences canine feline tumors by nested PCR. Results showed that presence env LTR malignant 3.49% (3/86) 18.60% (16/86), respectively. For tumors, both found 22.22% (2/9). Nevertheless, also were detected normal glands dogs...
Virologic surveillance of Japanese encephalitis virus (JEV) relies on collecting pig blood specimens and adult mosquitoes in the past. Viral RNAs extracted from suffer low detecting positivity by reverse transcription PCR (RT-PCR). The oronasal transmission has been demonstrated experimentally infected pigs. This observation suggested could be useful source surveillance. However, role this unusual route remains unproven operational farm. In study, we explore feasibility using secretions...
Abstract Background Canine distemper virus (CDV) is present worldwide and produces a lethal systemic infection of wild domestic Canidae . Pre-existing antibodies acquired from vaccination or previous CDV might interfere the interpretation serologic diagnosis method. In addition, due to high similarity nucleic acid sequences between wild-type new vaccine strain, current PCR derived methods cannot be applied for definite confirmation CD infection. Hence, it worthy developing simple rapid...
The virulence of genotype I (GI) Japanese encephalitis virus (JEV) is under debate. We investigated differences in the GI and GIII JEV by calculating asymptomatic ratios based on serologic studies during GI- GIII-JEV endemic periods. results suggested equal among humans.
ABSTRACT Dengue vaccine candidates have been shown to improve safety and efficacy by altering the residues or accessibility of fusion loop on virus envelope protein domain II (DII FL ) in an ex vivo animal study. The current study aimed comprehensively investigate impact DII mutations antigenicity, immunogenicity, protective Japanese encephalitis (JEV) virus-like particles (VLPs) mice. We found G106K/L107D (KD) W101G/G106K/L107D (GKD) altered binding activity JEV VLP cross-reactive...