This study evaluates the post-hatching development of in vitro-produced (IVP) embryos until Day 14. On 7, IVP were either transferred to recipient uteruses or placed a (PHD) system. As control group, vivo-produced (IVV), Day-7 also uteruses. All groups collected on 14 and morphologically evaluated. Day-14 IVV used for quantification eight genes (PLAC8, CD9, SLC2A1, SLC2A3, KRT8, SOD2, HSP1A1, IFNT2) by reverse transcriptase qPCR. from PHD system smaller (2.92 ± 0.45 mm) had lower embryonic...

The objective of this study was to evaluate the effects different maturation systems on oocyte resistance after vitrification and phospholipid profile plasma membrane (PM). Four were tested: 1) in vitro using immature oocytes aspirated from slaughterhouse ovaries (CONT; n = 136); 2) obtained by ovum pick-up (OPU) unstimulated heifers (IMA; 433); 3) OPU stimulated (FSH; 444); 4) vivo 24 hours prior an injection GnRH (MII; 658). A sample matured each fresh group analyzed matrix associated...

The less differentiated the donor cells are used in nuclear transfer (NT), more easily they reprogrammed by recipient cytoplasm. In this context, mesenchymal stem (MSCs) appear as an alternative to nuclei for NT. amniotic fluid and adipose tissue sources of MSCs that have not been tested production cloned embryos cattle. objective study was isolate, characterize, use derived from (MSC-AF) (MSC-AT) produce calves. Isolation MSC-AF performed using vivo ultrasound-guided transvaginal...

Embryo production by intrafollicular oocyte transfer (IFOT) represents an alternative for of a large number embryos without requiring any hormones and only basic laboratory handling. We aimed to (1) evaluate the efficiency IFOT using immature oocytes (IFIOT) (2) compare embryo development after IFIOT fresh or vitrified oocytes. First, six IFIOTs were performed obtained ovum pickup. After insemination uterine flush recovery, 21.3% total transferred structures recovered excluding recipient's...

ABSTRACT: Wharton's jelly is a source of mesenchymal stem cells (MSCs) that had not yet been tested for bovine embryo production by nuclear transfer (NT). Thus, the objective this study was to isolate, characterize and test MSCs derived from pregnancy NT in cattle. The umbilical cord collected during calving (WJCs) were isolated explant cultured Dulbecco's Modified Eagle Medium. Skin Fibroblasts (FB) after 6 months life. Morphological analysis performed bright field scanning electron...

Abstract In vitro produced embryos are still sensitive to the freezing process which can be explained, in part, by high‐lipid accumulation that characterizes these embryos. Therefore, we aimed evaluate effect of delipidating agents, L‐carnitine and trans‐10 cis‐12 conjugated linoleic acid (CLA) isomer, on blastocyst development, lipid content, gene expression cryotolerance when added embryo culture media. Embryos were cultured four different media: T1: control ( n = 616), synthetic oviduct...

This study aimed to evaluate the effect of meiotic arrest using phosphodiesterase type 3A (PDE 3A) inhibitors, cilostamide and C-type natriuretic peptide (NPPC), on pre-maturation (PM) oocytes be used in production cloned embryos. Nuclear maturation, vitro embryo (IVP), somatic cell nuclear transfer (SCNT) parthenogenetic activation (PA), total cells number embryos were evaluated. The results analysed by chi-squared Kruskal-Wallis test with a P-value 0.05) between control PM, both for...

Transcervical artificial insemination (AI) after the surgical incision of cervical folds (SICF) could represent a valid alternative to laparoscopic AI when frozen thawed semen is used. The aim this experiment was compare pregnancy (PR) and lambing rates (LR) ewes submitted either transcervical SICF or using semen. Pregnant at term (n = 80) were allocated in two experimental groups. After lambing, one group 39) SICF. remaining that regularly lambed 40). Six months later, oestrous cycle both...

In the present study, four experimental groups were used: fresh embryos, cultured during in vitro maturation and culture media supplemented with bovine serum albumin (BSA) (fresh BSA) or fetal (FBS) FBS); two of cryopreserved thawed produced under same conditions (frozen BSA frozen FBS). Experiment 1 evaluated protein source effect on embryo development response to cryopreservation. At day 7, half expanded blastocysts (Bx) from each group warmed other used as controls. After warming, embryos...

