A5010946256- Bone health and treatments
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Parathyroid Disorders and Treatments
- Bone and Joint Diseases
- Muscle Physiology and Disorders
- TGF-β signaling in diseases
- Vitamin D Research Studies
- Protease and Inhibitor Mechanisms
- Neuroendocrine Tumor Research Advances
- Heterotopic Ossification and Related Conditions
- Growth Hormone and Insulin-like Growth Factors
- Adipose Tissue and Metabolism
- Medical Imaging and Pathology Studies
- Spaceflight effects on biology
- Alkaline Phosphatase Research Studies
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Pituitary Gland Disorders and Treatments
- Neuroblastoma Research and Treatments
- Muscle metabolism and nutrition
- Extracellular vesicles in disease
- Bone and Dental Protein Studies
- Exercise and Physiological Responses
- Carcinogens and Genotoxicity Assessment
Kindai University
2015-2024
Kobe University
2003-2012
Diabetes Australia
2011
Chubu Rosai Hospital
2010
Japan Organization of Occupational Health and Safety
2007-2008
Miwa Hospital
1984-2007
Zeus Consulting (Greece)
2007
Hattori Botanical Laboratory
2002
McGill University
1999-2001
Royal Victoria Hospital
2001
Although patients with type 2 diabetes (T2DM) have an increased risk of hip fracture, vertebral fracture (VF) and its association BMD are still unclear. We examined Japanese T2DM (161 men >50 yr 137 postmenopausal women) non-DM controls (76 622, respectively) by lateral spine radiography DXA at the lumbar (L), femoral neck (FN), radius (R). Logistic regression analysis adjusted for age, body mass index, L-BMD showed that presence was independent factor prevalent VFs in women (OR = 1.86, p...
Sarcopenia and osteoporosis have recently been noted for their relationship with locomotive syndrome increased number of older people. is defined by decreased muscle mass impaired function, which may be associated frailty. Several clinical data indicated that related to bone reduced fracture risk. Genetic, endocrine mechanical factors as well inflammatory nutritional states concurrently affect tissues metabolism. genes, including myostatin α-actinin 3, shown in a genome-wide association...
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder characterized by tumors of parathyroids, pancreatic islets, and anterior pituitary. The MEN1 gene encodes a nuclear protein called menin. In carriers inactivating mutations give rise to truncated product consistent with menin acting as tumor suppressor gene. However, the role in tumorigenesis its physiological functions are not known. Here, we show that inactivation antisense RNA antagonizes transforming growth...
Abstract Although the action of bone morphogenetic protein (BMP) on osteoblast differentiation has been extensively investigated, its effect osteoclast remains unknown. In present study, in vitro effects BMP-2 osteoclast-like cell formation and resorption were examined. (1–100 ng/ml) significantly stimulated by preexistent cells mouse cultures containing stromal cells, whereas it did not affect bone-resorbing activity isolated rabbit cells. When was added to unfractionated after degeneration...
Abstract The present study was performed to clarify the role of high calcium concentration and appearance mononuclear cells at resorptive site in bone remodeling. Our recent revealed that extracellular ([Ca2+]e) stimulated DNA synthesis osteoblastic MC3T3-E1 not only directly but also indirectly via monocytes. Human monocyte-conditioned medium (CM) significantly inhibited alkaline phosphatase (ALP) activity. In contrast, when monocytes were cultured [Ca2+]e concentrations (more than 3 mM),...
Menin, the product of multiple endocrine neoplasia type 1 (MEN1) gene, is required for commitment multipotential mesenchymal stem cells to osteoblast lineage, however, it inhibits their later differentiation (Sowa, H., Kaji, Canaff, L., Hendy, G.N., Tsukamoto, T., Yamaguchi, Miyazono, K., Sugimoto, and Chihara, K. (2003) J. Biol. Chem. 278, 21058–21069). Here, we have examined mechanism action menin in regulating using mouse bone marrow stromal ST2 MC3T3-E1 cell lines. In cells, reduced...
Peripheral quantitative computed tomography (pQCT) is useful for evaluating volumetric bone mineral density (vBMD) as well (BMD) of cortical and trabecular bones separately. Although PTH affects differently, the effects endogenous on vBMD geometry have not previously been examined with pQCT. We, therefore, investigated an excess a deficiency by employing dual-energy x-ray absorptiometry pQCT in 36 female patients primary hyperparathyroidism (hyper), nine idiopathic or postoperative...
