A5046053082- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Osteoarthritis Treatment and Mechanisms
- Temporomandibular Joint Disorders
- Orthopaedic implants and arthroplasty
- Tissue Engineering and Regenerative Medicine
- Connective Tissue Growth Factor Research
- Alkaline Phosphatase Research Studies
- Obstructive Sleep Apnea Research
- 3D Printing in Biomedical Research
- Bone health and treatments
- Dental materials and restorations
- Bone Metabolism and Diseases
- dental development and anomalies
- Electrospun Nanofibers in Biomedical Applications
- Bone and Dental Protein Studies
- Advanced Sensor and Energy Harvesting Materials
- Advanced Materials and Mechanics
- Proteoglycans and glycosaminoglycans research
- TGF-β signaling in diseases
- Voice and Speech Disorders
- Polymer Surface Interaction Studies
- Bone and Joint Diseases
Okayama University
2016-2025
Pharmaceutical Biotechnology (Czechia)
2018-2021
Okayama Prefecture
2021
Case Western Reserve University
2019
Cornell University
2019
In-Q-Tel
2018
Regenerative Medicine Institute
2016
University of Dental Medicine
2013-2014
Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico
2012
Universidade de São Paulo
2012
MicroRNAs (miRNAs) are small RNA molecules of 21–25 nucleotides that regulate cell behavior through inhibition translation from mRNA to protein, promotion degradation and control gene transcription. In this study, we investigated the miRNA expression signatures cultures undergoing osteoblastic osteocytic differentiation mesenchymal stem cells (MSC) using mouse MSC line KUSA-A1 human MSCs. Ninety types were quantified during osteoblastic/osteocytic in utilizing PCR arrays. Coincidently with...
Abstract Whole-organ regeneration has great potential for the replacement of dysfunctional organs through reconstruction a fully functional bioengineered organ using three-dimensional cell manipulation in vitro . Recently, many basic studies whole-tooth have been conducted mouse model. Further evidence practical application to human medicine is required demonstrate tooth restoration by reconstructing germ postnatal large-animal Herein, we autologous transplantation canine The tooth, which...
Dental pulp cells (DPCs) are known to be enriched in stem/progenitor but not well characterized yet. Small non-coding microRNAs (miRNAs) have been identified control protein translation, mRNA stability and transcription, reported play important roles stem cell biology, related reprogramming, maintenance of stemness regulation differentiation. In order characterize dental its mechanism differentiation, we herein sorted stem-cell-enriched side population (SP) from human DPCs periodontal...
Current clinical procedures to control or regenerate bone loss due peri-implantitis are not predictable neither accomplish complete resolution. Therefore, early detection of the onset and active periods crucial for prevention extensive peri-implant resorption. This study aimed determine a possible association between presence collagenases (MMP-1, MMP-8 MMP-13) in sulcular fluid (PISF) by annually adjusted vertical (AVBL) measurements. Intended sample consisted 76 consecutive patients who...
During normal pulp tissue healing, inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) or interleukins, act in the initial 48 hours (inflammatory phase) and play important roles not only chemo-attractants of cells stem/progenitor but also inducing a cascade reactions toward regeneration reparative dentin formation both. Previous reports have shown that cytokines regulate differentiation capacity dental (DPCs), none has interrogated impact these on stem cell phenotype cells....
Inspired by the dynamic process of initial bone development, in which a soft tissue turns into solid load-bearing structure, fabrication, optimization, and characterization bioinduced variable-stiffness actuators that can morph various shapes change their properties from to rigid are hereby presented. Bilayer devices prepared combining electromechanically active polypyrrole with compliant behavior alginate gels uniquely functionalized cell-derived plasma membrane nanofragments (PMNFs),...
Ectopic bone marrow (eBM) holds tremendous potential as an artificial organ, serving not only in stem cell transplantation therapies but also a controlled experimental system for analyzing cellular dynamics and interactions between cells the matrix during formation, maintenance, aging of (BM). Although morphogenetic protein (BMP)-2 has been reported to induce eBM it remains unproven whether BMP-2 induced (BMP-eBM) can provide functional BM niche that is comparable native long bones (LB-BM)....
