A5103194839- Spaceflight effects on biology
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Sports injuries and prevention
- Muscle activation and electromyography studies
- Bone health and treatments
- Biochemical effects in animals
- Shoulder Injury and Treatment
- Sports Performance and Training
- Osteoarthritis Treatment and Mechanisms
- Diabetes Treatment and Management
- Orthopedic Surgery and Rehabilitation
- Bone health and osteoporosis research
- Gout, Hyperuricemia, Uric Acid
- Alkaline Phosphatase Research Studies
- Shoulder and Clavicle Injuries
- Heat shock proteins research
- Parathyroid Disorders and Treatments
- Extracellular vesicles in disease
- Vitamin D Research Studies
- Bone Metabolism and Diseases
- Bone and Joint Diseases
- High Altitude and Hypoxia
Kindai University
2021-2024
Yokohama City University Medical Center
2020-2024
Yokohama City University
2020-2024
Takeda (Japan)
2015-2022
Doshisha University
2019-2022
Jikei University School of Medicine
2015-2019
Japan Aerospace Exploration Agency
2014-2019
Tokyo Dental College
2015
Ube Frontier University
2010-2014
Osaka University
2007-2014
The effect of microgravity on skeletal muscles has so far been examined in rat and mice only after short-term (5–20 day) spaceflights. drawer system (MDS) program, sponsored by Italian Space Agency, for the first time aimed to investigate consequences long-term (91 days) exposure within International Station. Muscle atrophy was present indistinctly all fiber types slow-twitch soleus muscle, but slightly greater than that observed 20 days spaceflight. Myosin heavy chain analysis indicated a...
Aim To assess the efficacy and safety of fasiglifam 25 50 mg in J apanese patients with type 2 diabetes inadequately controlled by diet exercise. Methods This phase III , double‐blind, placebo‐controlled, multicentre study included 192 randomized to once‐daily treatment (n = 63) or 62) placebo 67) for 24 weeks. The primary endpoint was change from baseline glycated haemoglobin ( HbA1c ) at week 24. Results At 24, both groups had significantly reduced levels compared group (p < 0.0001)....
Chronic renal failure induces bone mineral disorders and sarcopenia. Skeletal muscle affects other tissues, including bone, by releasing myokines. However, the effects of chronic on interactions between remain unclear.We investigated muscle, myokines linking to using a mouse 5/6 nephrectomy (Nx) model. Muscle mass density (BMD) were analysed quantitative computed tomography 8 weeks after Nx.Nephrectomy significantly reduced in whole body (12.1% reduction, P < 0.05), grip strength (10.1%...
Effects of 3-month exposure to microgravity environment on the expression genes and proteins in mouse brain were studied. Moreover, responses neurobiological parameters, nerve growth factor (NGF) derived neurotrophic (BDNF), also evaluated cerebellum, hippocampus, cortex, adrenal glands. Spaceflight-related changes gene protein observed. Biological processes up-regulated related immune response, metabolic process, and/or inflammatory response. Changes cellular components involving microsome...
Matrix vesicles (MtVs) are one of the extracellular (EVs) secreted by osteoblasts. Although MtVs have a classically-defined function as an initiator ossification and recent findings suggest role for in regulation bone cell biology, effects on repair remain unclear. In present study, we employed collagenase-released EVs (CREVs) containing abundant from mouse CREVs were administered locally gelatin hydrogels to damaged sites after femoral defect mice. exhibited characteristics with diameter...
This study comprehensively describes the effects of various levels food reduction on a wide range toxicological parameters in dietary-optimized rats (fed with approximately 75% ad libitum consumption daily; 16 g and 22 g/day for females males, respectively) that has been established as nutritionally appropriate well-controlled animal model conducting toxicity studies. Toxicological parameters, including general condition, ophthalmology, clinical pathology anatomic pathology, were examined...
Hormonal changes in humans during spaceflight have been demonstrated but the underlying mechanisms are still unknown. To clarify this point thyroid and testis/epididymis, both regulated by anterior pituitary gland, analyzed on long-term space-exposed male C57BL/10 mice, either wild type or pleiotrophin transgenic, overexpressing osteoblast stimulating factor-1. Glands were submitted to morphological functional analysis. In thyroids, volumetric ratios between thyrocytes colloid measured. cAMP...
