A5080405012- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Effects of Environmental Stressors on Livestock
- Rural Development and Agriculture
- Ovarian function and disorders
- Sperm and Testicular Function
- Genetic Mapping and Diversity in Plants and Animals
- Animal Behavior and Welfare Studies
- Milk Quality and Mastitis in Dairy Cows
- Helminth infection and control
- Renal and related cancers
- Kruppel-like factors research
- TGF-β signaling in diseases
- Birth, Development, and Health
- Molecular Biology Techniques and Applications
- Diphtheria, Corynebacterium, and Tetanus
- Tissue Engineering and Regenerative Medicine
- Animal health and immunology
- Clostridium difficile and Clostridium perfringens research
- Hedgehog Signaling Pathway Studies
- Trace Elements in Health
- Reproductive biology and impacts on aquatic species
- Prion Diseases and Protein Misfolding
Brazilian Agricultural Research Corporation
2008-2021
South Dakota State University
2013
Beltsville Agricultural Research Center
2013
Agricultural Research Service
2013
MRC Centre for Reproductive Health
2002-2012
The Queen's Medical Research Institute
2011
Medical Research Council
2011
University of Edinburgh
1996-2011
Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
1994
Brazilian Santa Inês (SI) sheep are very well-adapted to the tropical conditions of Brazil and an important source animal protein. A high rate twin births was reported in some SI flocks. Growth Differentiation Factor 9 (GDF9) Bone Morphogenetic Protein 15 (BMP15) first two genes expressed by oocyte be associated with increased ovulation sheep. All GDF9 BMP15 variants characterized, until now, present same phenotype: heterozygote ewes have mutated homozygotes sterile. In this study, we found...
In this study, we investigated follicular dynamics and ovarian steroid secretion during the early luteal phases of estrous cycle in sheep. Six Finn-Merino ewes with autotransplanted ovaries were monitored for 10 days phase subsequent after regression was induced cloprostenol (a potent analogue prostaglandin F2α). Over period, diameter measured by serial ultrasound scans, concentration gonadotropins steroids venous blood at intervals 6-12 h. All animals had an LH surge (Day 0) 59 ± 4.7 h...
This review offers an overview of the basic characteristics in vivo embryo technologies, their current status, main findings and advances gained recent years, outstanding subjects for increasing efficiency. The use superovulation transfer procedures remains affected by a high variability ovulatory response to hormonal treatment low variable number transferable embryos offspring obtained. has been classically identified with both extrinsic (source, purity gonadotrophins protocol...
One of the hallmarks polycystic ovary syndrome (PCOS) is increased ovarian androgen secretion that contributes to ovarian, hormonal, and metabolic features this condition. Thecal cells from women with PCOS have an enhanced capacity for synthesis. To investigate whether propensity a potential cause, rather than consequence, PCOS, we used ovine prenatal androgenization model assessed ewes at 11 months age. Pregnant Scottish Greyface were administered 100 mg testosterone propionate (TP) or...
The FecB (Booroola) mutation, which leads to increased ovulation rates and multiple births in sheep, is now known occur the signaling domain of bone morphogenic protein (BMP)-1B receptor. We examined effect mutation on responsiveness granulosa (GC) theca cells (TC) BMPs other local regulators using tissue from animals with (FecB/B) without (Fec+/+) mutation. Experiments BMP-2, -4, -6 (0.005–50 ng/ml), their interaction IGF-I (0.1–10 ng/ml LR3 analog) gonadotropins, proliferation...
Summary Litter size ( LS ) in sheep is determined mainly by ovulation rate OR ). Several polymorphisms have been identified the growth differentiation factor 9 GDF gene that result an increase and prolificacy of sheep. Screening databank Brazilian Sheep Breeders Association for triplet delivery, we flocks prolific Ile de France ewes. After resequencing 9, a point mutation (c.943C>T) was identified, resulting non‐conservative amino acid change (p.Arg315Cys) cleavage site propeptide. This...
The dynamics of ovarian follicular development and its relationship to pituitary hormones during seasonal anoestrus were investigated for 10 days in nine ewes with autotransplanted ovaries a longitudinal study. size position the ovary individual follicles over 2.5 mm diameter recorded by daily ultrasonography. Samples jugular venous blood collected at intervals 12 h, before after GnRH challenge (250 ng GnRH, i.v.) so that basal LH-stimulated steroid secretion could be determined. Throughout...
The identification of a single gene (Booroola FecB) associated with significant increase in the ovulation rate sheep provides powerful tool for study factors regulating selection preovulatory follicles. ovarian secretion dimeric inhibin A was investigated and related to steroids, concentration gonadotropins, pattern follicular development during early luteal phases ewes an autotransplant or without FecB gene. presence large estrogenic follicles, being high phase falling after LH surge (P <...
Little is known about the role of activin B during folliculogenesis. This study investigated expression levels activin/inhibin subunits (βA, βB, and α), steroid enzyme, gonadotrophin receptors in theca (TC) granulosa cells (GC) by QPCR A inhibin protein follicular fluid (FF) developing sheep follicles estrus anestrus. The effect on androgen production from primary TC cultures vitro was also assessed. During folliculogenesis, anestrus estrus, FF concentrations thecal GC βB mRNA decreased as...
The relationship between changes in body condition score (BCS) during the postpartum and fertility beef cows suckling calves under extensive conditions were investigated. Cows subjected to four BCS evaluations over period, starting around one month after calving. In second evaluation treated with medroxy-progesterone acetate impregnated pessaries received an injection of estradiol benzoate. At third evaluation, removed separated from for 96 hours, which time estrous was observed twice a day,...
INTRODUCTION: The Santa Ines (SI) sheep is very adapted to the dry weather of Brazilian northeast region and an important source animal protein for local populations. It not rare a high rate twining within Inês Twinning desirable in order increase profitability, this trait can be disseminated trough assisted selection. Growth Differentiation Factor 9 (GDF9) Bone Morphogenetic Protein 15 (BMP15) are first two growth factors synthesized oocyte, associated with increased ovulation sheep. Five...
The identification of a single gene (Booroola FecB) associated with significant increase in the ovulation rate sheep provides powerful tool for study factors regulating selection preovulatory follicles.