Oxidative stress and the resulting inflammation are essential pathological processes in acute lung injury (ALI). Nuclear factor erythroid 2-related 2 (Nrf2), a vital transcriptional factor, possesses antioxidative potential has become primary target to treat many diseases. Oridonin (Ori), isolated from plant Rabdosia Rrubescens, is natural substance that anti-inflammatory effects. Our aim was study whether antioxidant effects of Ori on LPS-induced ALI were mediated by Nrf2. MTT assays,...

6'-O-galloylpaeoniflorin (GPF), a galloylated derivative of paeoniflorin isolated from peony root, has been proven to possess antioxidant potential. In this present study, we revealed that GPF treatment exerted significant neuroprotection PC12 cells following OGD, as evidenced by reduction oxidative stress, inflammatory response, cellular injury, and apoptosis in vitro. Furthermore, with increased the levels phosphorylated Akt (p-Akt) nuclear factor-erythroid 2-related factor 2 (Nrf2), well...

Abstract Continued oxidative stress can lead to chronic inflammation, which in turn could mediate most diseases including cancer. Nuclear factor erythroid 2-related (Nrf2), a critical transcriptional activator for antioxidative responses, has envolved be an attractive drug target the treatment or prevention of human diseases. In present study, we investigated effects and mechanisms betulin on Nrf2 activation its involvement lipopolysaccharide (LPS)-triggered inflammatory system. macrophages,...

Lung carcinoma is the most common human cancer with poor prognosis and has an increasing incidence in recent years. However, related mechanism of lung onset not been completely explored. Piwi-interacting RNA (piRNA) a type noncoding small established function germ cells, interestingly, piRNA also shown to be implicated biology. In this study, piR-55490 was found silenced specimens cell lines, compared normal tissues cells. Intriguingly, expression level negatively associated patients'...

Among the cellular response mechanisms, nuclear factor erythroid-2 related 2 (Nrf2) pathway is considered a survival that alleviates oxidative injury, while both NLRP3 and NF-κB pathways are pro-inflammatory cause damage to cells. These implicated in development resolution of acute lung injury (ALI). Isoliquiritigenin, flavonoid from liquorice compound, suggested be regulator above pathways, but mechanisms how NLRP3/NF-κB interacts with Nrf2 its protective effects ALI remain unknown. In...

To investigate the effects of microRNA-7 (miR-7) on proliferation, migration and invasion non-small cell lung cancer NSCLC) cells by targeting FAK through ERK/MAPK signaling pathway.NSCLC tissues adjacent normal were obtained from 160 NSCLC patients after operation. lines (A549, H1299 H1355) a human fetal fibroblast line (MRC-5) obtained. assigned into miR-7 inhibitors, mimics, blank, mimics control, inhibitors siRNA + groups. The expressions mRNA in detected qRT-PCR Western-Blotting. Cell...

MicroRNAs (miRNAs or miRs) regulate gene expression at the posttranscriptional level and are involved in many biological processes such as cell proliferation migration, stem differentiation, inflammation, apoptosis. In particular, miR-144-3p is downregulated various cancers, its overexpression inhibits metastasis of cancer cells. However, role miR-144-5p non-small-cell lung (NSCLC), especially radiosensitivity, unknown. this study, we found that was NSCLC clinical specimens well lines...

Schematic model of the protective effects by Pts on LPS-induced lung fibrosis.

Isoliquiritigenin exerts protective effects on carrageenan-induced pleurisy and lung injury. The underlying mechanisms may be nearly involved in Nrf2-mediated antioxidative activities NLRP3/NF-κB-mediated anti-inflammatory activities.

Background and Purpose Our previous research showed that ferroptosis plays a crucial role in the pathophysiology of PM2.5‐induced lung injury. The present study aimed to investigate protective Nrf2 signalling pathway its bioactive molecule tectoridin injury by regulating ferroptosis. Experimental Approach We examined regulatory effect on Beas‐2b cells using Nrf2‐knockout (KO) mice siRNA transfection. effects underlying mechanisms were evaluated vitro vivo. Key Results deletion increased iron...

Purpose: A new type of polymeric micelle (PM) was assembled using a polyethylene glycol (PEG)-linked (PEGylated) amphiphilic copolymer and d-tocopheryl PEG 1000 succinate (TPGS ). The micelles were used to deliver doxorubicin (DOX) curcumin (CUR) for alleviating multidrug resistance (MDR) in lung cancer cells while enhancing the therapeutic efficacy DOX. Methods: Micelles loaded with DOX CUR film-forming technique. treat A549/Adr find out whether had ability reverse MDR cells. Some...

AMPK-mediated autophagy and Akt/mTOR pathways play important roles in current cancer treatments. Oridonin (Ori), an ent-kaurane diterpenoid isolated from Isodon rubescens, exerts extensive anti-tumor potential controversial effects on autophagy. In this study, we investigated the effect of Ori autophagy, apoptosis, AMPK/Akt/mTOR determined whether was related to increased cisplatin sensitivity observed A549 cells. First, found that suppressed Akt/mTOR, Bcl2 flux with enhanced levels Atg3,...

Zingerone possesses the potential to relieve asthma <italic>via</italic> upregulating AMPK/Nrf2/HO-1 signaling pathway.

Abstract Background Recent studies have shown 6'-O-galloylpaeoniflorin (GPF), a nature product extracted from the roots of paeoniflorin exerts anti-oxidant and anti-inflammatory activities. However, effects GPF on proliferation invasion in non-small cell lung cancer (NSCLC) cells not been clarified. Methods MTT assay was performed to determine cytotoxicity treatment NSCLC cells. Colony formation assay, scratch test transwell were vitro, respectively. An A549 xenograft mouse model confirm...

The development of hypertension involves extensive arterial wall remodeling, in which cysteine proteases play an essential role. Cathepsin L/V has been reported to be a protease that is closely intertwined with the tissue inflammatory response and extracellular matrix accumulation, allowing it regulate remodeling. aim this study was determine role cathepsin its regulatory pathway vascular remodeling hypertension. We showed endogenous inhibitor cystatin C, as well mitogen-activated protein...