A5043214460- Renal Diseases and Glomerulopathies
- Ion Transport and Channel Regulation
- Dialysis and Renal Disease Management
- X-ray Diffraction in Crystallography
- Gastric Cancer Management and Outcomes
- Chronic Kidney Disease and Diabetes
- Crystallization and Solubility Studies
- Hormonal Regulation and Hypertension
- Ion channel regulation and function
- Electrolyte and hormonal disorders
- Microstructure and Mechanical Properties of Steels
- Magnesium in Health and Disease
- Central Venous Catheters and Hemodialysis
- Metallurgical Processes and Thermodynamics
- Semiconductor materials and devices
- Metastasis and carcinoma case studies
- Liver Disease Diagnosis and Treatment
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Organic Chemistry Cycloaddition Reactions
- Kidney Stones and Urolithiasis Treatments
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Fluorine in Organic Chemistry
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Diabetes Treatment and Management
Saitama Medical University
2015-2024
Social Insurance Saitama Chuo Hospital
2015-2024
Chuo University
2014
Tohoku University
2001-2009
Nagoya Institute of Technology
2006-2007
Nippon Steel (Japan)
2001-2006
National Hospital for Neurology and Neurosurgery
2004
National Center of Neurology and Psychiatry
2004
University College London
2004
Osaka University
2004
Two mercurial-inhibitable water-transporting proteins have been identified: CHIP28, an erythrocyte water channel also expressed in kidney tubules and selected extrarenal epithelia, WCH-CD, a collecting duct channel. In searching for protein responsible the high transcellular permeability lung alveolus, we cloned 32-kDa (mercurial-insensitive (MIWC)) from rat cDNA library with several novel features. Water was strongly increased Xenopus oocytes expressing MIWC mercurial-insensitive manner,...
A native oxidation of by anodization in mixed solutions glycol and water (AGW) is described. detailed comparison between the AGW process aqueous (AAS) shows basic importance (i) simultaneous oxide dissolution during growth (ii) initial electrochemical active‐passive transition anode. The results demonstrate substantial superiority over various AAS processes. Factors relevant for uniform, stable, reproducible are studied which include effects illumination, sample edges, stirring. dependence...
This study is an extension of in situ hybridization experiments showing expression mRNA encoding CHIP28 selected epithelial or endothelia spleen, colon, lung, and eye (H. Hasegawa, R. Zhang, A. Dohrman, S. Verkman. Am. J. Physiol. 264 (Cell 33): C237-C245, 1993). Additional tissues from rat were screened by hybridization, humans stained with a polyclonal anti-CHIP28 antibody. Northern blot showed the 2.8-kilobase kidney, heart. In strong cells choroid plexus, iris, ciliary body, lens...
In searching for a basolateral membrane water transporter in rat kidney with homology to channel forming integral protein (CHIP28), channel-collecting duct (WCH-CD), and mercurial-insensitive (MIWC), we cloned new member of the major intrinsic family (GLIP, -cero1Intrinsic B o - tein).GLIP cDNA had an 865-base pair open reading frame encoding 30.6-kDa 19-23% amino acid identity channels 36% bacterial glycerol facilitator GlpF.Northern blot analysis showed 6.6-kilobase mFWA GLIP kidney,...
The movement of water between the air space and capillary compartments is important for maintenance hydration during respiration reabsorption excess alveolar fluid. We have obtained immunocytochemical functional evidence that plasma-membrane channels are responsible transport in intact lung. Northern quantitative immunoblot analysis showed high expression CHIP28 (channel-forming integral membrane protein 28 kDa) rat lung; immunocytochemistry localization to epithelial cell plasma membranes....
The cystic fibrosis gene product (CFTR) is a complex protein that functions as an adenosine 3,5-monophosphate (cAMP)-stimulated ion channel and possibly regulator of intracellular processes. In order to determine whether the CFTR molecule contains functional aqueous pathway, anion, water, urea transport were measured in Xenopus oocytes expressing CFTR. Cyclic AMP agonists induced Cl - conductance 94 microsiemens increase water permeability 4 × 10 -4 centimeter per second was inhibited by...
The localization and transporting properties of a kidney protein homologous to human erythrocyte CHIP28 was evaluated. cDNA encoding rat CHIP28k isolated from renal cortex library. A 2.8-kb identified which contained an 807 bp open reading frame 28.8 kD with 94% amino acid identity CHIP28. in vitro translation rabbit reticulocyte lysate generated 28-kD protein; addition ER-derived microsomes gave 32-kD transmembrane glycoprotein. Translation truncated RNA demonstrated glycosylation residue...
