A5100639859- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Mycobacterium research and diagnosis
- Antifungal resistance and susceptibility
- Parathyroid Disorders and Treatments
- Biosensors and Analytical Detection
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Cancer Research and Treatments
- Gut microbiota and health
- Protist diversity and phylogeny
- Pulmonary Hypertension Research and Treatments
- Cytokine Signaling Pathways and Interactions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Epigenetics and DNA Methylation
- Insect symbiosis and bacterial influences
- Bacteriophages and microbial interactions
- Immune responses and vaccinations
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Ear Surgery and Otitis Media
- Parasitic infections in humans and animals
- Rabies epidemiology and control
- Nephrotoxicity and Medicinal Plants
Jilin University
2014-2025
WuXi AppTec (China)
2025
Huazhong University of Science and Technology
2004-2024
Beth Israel Deaconess Hospital
2015-2024
Beijing Children’s Hospital
2024
Capital Medical University
2019-2024
Hubei University of Medicine
2022-2023
Shaanxi University of Science and Technology
2023
Beth Israel Deaconess Medical Center
2016-2022
Shandong Academy of Sciences
2022
The formation of extracellular traps (ETs) has recently been recognized as a novel defense mechanism in several types innate immune cells. It suggested that these structures are toxic to microbes and contribute significantly killing pathogens. However, the role ETs formed by macrophages (METs) against remains little known. In this study, we demonstrated subset murine J774A.1 macrophage cell line (8% 17%) peritoneal (8.5% 15%) form METs-like (METs-LS) response Escherichia coli Candida...
An ability to promote therapeutic immune cells recognize cancer is important for the success of cell-based immunotherapy. We present a synthetic method functionalizing surface natural killer (NK) with supramolecular aptamer-based polyvalent antibody mimic (PAM). The PAM synthesized on cell through nucleic acid assembly and hybridization. data show that has superiority over its monovalent counterpart in powering NKs bind cells, PAM-engineered NK exhibit capability killing more effectively....
Abstract Background DNA methylation plays an essential role in regulating gene expression under a variety of conditions and it has therefore been hypothesized to underlie the transitions between life cycle stages parasitic nematodes. So far, however, 5'-cytosine not detected during any developmental stage nematode Caenorhabditis elegans . Given new availability high-resolution detection methods, investigation can now be carried out. Results Here, using MethylC-seq, we present first study...
Thymol (THY) was found to have in vitro antifungal activity against 24 fluconazole (FLC)-resistant and 12 FLC-susceptible clinical isolates of Candida albicans , standard strain ATCC 10231 one experimentally induced FLC-resistant C. S-1. In addition, synergism observed for with combinations THY–FLC THY–amphotericin B (AMB) evaluated by the chequerboard microdilution method. The interaction intensity determined spectrophotometry assay, nature interactions assessed using two non-parametric...
The intestinal phase is critical for trichinellosis caused by Trichinella spiralis (T. spiralis), as it determines both process and consequences of the disease. Several previous studies have reported that T. induces initial predominance a Th1 response during intestine stage subsequent Th2 muscle stage. In present study, immune cells cytokine profile were investigated in mice infected with spiralis. results showed number eosinophils, goblet cells, mucosal mast 33D1+ dendritic (DCs) increased...
Abstract Trichinellosis, which is caused by Trichinella spiralis ( T. ), a serious zoonosis. Pigs play an important role in the transmission of human trichinellosis. Characterizing immune response to infection key elucidating host–parasite interactions. However, most studies on have employed murine models. In this study, we investigated pigs. The results showed that average numbers larvae per gram (lpg) for 100-muscle (ML), 1000-ML, and 10 000-ML groups were 1.502, 35.947, 398.811,...
Abstract Trichinella spiralis ( T. ) has been reported to induce inflammation, which can cause immune system dysregulation. Myeloid differentiation primary response gene 88 (MyD88) is implicated in inflammation signalling pathways. TJ-M2010-5 a novel MyD88 inhibitor with remarkable protective effects against several diseases. However, the precise mechanism of TJ-M2010-5’s involvement spleen impairment and early infection yet be fully elucidated. This study analysed histological, macrophage...