RESUMO Os objetivos do presente estudo foram analisar a ultraestrutura espermatozoide jundiá amazônico e avaliar sua criopreservação com três agentes crioprotetores (metanol 10%, DMSO 10% etilenoglicol 10%) duas soluções ativadoras (NaCl 0,29% NaHCO3 1%). Como diluente, foi utilizada uma solução de glicose 5%, sendo o sêmen envasado em palhetas 0,25mL congelado vapor nitrogênio (botijão dry shipper). No fresco, apresentou comprimento 25,46±2,54μm, cabeça esférica (1,51±0,18μm), ausência...

There is no consensus about the occurrence of transuterine embryo migration under natural breeding circumstances, neither data related to this phenomenon for zebu cattle. In study, 5431 reproductive tracts Nellore cows and heifers were evaluated in an abattoir state Mato Grosso do Sul. A total 1030 animals (19.0%) pregnant at time slaughter (including 2 twin pregnancies). Regarding singleton pregnancies (n = 1028), 39.5% them, fetus was located left uterine horn remaining 60.5% right. These...

This study aimed to evaluate the effect of exogenous recombinant bovine somatotropin (rbST) on plasma concentrations insulin-like growth factor I (IGF-I), insulin and semen quality bulls. Twenty bulls (Aberdeen Angus Brangus) were divided by breed into two groups. Placebo group was injected with NaCl 0.9% (s.c.) treatment rbST (s.c., 500 mg) at days 0 14 experiment. Immediately after collection, blood samples taken 0, 14, 28, 42 56 Semen also collected day 70 Evaluation sperm motility...

ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF identify the main phospholipids (PL). After that, in vivo (D6) vitrified (n=52), non-vitrified used as control (n=42). warming, vitro cultured assess re-expansion and hatching at 24 48 hours. Finally, hours, hatched RT-qPCR searching for BCL2A1, BAK, BAX CASP3 genes. For MALDI-TOF, ion intensity was expressed arbitrary units. Blastocyst development compared by Qui-square (P< 0.05). Among most representative PL...

Abstract This study aimed to compare the effects a commercial milk-based extender and self-made egg yolk had on quality of canine semen stored at two different temperatures, 5ºC or 15ºC. The ejaculate obtained was split into aliquots equal volume diluted with milk extender. final concentration 100×106 spermatozoa/mL. Diluted placed in transport containers maintained storage temperatures chilled assessed 12, 24, 36 hours after storage. Semen higher motility, vigour, plasma membrane integrity...

ABSTRACT: Determining if reproductive failures in ewes at the semiarid region state of Bahia are related to consumption species Cenostigma pyramidale (Tul.) Gagnon & G.P. Lewis, and this study was developed using pregnant divided into six groups: G1, G2, G3, G4 with animals each, G5 G6 ten animals. Each group received fence leaves proportion 1%, 2%, 0.5%, 0.25% live weight (LW) respectively; G6, receiving 0.5% LW, respectively, Control Group, comprising 16 ewes, were grass feeding...

In vitro embryos production from prepubertal heifers can help contribute to breeding programs; however, strategies are necessary increase their embryo production. The aim of this study was investigate the effects two nutritional plans on oocyte recovery, and growth performance Nelore heifers. Thirty-four with age 6.5 months were divided into feeding treatments (NP1 NP2). NP1 diets served as control NP2 formulated contain an average 1.22-fold more energy than NP1. After 3 supplementation,...

For the development of in vitro produced (IVP) as well vivo bovine embryos, it is extremely important that their energy metabolism works properly because embryo must be able to metabolize substrates are necessary for producing energy. Lipids play an role early embryonic development, acting source oocytes and embryos. However, known mainly IVP, accumulate large amounts lipids cytoplasm. Although they have been associated with reduced survival embryos following cryopreservation. There evidence...

The study evaluated qualitative and quantitative characteristics of Amazonian jundiá semen.The experiment was carried out in February 2015 at Cia do Peixe, a company located Goiás state, Brazil, that specialises fish fry production.Three male L. marmoratus farmed dugout ponds, weighing 1.34 ± 0.29 kg measuring 39.33 1.15 cm length, were tested.The specimens captured transferred to 1000-L tank the Reproduction Laboratory abovementioned company.Water temperature measured 7:00 a.m. 5:00 p.m.,...