An unbalanced diet can have adverse effects on health. Long chain polyunsaturated fatty acids (LCPUFAs) been the focus of research owing to their necessity inclusion in a healthy diet. However, LCPUFAs human osteoclast formation and function not explored before. A CD14+ monocyte differentiation model was used elucidate an ω-3 LCPUFA, docosahexaenoic acid (DHA), ω-6 arachidonic (AA), activity. monocytes were isolated from peripheral blood donors stimulated with macrophage colony stimulating...

Osteoclasts are large, multinucleated cells that responsible for the breakdown or resorption of bone during remodelling. Studies have shown certain fatty acids (FAs) can increase formation, reduce loss, and influence total mass. Palmitoleic acid (PLA) is a 16-carbon, monounsaturated FA has anti-inflammatory properties similar to other FAs. The effects PLA in remain unexplored. Here we investigated on receptor activator nuclear factor kappa B (NF-κB) ligand (RANKL)-induced osteoclast...

Bone loss diseases are often associated with increased receptor activator of NF-κB ligand (RANKL)-induced osteoclast formation. Compounds that can attenuate RANKL-mediated formation great biomedical interest. Eugenol, a phenolic constituent clove oil possesses medicinal properties; however, its anti-osteoclastogenic potential is unexplored hitherto. Here, we found eugenol dose-dependently inhibited the RANKL-induced multinucleated and TRAP activity in RAW264.7 macrophages. The underlying...

Osteoclasts are the sole bone resorbing cell in body and their over activity is key development of osteoporosis. Osteoclastogenesis mediated by receptor activator nuclear factor κB ligand (RANKL) signalling pathways. Unsaturated fatty acids (UFA) known to inhibit osteoclastogenesis targeting RANKL signalling. However, mechanisms action remain unclear. Peroxisome proliferator activated receptors (PPARs) a family receptors, with three isoforms (PPAR-α, PPAR-β/δ PPAR-γ), that bind UFAs...

Bone is a dynamic tissue that undergoes continuous remodeling coupled with the action of osteoblasts and osteoclasts. Osteoclast activity elevated during osteoporosis periodontitis resulting in excessive loss trabecular alveolar bone. Osteoclasts are formed an inflammatory response to cytokine production receptor activator nuclear factor-kappaB (NF-κB) ligand (RANKL) bacterial challenge lipopolysaccharide (LPS). Carvacrol, monoterpenic phenol present Origanum vulgare Thymus vulgaris, natural...

Honeybush tea, a sweet tasting caffeine-free tea that is indigenous to South Africa, rich in bioactive compounds may have beneficial health effects. Bone remodeling physiological process involves the synthesis of bone matrix by osteoblasts and resorption osteoclasts. When exceeds formation, can be disrupted resulting diseases such as osteoporosis. Osteoclasts are multinucleated cells derived from hematopoietic precursors monocytic lineage. These fuse differentiate into mature osteoclasts...

Fermented rooibos extract has more potent inhibitory effect on osteoclasts than unfermented rooibos.

Osteoclasts are large, multinucleated cells that responsible for the resorption of bone. Bone degenerative diseases, such as osteoporosis, characterized by overactive osteoclasts. Receptor activator nuclear factor-κB (NF-κB) ligand (RANKL) binding to its receptor on osteoclast precursors will trigger formation and resorption. The production reactive oxygen species (ROS) is known play a crucial role in RANKL-induced G-protein coupled 120 (GPR120) signalling has been shown affect formation,...

The bromodomain ( BRD ) and extra‐terminal domain BET protein family bind to acetylated histones on lysine residues act as epigenetic readers. Recently, the role of this in bone loss has been gaining attention. Earlier studies have reported that benzotriazepine (Bzt) derivatives could be effective inhibitors proteins. In study, using silico tools we designed three Bzt analogs (W49, W51, W52). By docking, molecular simulations, chemiluminescent Alpha Screen binding assay, show studied were...

New Zealand is a rich source of food components that may have bioactivity on bone.Docosahexaenoic acid (DHA) from fish oil has been shown to maintain bone in ovariectomised (OVX) rats.Kiwifruit, fibre and carotenoids, also affect via prebiotic as well direct cell-based mechanisms.We aimed 1) ascertain the effects DHA two cell models, including interactions with soy isoflavones; 2) investigate specific carotenoids kiwifruit whole rodent models human study.RAW 264.7 mouse monocytes or marrow...

Bone remodelling in a healthy body is constant balance, maintaining an adaptive and robust skeletal system. In osteoporosis this balance disrupted with the rates of osteoclastic bone resorption exceeding osteoblastic formation, resulting lower mineral density. Rooibos tea, popular South African drink made from Aspalathus linearis leaves grown Western Cape. This tea rich phenolic compounds which have been widely investigated recent years as potential treatment for many ailments. study,...

Objective: Ensuring bone health is crucial for preventing conditions such as osteoporosis and fractures. Zingerone, a phytochemical compound, has gained attention its potential benefits in health. This study evaluated the osteoprotective of zingerone effects on differentiation signalling pathways vitro using SAOS-2 osteosarcoma RAW264.7 macrophage cell lines, aiming to elucidate mechanism action metabolism. Methods: cells were treated with at concentrations 0.1-200µM. Osteoblast...

Ensuring adequate bone health is crucial for preventing conditions such as osteoporosis and fractures. Zingerone, a phytonutrient isolated from cooked ginger, has gained attention its potential benefits in health. This study evaluated the osteoprotective of zingerone effects on differentiation signalling pathways

Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)

Alcohol intake at different developmental stages can lead to the development of alcohol-induced fatty liver disease (AFLD). Zingerone (ZO) possess hepato-protective properties; thus, when administered neonatally, it could render protection against AFLD. This study aimed evaluate potential long-term protective effect ZO One hundred and twenty-three 10-day-old Sprague–Dawley rat pups (60 males; 63 females) were randomly assigned four groups orally following treatment regimens daily during...

This study investigatedthe effects of eicosapentanoic acid and prostaglandin E₃ on osteoclast formation bone resorption in RAW 264.7 murine pre-osteoclasts.

Standardisation of techniques for bone cellular research Bone remodelling is a continuous process involving osteoclasts and osteoblasts. Long chain polyunsaturated acids (LCPUFA) are known to assist in disorders that disrupt the process. Methods study effects LCPUFAs on cells vitro have been standardized future studies.

Modulation of osteoclast formation and activity by phytoestrogens docosahexanoic acid in RAW264.7 murine monocytes This study was conducted to determine the vitro effects genistein daidzein together with on proliferation, osteoclastogenesis bone resorption monocytes. It seen that acid, phytoestrogens, have a protective effect vitro.

Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)

Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)