Abstract Paclitaxel, one of the most commonly used anticancer agents, is employed in treatment a range malignant tumors. However, resistance major barriers to successful therapy. Despite its clinical relevance, molecular mechanisms underlying paclitaxel remain poorly understood. In this protocol, we describe methods for establishing paclitaxel‐resistant tumor models both vitro and vivo, how investigated resistance. Additionally, evaluated potential combination therapies overcome these...
The active multidrug efflux pump (EP) has been described as one of the mechanisms involved in natural drug resistance bacteria, such mycobacteria. As a result, development pumps inhibitors (EPIs) is an important topic. In this study, checkerboard synergy assay indicated that farnesol both decreased minimum inhibitory concentration (MIC) ethidium bromide (EtBr) 8-fold against Mycobacterium smegmatis (M. smegmatis) mc2155 ATCC 700084 when incorporated at 32 μg/mL (FICI = 0.625) and MIC 4-fold...
Piperine, a major plant alkaloid found in black pepper (Piper nigrum) and long longum), has shown potential for inhibiting the efflux pump (EP) of Staphylococcus aureus. In this study, modulation assay showed that piperine could decrease MIC ethidium bromide (EtBr) twofold at 32 μg ml(-1) fourfold 64 μg against Mycobacterium smegmatis mc(2) 155 ATCC 700084. A real-time, 96-well plate fluorometric method was employed to evaluate EP inhibition ability M. smegmatis. Reserpine,...
Reports have shown that oleanolic acid (OA), a triterpenoid, exists widely in food, medicinal herbs and other plants, it has antimycobacterial activity against the Mycobacterium tuberculosis strain H37Rv (ATCC 27294). In this study was found OA had properties eight clinical isolates of M. MICs drug-sensitive drug-resistant were 50-100 100-200 microg ml(-1), respectively. The combination with isoniazid (INH), rifampicin (RMP) or ethambutol (EMB) showed favourable synergistic effects six...
MAVS is a critical adaptor required for activating an innate antiviral immune response against viral infection. The activation of requires modification the Lys63-linked ubiquitination and formation prion-like aggregates. However, molecular mechanisms regulating activity remain largely obscured. In this study, we identified deubiquitinase YOD1, also known as member ovarian tumor family, negative regulator in both human murine cells. YOD1 was recruited to mitochondria interact with through its...
A new type of Ag@SnS<sub>2</sub>was established with Ag decorated on SnS<sub>2</sub>surface for improved photothermal conversion capability. Ag@SnS<sub>2</sub>exhibited a robust<italic>in vitro</italic>antibacterial effect and<italic>in vivo</italic>wound disinfection under NIR laser irradiation.
A finite-element analysis for static behavior of middle ear under variation the middle-ear pressure was conducted in a 3D model human by combining hyperelastic Mooney-Rivlin material and geometry nonlinearity. An empirical formula then developed to calculate parameters soft tissues as stress-dependent elastic modulus relative pressure. Dynamic response sound canal predicted various positive negative pressures. The results from indicate that produces displacements tympanic membrane (TM)...
A checkerboard microdilution method, performed according to the recommendations of National Committee for Clinical Laboratory Standards, was used study in vitro interaction fluconazole and allicin 24 fluconazole-resistant clinical isolates Candida albicans, one experimentally induced strain S-1, ATCC type 10231. The intensity determined by spectrophotometric methods visual reading assay, nature interactions assessed using two nonparametric approaches [fractional inhibitory concentration...
Trichinellosis has major economic impacts on animal husbandry and food safety, the control elimination of trichinellosis is a objective veterinary medicine. A gene encoding serine protease Trichinella spiralis (Ts-Adsp) was identified by immunoscreening an adult T. cDNA library. In this study, recombinant Ts-Adsp protein (rTs-Adsp) cloned expressed in prokaryotic expression system purified Ni-affinity chromatography. To determine whether rTs-Adsp potential vaccine candidate for infection, we...
In this paper, a newly constructed three-dimensional finite element (FE) model of the human ear based on histological sections left temporal bone is reported. The otitis media with effu-sion was simulated in variable fluid levels middle ear. interfaces among air, structure, and canal cavity were identified acoustic-structure-fluid coupled FE analysis conducted when level varied from zero to full fill cavity. results show how displacements tympanic membrane stapes footplate or transfer...