A total of 31 patients with gastric cancer showing peritoneal dissemination received continuous hyperthermic perfusion (CHPP) in combination the administration cisplatin (CDDP) and mitomycin C (MMC). The authors developed a new special device named cavity expander (PCE) for sufficient direct temperature measurement cavity. As complications CHPP three presented bone marrow suppressions (leukocytes ⩽ 3000/mm3 and/or platelets 30,000/mm3): one, leakage intestinal anastomosis; perforation; acute...
The tissue distribution of mRNA encoding rat kidney water channel CHIP28k was determined by in situ hybridization. cDNA isolated homology to human erythrocyte CHIP28 (G. M. Preston and P. Agre. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991) used construct 155-base 35S-labeled cRNA sense antisense probes corresponding base pair 7-162. Fixed frozen tissues were cut 6- 12- microns sections, hybridized with at 55 degrees C for 16 h, exposed 5-9 days. In renal cortex, detected intensely on...
Abstract Background Despite recent advances in immunosuppressive therapy for patients with primary nephrotic syndrome, its effectiveness and safety have not been fully studied nationwide real-world clinical data Japan. Methods A 5-year cohort study, the Japan Nephrotic Syndrome Cohort Study, enrolled 374 syndrome 55 hospitals Japan, including 155, 148, 38, 33 minimal change disease (MCD), membranous nephropathy (MN), focal segmental glomerulosclerosis (FSGS), other glomerulonephritides,...
We investigated whether duodenal reflux through the pylorus is involved in development of gastric cancer. Male Wistar rats weighing 230–250 g were subjected to three types operative procedures: (i) allowing pylorus; (ii) a gastrojejunal stoma; and (iii) gastrotomy. No carcinogens given, animals killed 50 weeks after surgery. cancers detected any 18 with In contrast, seven (41%) 17 four (31%) 13 stoma had adenocarcinoma. Differences incidence between both groups gastrotomy group significant...
Simultaneous multiple gastric cancer was seen in 49 (5.8%) of 852 resected stomachs. Patients with cancers were older than those single (P < 0.01) and the incidence significantly higher male patients female 0.05). The rates carcinomas 9.6% early 4.0% advanced carcinomas. With regard to histologic type, rate differentiated type 71% undifferentiated 29%. In 68 serially stomachs, 5 (7.4%) new detected microscopically, whereas 4 (5.9%) had already been diagnosed grossly. carcinoma increased...
A new and better electrolyte for anodic oxidation of GaAs is described. It an aqueous solution tartaric or citric acid mixed with glycol can produce, in extremely stable manner, native oxide films remarkably improved dielectric properties.
The cloning and expression of an apical membrane water channel from rat kidney collecting duct (WCH-CD) homologous to a 28-kDa integral protein (CHIP28) was reported recently (K. Fushimi, S. Uchida, Y. Hara, Hirata, F. Marumo, Sasaki. Nature Lond. 361: 549-552, 1993). We obtained approximately 1.8-kilobase clone lambda gt10 cDNA library by polymerase chain reaction method; whereas the coding sequence (814 base pairs, predicted size 29 kDa) identical that reported, we identified in-frame ATG...
We assessed the expression profile of Mg(2+)-transporting molecules in obese diabetic rats as a cause hypermagnesiuric hypomagnesemia, which is involved development insulin resistance, hypertension, and coronary diseases. Kidneys were obtained from male Otsuka Long-Evans Tokushima fatty (OLETF) (LETO) at ages 16, 24, 34 wk. Expression profiles studied by real-time PCR immunohistochemistry together with measurements urine Mg(2+) excretion. Urine excretion was increased 24-wk-old OLETF...
Multicentric Castleman’s disease (MCD) is a benign lymphoproliferative disorder with heterogenous clinical symptoms, and involves systemic organs in addition to lymph nodes. Herein, we present the case of 55-year-old man MCD characterized by an extensive infiltration IgG4+ plasma cells kidneys. The patient presented our hospital high fever diarrhea. On admission, laboratory analysis revealed anemia, renal dysfunction (eGFR 30 mL/min/1.73 m2), polyclonal gammopathy (IgG 7130 mg/dL), elevated...
Activin A is known to impede tubular repair following renal ischemia, whereas exogenous follistatin, an activin antagonist, has been shown ameliorate kidney damage in rats. Despite these findings, the precise role of endogenous follistatin yet be elucidated. In this study, we investigated localization normal human and its potential utility as a marker for acute injury (AKI). total 118 AKI patients 16 healthy adults, levels serum urine were quantified using ELISA, correlations with clinical...
Multiphase phenomena in the application of in-mold electromagnetic stirring continuous casting were discussed through cold experiment using mercury and numerical simulation by two fluid model. The result revealed critical flow rate argon injected submerged entry nozzle, which leads to change pattern bulk liquid metal inside mold pool. This does not have a large effect on driven because Lorentz force acts mainly vicinity solidifying shell except for case amount rate. particle behavior under...