A5043538243- Veterinary Oncology Research
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Single-cell and spatial transcriptomics
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Inflammatory mediators and NSAID effects
- Effects and risks of endocrine disrupting chemicals
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Hedgehog Signaling Pathway Studies
- Lymphatic System and Diseases
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Cancer Genomics and Diagnostics
- Atherosclerosis and Cardiovascular Diseases
- Wnt/β-catenin signaling in development and cancer
- Advanced Breast Cancer Therapies
- Cancer, Stress, Anesthesia, and Immune Response
- Glycosylation and Glycoproteins Research
- Liver physiology and pathology
- Salivary Gland Tumors Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Melanoma and MAPK Pathways
The University of Tokyo
2014-2024
City of Hope
2019-2024
Okayama University of Science
2022-2024
Beckman Research Institute
2019-2024
Veterinary Medical Center
2015-2022
City Of Hope National Medical Center
2021
Kyushu University
2021
Weatherford College
2021
Hiratsuka City Hospital
1996
Abstract Background Intratumour heterogeneity is a hallmark of most solid tumours, including breast cancers. We applied spatial transcriptomics and single‐cell RNA‐sequencing on patient‐derived xenografts (PDXs) to profile spatially resolved cell populations within oestrogen receptor‐positive (ER + ) cancer elucidate their importance in oestrogen‐dependent tumour growth. Methods Two PDXs ‘ER‐high’ cancers with opposite oestrogen‐mediated growth responses were investigated:...
Abstract Menopause is a critical window of susceptibility for its sensitivity to endocrine disrupting chemicals due the decline endogenous estrogen. Using surgical menopausal (ovariectomized) mouse model, we assessed how mammary tissue was affected by both 17β-estradiol (E2) and polybrominated diphenyl ethers (PBDEs). As flame retardants in household products, PBDEs are widely detected human serum. During physiologically-relevant exposure E2, enhanced E2-mediated regrowth glands with...
The female mammary epithelium undergoes reorganization during development, pregnancy, and menopause, linking higher risk with breast cancer development. To characterize these periods of complex remodeling, here we report integrated 50 K mouse 24 human epithelial cell atlases obtained by single-cell RNA sequencing, which covers most lifetime stages. Our results indicate a putative trajectory that originates from embryonic stem cells differentiates into three lineages (basal, luminal...
Canine urothelial carcinoma (cUC) is the most common tumor of lower urinary tract in dogs. Although chemotherapy and radical surgery have improved overall survival, dogs with cUC succumb to metastasis or recurrence. Therefore, development an effective systematic therapy warranted. In this study, a comprehensive drug screening test using cell line was performed anti-tumor effect histone deacetylase (HDAC) inhibitor evaluated. Comprehensive on cells. Based screening, anti-proliferation...
Fibroblasts have been shown to be one of the essential players for mammary gland organization. Here, we identify two major types mouse fibroblasts through single-cell RNA sequencing analysis: Dpp4 + and - fibroblasts. Each population exhibits unique functional characteristics as well discrete localization in normal glands. Remarkably, estrogen, a crucial mediator organization, alters gene expression profiles population-specific manner, without distinct activation estrogen receptor signaling....
Transitional cell carcinoma (TCC) accounts for >90% of canine malignant tumours occurring in urinary bladder, and the prognosis is poor. Our previous study, using RNA sequencing, showed that human epidermal growth factor 2 (HER2) was most activated upstream regulator related to carcinogenesis TCC. The aim this study examine anti‐tumour effect lapatinib, a tyrosine kinase inhibitor HER2, on TCC lines vitro vivo. Five (TCCUB, Love, Sora, LCTCC, MCTCC) were used. Western blotting HER2...
Abstract Cancer-promoting inflammation is an important event in cancer development. Canine urothelial carcinoma (cUC) overexpresses prostaglandin E 2 (PGE ) and has a unique sensitivity to cyclooxygenase (COX2)-inhibiting therapy. In addition, majority of cUC harbour BRAF V595E mutation. However, mechanisms underlying aberrant PGE production patients remain unclear. Drug screening revealed that inhibition RAF/MEK/ERK pathway, p38 JNK pathway reduced cells. By pharmacological the multiple...
Canine anal sac gland carcinoma (ASGC) frequently occurs in the apocrine glands of canine and shows aggressive biological behavior. The expression human epidermal growth factor receptor 2 (HER2) has been reported various tumors. HER2 is a promising therapeutic target these tumors, HER2-targeted drugs, such as trastuzumab lapatinib, have improved outcome patients. In this study, ASGC was evaluated to investigate its potential for ASGC. mRNA surgically resected tissues significantly higher...