A deeper understanding of the detailed mechanism in vivo tissue healing is necessary for development novel regenerative therapies. Among several external factors, environmental pH one crucial parameters that greatly affects enzyme activity and cellular biochemical reactions involving repair homeostasis. In this study, order to analyze microenvironmental conditions during bone healing, we first measured at site using a high-resolution fiber optic microsensor directly femur defects tooth...
Bone marrow–derived mesenchymal stem/progenitor cells (BMSCs) are commonly used in regeneration therapy. The current primary source of BMSCs is the iliac crest; however, procedure associated with various burdens on patient, including risk pain and infection. Hence, possibility to collect from other, more accessible, sources would be an attractive approach. It well known that stem migrate surrounding tissues play important roles wound healing. We thus hypothesized could isolated granulation...
The pathology of medication-related osteonecrosis the jaw (MRONJ), often associated with antiresorptive therapy, is still not fully understood. Osteocyte networks are known to play a critical role in maintaining bone homeostasis and repair, but exact condition these MRONJ unknown. On other hand, local application E-coli-derived Recombinant Human Bone Morphogenetic Protein 2/β-Tricalcium phosphate (E-rhBMP-2/β-TCP) has been shown promote regeneration mitigate MRONJ-like mouse models,...
Summary The purposes of this study were to determine whether a response shift was observable after partial denture treatment and identify the predictors that influenced magnitude direction. A total 173 consecutive patients with no more than eight missing teeth who received implant‐supported, fixed or removable dentures at Okayama University Dental Hospital asked complete full‐version Oral Health‐Related Quality Life (OHRQoL) questionnaire before (pre‐test) (post‐test). Additionally, short...
ABSTRACT Articular cartilage repair remains a challenging problem. Based on high-throughput screening and functional analysis, we found that fluocinolone acetonide (FA) in combination with transforming growth factor beta 3 (TGF-β3) strongly potentiated chondrogenic differentiation of human bone marrow–derived mesenchymal stem cells (hBMSCs). In an vivo defect model knee joints immunocompromised mice, transplantation FA/TGF-β3–treated hBMSCs could completely the articular surface. Analysis...
Biomineralization involves complex processes and interactions between organic inorganic matters, which are controlled in part by the cells. The objectives of this study were, first, to perform a systematic ultrastructural investigation initial mineral formation during secondary ossification center mouse femur based on material science biology viewpoint, then develop novel biomaterials for mineralization vivo findings. First, we identified very deposition at postnatal day 5 (P5) medial side...
Mesenchymal stem cells (MSCs) are known to play important roles in the repair of lost or damaged tissues and immunotolerance. On other hand, aging is impair MSC function. However, little currently about how aged MSCs affect host response local inflammatory condition tissue deterioration periodontitis, which a progressive destructive disease periodontal potentially leading multiple tooth loss. In this study, we examined relationship between aging-induced impairment function severity...
In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate are still ineffective due some remaining gaps the understanding real vivo formation process, and application knowledge synthesis. Therefore, objectives this study were first, perform a systematic ultrastructural investigation initial mineral during intramembranous ossification mouse calvaria from material scientists' viewpoint, develop novel mineralization based on findings....
Medication-related osteonecrosis of the jaw (MRONJ) is a severe pathological condition associated mainly with long-term administration bone resorption inhibitors, which are known to induce suppression osteoclast activity and remodeling. Bone Morphogenetic Protein (BMP)-2 be strong inducer remodeling, by directly regulating osteoblast differentiation activity. This study aimed evaluate effects BMP-2 adsorbed onto beta-tricalcium phosphate (β-TCP), an osteoinductive bioceramic material allows...
Bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2) have been regarded as the major cytokines promoting bone formation, however, several studies reported unexpected results with failure of formation or resorption these factors. In this study, BMP-2 FGF-2 adsorbed into atellocollagen sponges were transplanted defects in marrow-scarce calvaria (extramedullary environment) marrow-abundant femur (medullary for analysis their vivo effects not only on osteoblasts,...