The effects of heat stress on the morphological properties and intracellular signaling innervated denervated soleus muscles were investigated. Heat was applied to rats by immersing their hindlimbs in a warm water bath (42°C, 30 min/day, every other day following unilateral denervation) under anesthesia. During 14 days experimental period, for total seven times promoted growth-related hypertrophy sham-operated attenuated atrophy muscles. In muscles, transcription ubiquitin ligase,...
Response of adductor longus (AL) muscle to gravitational unloading and reloading was studied. Male Wistar Hannover rats (5-wk old) were hindlimb-unloaded for 16 days with or without 16-day ambulation recovery. The electromyogram (EMG) activity in AL decreased after acute unloading, but that the rostral region even elevated during continuous unloading. EMG levels caudal gradually increased up 6th day, again. Approximately 97% fibers pure type I at beginning experiment. Mean percentage 61%...
The mineral deposits in rabbit articular cartilage induced by intra-articular injections of glucocorticoid were studied light and electron microscopy, using histochemical techniques x-ray-probe microanalysis. This study demonstrated that the consisted hydroxyapatite crystals. initial deposition crystals was seen around degenerating chondrocytes, where a halo-like pericellular space contained large amount electron-dense amorphous material. precipitation with low ratio calcium to phosphorus...
Hydroxyapatite crystal deposition in the articular cartilage of proximal femoral head was examined by using light and electron microscopy probe microanalysis. The hydroxyapatite significantly more frequent patients with osteoarthritis (40.7%, 22 54) than control subjects (6.0%, 3 50) (P less 0.001). Initial needle-like crystals always seen on or within electron-dense, amorphous material around degenerating hypertrophic chondrocytes. Matrix vesicles were rarely calcifying areas.
As the first step in evaluating possibility of low-temperature atmospheric plasma for clinical applications treatment rhabdomyosarcoma (RMS), we determined effects exposure on C2C12 myoblasts. The was generated through an electrical discharge argon gas. One minute every 24 h inhibited cell proliferation, whereas myoblast differentiation not affected. Plasma increased phosphorylation ERK and JNK at 30 min after exposure, but both decreased to less than control levels 1 4 exposure. percentage...
Humoral factors that are secreted from skeletal muscles can regulate bone metabolism and contribute to muscle-bone relationships. Although extracellular vesicles (EVs) play important roles in physiological pathophysiological processes, the of EVs repair have remained unclear. In present study, we investigated effects local administration muscle cell-derived on control streptozotocin-treated diabetic female mice. Muscle (Myo-EVs) were isolated conditioned medium mouse C2C12 cells by...
Gravity-dependent physical processes strongly affect the ability of elderly people to maintain musculoskeletal health by reducing muscle atrophy and increasing bone mineral density, thereby quality life. A need therefore exists identify molecules in system that are responsive gravitational loading establish an objective indicator for maintenance healthy systems. Here, we performed integrated assessment results soleus proteomic analyses three model mouse experiments under different gravity...
The main purpose of the present study was to investigate role(s) 25-kDa heat shock protein (HSP25) in regulation and integration myofibrillar Z-disc structure during down- or upregulation size rat soleus muscle fibers. Hindlimb unloading by tail suspension performed adult rats for 7 days, reloading allowed 5 days after termination suspension. Interaction HSP25 proteins, phosphorylation status, distribution, complex formation were investigated. Non- single-phosphorylated HSP25s generally...
The effects of 3 months spaceflight (SF), hindlimb suspension, or exposure to 2G on the characteristics neck muscle in mice were studied. Three 8-week-old male C57BL/10J wild-type exposed microgravity International Space Station mouse drawer system (MDS) project, although only one returned Earth alive. Housing a small MDS cage (11.6 × 9.8-cm and 8.4-cm height) and/or regular vivarium was also performed as ground controls. Furthermore, ground-based suspension by using animal centrifuge (n = 5...
Abstract The molecular mechanisms associated with spaceflight‐induced biological adaptations that may affect many healthy tissue functions remain poorly understood. In this study, we analyzed temporal changes in the serum proteome of six astronauts during prolonged spaceflight missions using quantitative comprehensive analysis performed data‐independent acquisition method mass spectrometry (DIA‐MS). All participated a mission for approximately 6 months and showed decreasing trend T ‐scores...