It is known that epithelial mesenchymal transition (EMT) contributes to the acquisition of malignant property in human cancers. However, role EMT canine tumors remains be elucidated. To evaluate correlation between expression levels protein markers involved and clinicopathological characteristics mammary gland tumors, immunohistochemistry using antibodies against ZO-1, E-cadherin, vimentin, N-cadherin fibronectin was performed on 119 clinical tissue samples. Consequently, loss ZO-1 gain...
The biological function of a nonstructural protein, NSm, Akabane virus (AKAV) is unknown. In this study, we generated series NSm deletion mutant viruses by reverse genetics and compared their phenotypes. in which the coding region was almost completely deleted could not be rescued, suggesting that plays role replication. We next possessing various partial deletions identified several regions critical for infectivity. All rescued produced smaller plaques grew inefficiently cell culture, to...
Podoplanin (PDPN), a small transmembrane mucin-like glycoprotein, is ectopically expressed. It also known to be linked with several aspects of tumor malignancy in some types human tumors, including invasion, metastasis, and cancer stemness. However, there are few reports on the expression dog PDPN (dPDPN) canine association between dPDPN has not been elucidated. We identified that 11 out 18 tumors expressed dPDPN. Furthermore, 80% malignant melanoma (MM), squamous cell carcinoma, meningioma...
The numbers of tumor infiltrating T lymphocytes, B lymphocytes and antigen presenting cells were evaluated in an immunohistochemical manner 140 canine spontaneous mammary gland (MGT) tissues. As a result, we found statistically significant increase the number intratumoral (23.2 ± 23.8) malignant MGT group (n=51) compared with benign (14.0 16.0, n=89; P<0.05). Moreover, high lymphocyte infiltration correlated poor prognosis multivariate analysis (P<0.05). This study indicated relationship...
Human epidermal growth factor receptor 2 (HER2) overexpression has been reported in various human cancers. HER2-targeted therapies showed clinical responses humans with HER2-positive tumors. The incidence of canine primary lung cancer (cPLC) is increasing, but there are no effective systemic for dogs late-stage cPLC. therapy could be an option cPLC, HER2 expression cPLC remains unknown. We evaluated Immunohistochemical analysis revealed that 3 samples (19%) scored 3+; 8 (50%), 2+; 5 (31%);...
Abstract Low‐dose cyclophosphamide ( CyLD ) has shown promise in the treatment of several cancers; however, effect on canine oral malignant melanoma never been explored. In this study, we investigated effects with or without piroxicam (Px) tumour neovascularization and vascular normalization a melanoma‐xenografted mice model. After , Px combination both CyPx ), growth groups was significantly suppressed compared to control group at 30 days treatment. Proliferation index also reduced by all...
A 12-year-old intact male Welsh Corgi was presented with enlargement of the right scrotum. Both testicles were surgically removed and histopathologically examined. On gross examination, white nodules found in epididymis ductus deferens. Histopathologically, developed continuously from tunica vaginalis testis scrotum consisted spindle-shaped neoplastic cells that invaded surrounding tissue. Immunohistochemically, diffusely positive for vimentin, cytokeratin Wilms tumor-1 (WT-1). Based on...
Urothelial carcinoma (UC) is the most common tumor affecting urinary bladder of dogs. Protein overexpression ErbB2 (the canine homolog HER2) has been observed in dogs with UC. However, no study regarding copy number aberration (CNA) reported In this study, a digital PCR assay for detecting CNA was developed. DNA samples were isolated from 83 formalin-fixed, paraffin-embedded tissues (36 UC, 8 polypoid cystitis, and 39 normal) 94 sediments (54 30 nonneoplastic, 10 normal). The chromosome (...
The human epidermal growth factor receptor 2 (HER2) is expressed in various cancers including thyroid (TC) and used as a diagnostic marker therapeutic target. Canine TC (cTC), the most common endocrine malignancy dogs, shows high metastasis rate, HER2-targeted therapy could be candidate for treatment. Here, we immunohistochemically evaluated HER2 expression 21 paraffin-embedded cTC tissues scored degree of based on intensity positivity (score: 0-3+). Four samples (19%) 3+; 6 (29%), 2+; 7...
Abstract Canine pulmonary adenocarcinoma (PAC) resembles human lung tumors in never-smokers, but it is rarer than adenocarcinoma. Therefore, research on canine PAC challenging. In the present study, we successfully established various novel cell lines from a single lesion dog, including two parent and fourteen cloned lines, characterized their cellular properties vitro. Several of these showed epithelial–mesenchymal transition (EMT)-like and/or cancer stem (CSCs)-like phenotypes. We...