PTH, via the PTH/PTH-related protein receptor type 1 that couples to both kinase A (PKA) and C (PKC) pathways, canonical Wnt-β-catenin signaling pathway play important roles in bone formation. In present study we have examined interaction between PTH Wnt pathways mouse osteoblastic MC3T3-E1 cells. dose- time-dependently increased concentrations of β-catenin. The PKA activator, forskolin, PKC phorbol 12-myristate-13-acetate, as well analog, [Nle8,18,Tyr34]human PTH-(3–34)amide, all β-catenin...
Multiple endocrine neoplasia type 1 (MEN1) is less well recognized in Asian countries, including Japan, than the West. The clinical features and optimal management of MEN1 have yet to be clarified Japan. aim this study was clarify Japanese patients with MEN1.We established a MEN group designated 'MEN Consortium Japan' 2008, asked physicians surgeons provide genetic information on they had treated. Of 680 registered patients, 560 were analysed.Clinical examined.Primary hyperparathyroidism,...
Journal Article Modification in Growth of Transplantable Rat Tumors Exposed to Friend Virus Get access Hiroshi Kobayashi, Kobayashi Laboratory Pathology, Cancer Institute, Hokkaido University School Medicine, Sapporo, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Fujiro Sendo, Sendo Toshikazu Shirai, Shirai Kaji, Kaji Takao Kodama, Kodama Saito JNCI: the National Volume 42, Issue 3, March 1969, Pages 413–419, https://doi.org/10.1093/jnci/42.3.413...
Chronic renal failure induces bone mineral disorders and sarcopenia. Skeletal muscle affects other tissues, including bone, by releasing myokines. However, the effects of chronic on interactions between remain unclear.We investigated muscle, myokines linking to using a mouse 5/6 nephrectomy (Nx) model. Muscle mass density (BMD) were analysed quantitative computed tomography 8 weeks after Nx.Nephrectomy significantly reduced in whole body (12.1% reduction, P < 0.05), grip strength (10.1%...
We have previously shown that the extracellular calcium-sensing receptor (CaR) is expressed in various bone marrow-derived cell lines and plays an important role stimulating their proliferation chemotaxis. It has also been reported CaR modulates matrix production mineralization chondrogenic cells. However, it remains unclear whether any regulating osteoblast differentiation. In this study, we found of mouse osteoblastic MC3T3-E1 cells was increased when were exposed to high calcium (2.8 3.8...
Transforming growth factor (TGF) β inhibits alkaline phosphatase (ALP) activity and mineralization in mouse osteoblastic MC3T3-E1 cells, whereas local administration of TGF-β stimulates bone formation vivo. We recently demonstrated that Smad3, a signaling molecule, promotes ALP cells. Moreover, the target disruption Smad3 is reported to cause decrease mineral density. These findings indicate plays an important role regulation formation. However, why effects on are different remains unknown....
Although the actions of GH on osteoblasts have been extensively investigated, its effects osteoclasts remain unknown. In present study, bone resorption and osteoclast differentiation were examined in vitro. Bovine (bGH; 1-100 ng/ml) significantly stimulated by preexistent stromal cell-containing mouse cell cultures, whereas it did not affect bone-resorbing activity isolated rabbit osteoclasts. When bGH was added to unfractionated cells after degeneration osteoclasts, concentration...
The mechanisms whereby the parathyroid hormone (PTH) exerts its anabolic action on bone are incompletely understood. We previously showed that inhibition of ERK1/2 enhanced Smad3-induced in osteoblasts. These findings suggested hypothesis changes gene expression associated with altered signaling brought about by an inhibitor would identify novel factors therefore performed a comparative DNA microarray analysis between empty vector-transfected mouse osteoblastic MC3T3-E1 cells and...
ABSTRACT The further development in research of bone regeneration is necessary to meet the clinical demand for reconstruction. Plasminogen a critical factor tissue fibrinolytic system, which mediates repair skin and liver. However, role system remains unknown. Herein, we investigated ectopic formation using plasminogen-deficient (Plg–/–) mice. Bone femur delayed Plg–/– mice, unlike that wild-type (Plg+/+) deposition cartilage matrix osteoblast were both decreased Vessel formation, macrophage...