A5071443735- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Genetic and phenotypic traits in livestock
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Reproductive Physiology in Livestock
- Genetic Syndromes and Imprinting
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Bioenergy crop production and management
- Meat and Animal Product Quality
- Genetic Mapping and Diversity in Plants and Animals
- Cancer-related molecular mechanisms research
- Reproductive Health and Technologies
- Birth, Development, and Health
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Seed Germination and Physiology
- Insect Pest Control Strategies
- Growth Hormone and Insulin-like Growth Factors
- Mesenchymal stem cell research
Universidade Federal de Uberlândia
2016-2025
Brazilian Agricultural Research Corporation
2016-2025
King's College London
2021
Universidade de Brasília
2008-2017
National Center for Genetic Engineering and Biotechnology
2017
Institute for Animal Reproduction
2013
University of Miami
2009
Brazilian Santa Inês (SI) sheep are very well-adapted to the tropical conditions of Brazil and an important source animal protein. A high rate twin births was reported in some SI flocks. Growth Differentiation Factor 9 (GDF9) Bone Morphogenetic Protein 15 (BMP15) first two genes expressed by oocyte be associated with increased ovulation sheep. All GDF9 BMP15 variants characterized, until now, present same phenotype: heterozygote ewes have mutated homozygotes sterile. In this study, we found...
To identify the genes related to oocyte competence, we quantified transcripts for candidate in oocytes (H1Foo, H2A, H3A, GHR, GDF9, BMP15, OOSP1) and cumulus cells (FSHR, EGFR, PTX3, IGFII) using follicle size model select of better developmental quality. Follicles were dissected distributed into four groups according diameter as follows: 1.0-3.0, 3.1-6.0, 6.1-8.0 >or=8.1 mm. Cumulus-oocyte complexes (COCs) released, classified morphologically, matured, fertilised cultured vitro or denuded...
To improve the quality of in vitro produced (IVP) embryos and reduce pregnancy losses, we proposed to components synthetic oviduct fluid (SOF) medium by 0% (SOF100), 50% (SOF50), 75% (SOF25). First, under these three treatments were evaluated for production, quality, lipid content, gene expression, methylation patterns. The results indicated that all parameters analyzed similar across (P > 0.05), suggesting reducing media does not affect embryo development quality. Subsequently, selected...
Abstract Stem cells are undifferentiated that exhibit a bivalent chromatin state determines their fate. These have potential applications in human and animal health livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome the main obstacle correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes somatic promising strategy contribute more...
Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic of good quality. Due the impossibility collecting blood slaughtered animals, extraction solid tissues necessary. The objective this study was describe a protocol swine skin, adipose, brain, liver, kidney and muscle We obtained high molecular weight quality, shown agarose gel amplification two fragments, 605bp 891pb, PCR. Spectrophotometric analysis concentration showed...
Embryos produced by hormonal superstimulation have been used as an in vivo control most published research on embryo gene expression.However, it is not known if this the appropriate for expression profile studies.We compared of GRB-10, IGF-II, IGF-IIR, MnSOD, GPX-4, catalase, BAX, and interferon-τ genes, embryos superovulation (SOV), vitro fertilization (IVF) or without any stimulus (NOV).GRB-10 was less expressed NOV than IVF embryos, whereas no differences were found other genes.The genes...
This study evaluates the post-hatching development of in vitro-produced (IVP) embryos until Day 14. On 7, IVP were either transferred to recipient uteruses or placed a (PHD) system. As control group, vivo-produced (IVV), Day-7 also uteruses. All groups collected on 14 and morphologically evaluated. Day-14 IVV used for quantification eight genes (PLAC8, CD9, SLC2A1, SLC2A3, KRT8, SOD2, HSP1A1, IFNT2) by reverse transcriptase qPCR. from PHD system smaller (2.92 ± 0.45 mm) had lower embryonic...
Oocyte quality is one of the most important aspects in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern imprinted genes be established on differentially methylated regions (DMR). The insulin-like factor 2 (IGF2) gene an factor, it several mammalian species. aim this study was to evaluate DMR last exon IGF2 immature mature bovine oocytes with different developmental competencies. Mature from large...
Contents The aim of this study was to investigate the expression profile candidate genes involved in competence during oocyte growth. ( BMP 15, OOSP 1, H1FOO , H 2A, 3A, 4, SLBP DNMT 3B, HAT HDAC 2 and SUV 39H1 ) were selected because their possible involvement determining developmental competence. Pre‐antral antral follicles isolated from ovaries Z ebu B os indicus cows, measured classified into following categories according diameter: (i) oocytes primordial follicles: diameter <20 μm,...
Sublethal stress treatment has been reported to enhance gametes’ performance in subsequent procedures, such as cryopreservation. The aim of the present study was evaluate effect different equilibration times between termination a sublethal hydrostatic pressure (HP) and initiation vitrification on post-thaw survival, continued vitro development, hatching rate gene expression selected candidate genes vitro-produced (IVP) expanded bovine blastocysts. Day 7 IVP blastocysts were subjected 600 bar...
Embryo production by intrafollicular oocyte transfer (IFOT) represents an alternative for of a large number embryos without requiring any hormones and only basic laboratory handling. We aimed to (1) evaluate the efficiency IFOT using immature oocytes (IFIOT) (2) compare embryo development after IFIOT fresh or vitrified oocytes. First, six IFIOTs were performed obtained ovum pickup. After insemination uterine flush recovery, 21.3% total transferred structures recovered excluding recipient's...
Abstract The objectives of this study were to investigate the effect sexing by flow cytometry on methylation patterns IGF2 and IGF2R genes. Frozen‐thawed, unsorted, sex‐sorted sperm samples from four Nellore bulls used. Each ejaculate was separated into three fractions: non‐sexed (NS), sexed for X‐sperm (SX), Y‐sperm (SY). Sperm isolated extender, cryoprotectant, other cell types centrifugation a 40:70% Percoll gradient, pellets used genomic DNA isolation. analyses bisulfite sequencing....
Dopa decarboxylase (DDC) synthesizes serotonin in the developing mouse heart where it is encoded by Ddc_exon1a, a tissue-specific paternally expressed imprinted gene. Ddc_exon1a shares an imprinting control region (ICR) with imprinted, maternally (outside of central nervous system) Grb10 gene on chromosome 11, but little else known about expression Ddc_exon1a. Fluorescent immunostaining localizes DDC to myocardium pre-natal heart, susceptible abnormal development and implicated congenital...