A5033927550- Neuropeptides and Animal Physiology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Receptor Mechanisms and Signaling
- Diet, Metabolism, and Disease
- Heart Rate Variability and Autonomic Control
- Blood Coagulation and Thrombosis Mechanisms
- Neuroendocrine regulation and behavior
- Nitric Oxide and Endothelin Effects
- Neuroscience of respiration and sleep
- Peptidase Inhibition and Analysis
- Mast cells and histamine
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Phytochemical compounds biological activities
- Regulation of Appetite and Obesity
- Pharmacological Effects and Assays
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Pancreatic function and diabetes
- Neurotransmitter Receptor Influence on Behavior
- Sleep and Wakefulness Research
- Cardiovascular and exercise physiology
- Urticaria and Related Conditions
- Fatty Acid Research and Health
- Cardiovascular Function and Risk Factors
- Pharmacological Receptor Mechanisms and Effects
Université Laval
2006-2022
Centre hospitalier de l'Université Laval
1992-2020
Centre hospitalier universitaire de Québec
2006-2018
Wilfrid Laurier University
1994-2013
Hypertension Institute
2000
Hypertension Canada
1999
Queen's Medical Centre
1990-1992
Université du Québec à Montréal
1986-1987
Institut National de la Recherche Scientifique
1986-1987
Université de Sherbrooke
1984-1987
Extracellular nucleotides are vasoactive molecules. The concentrations of these molecules regulated by ectonucleotidases. In this study, we investigated the role blood vessel ectonucleotidase NTPDase1, in vasoconstrictor effect using Entpd1−/− mice. Immunofluorescence, enzyme histochemistry, and HPLC analysis were used to evaluate both NTPDase expression activity arteries isolated vascular smooth muscle cells (VSMCs). Vascular reactivity was evaluated vitro mean arterial pressure recorded...
This study was designed to examine the effects of a high-fat, high-sucrose (HFHS) diet on vascular and metabolic actions insulin. Male rats were randomized receive an HFHS or regular chow for 4 wk. In first series experiments, had pulsed Doppler flow probes intravascular catheters implanted measure blood pressure, heart rate, regional flows. Insulin sensitivity responses insulin assessed during euglycemic hyperinsulinemic clamp performed in conscious rats. second new groups used skeletal...
Previous studies have shown that renal autoregulation dynamically stabilizes blood flow (RBF). The role of nerves, particularly a baroreflex component, in dynamic regulation RBF remains unclear. relative roles and mesenteric nerves the superior artery (MBF) are similarly In this study, transfer function analysis was used to identify autoregulatory components MBF Wistar rats young spontaneously hypertensive (SHR) anesthetized with isoflurane or halothane. showed effective both RBF, as did...
Bradykinin (BK)-mediated angioedema (AE) states are rare acquired or hereditary conditions involving localized edema of the subcutaneous and submucosal tissues. Citrated plasma from healthy volunteers patients with (HAE) normal level C1-inhibitor (C1-INH) was used to investigate pathways BK formation breakdown relevant AE physiopathology. The half-life (100 nM) added 34 s, a value that increased ~12-fold when angiotensin converting enzyme (ACE) inhibitor enalaprilat (130 (enzyme immunoassay...
1. This study was undertaken to further investigate the effects of a sucrose-enriched diet on vascular function and insulin sensitivity in rats. 2. Male Sprague-Dawley rats were randomized receive sucrose- or regular rat chow-diet for 4 weeks. A first group chow-fed instrumented with pulsed Doppler flow probes intravascular catheters determine blood pressure, heart rate, regional flows conscious Insulin assessed by euglycemic hyperinsulinemic clamp technique. Glucose transport activity...
The insulin-mediated vasodilator effect has been proposed as an important physiological determinant of insulin action on glucose disposal in normotensive humans. present study was designed to further examine the acute regional hemodynamic effects different vascular beds and explore relationships between sensitivity during euglycemic hyperinsulinemic clamps conscious Wistar-Kyoto (WKY) rats spontaneously hypertensive (SHR). were instrumented with intravascular catheters pulsed Doppler flow...
We recently investigated the pathways of immunoreactive bradykinin (iBK) formation in fresh blood normal volunteers and patients with hereditary angioedema due to C1-esterase inhibitor deficiency (HAE-1/-2). Herein, we adapted techniques small volumes (200 μl) previously frozen citrated plasma further analyzed mechanisms iBK additional biotechnological inhibitors.Measurable was observed under stimulation tissue kallikrein (KLK-1, 10 nM), particulate material Kontact-APTT (concentration...
We reported evidence of bradykinin (BK) regeneration from C-terminal extended BK sequences that behave as peptidase-activated B2 receptor (B2R) agonists. Further to these in vitro studies, we carried out vivo experiments verify hemodynamic effects analogs exhibiting variable susceptibility toward vascular and blood plasma peptidases. Rats were anesthetized instrumented record pressure heart rate responses bolus intravenous (i.v.) injection increasing doses BK, B-9972...
The cardiovascular effects of bilateral injection into the hypothalamic paraventricular nuclei selective μ‐, δ‐, and κ‐opioid receptor agonists were investigated in conscious, unrestrained Wistar Kyoto rats, chronically instrumented with pulsed Doppler flow probes for measurement regional haemodynamics. μ‐agonist [D‐Ala 2 , MePhe 4 Gly 5 ol]enkephalin (DAMGO), injected bilaterally (0.01‐1.0 nmol), caused increases blood pressure, tachycardias, vasoconstriction renal superior mesenteric...
We investigated the effects of treatment with tempol (an antioxidant) on vascular and metabolic dysfunction induced by a high-fat high-sucrose (HFHS) diet. Rats were randomized to receive an HFHS or chow diet without (1.5 mmol·(kg body mass)(-1)·day(-1)) for 4 weeks. Blood pressure, heart rate, blood flow measured in rats using intravascular catheters Doppler probes. Insulin sensitivity responses insulin assessed during euglycemic-hyperinsulinemic clamp. In-vitro studies performed evaluate...
We investigated the long-term effect of metformin treatment on blood pressure, insulin sensitivity, and vascular responses to in conscious spontaneously hypertensive rats (SHR). The were instrumented with intravascular catheters pulsed Doppler flow probes measure heart rate, flow. Insulin sensitivity was assessed by euglycemic hyperinsulinemic clamp technique. Two groups SHR received (100 or 300 mg ⋅ kg − 1 day ) for 3 wk while another group a Wistar Kyoto (WKY) left untreated. found that...
This study was designed to investigate the effects of a sucrose diet on vascular and metabolic actions insulin in spontaneously hypertensive rats (SHR). Male SHR were randomized receive or regular chow for 4 wk. Age-matched, chow-fed Wistar-Kyoto (WKY) used as normotensive control. In first series experiments, three groups had pulsed Doppler flow probes intravascular catheters implanted determine blood pressure, heart rate, flows. Insulin sensitivity assessed during euglycemic...
The dynamic relationship among three major vascular control mechanisms that operate on large fractions of cardiac output: arterial baroreflex and renal mesenteric autoregulation, was investigated in conscious rats. Wistar spontaneously hypertensive rats were studied their home cages 10 days after implantation pulsed Doppler flow probes. There an oscillation blood pressure centered at 0.45 Hz is associated with operation baroreflexes. Hindquarters displayed a featureless, "1/f " power...