A5083715768- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Protein purification and stability
- Parkinson's Disease Mechanisms and Treatments
- Cutaneous lymphoproliferative disorders research
- RNA Interference and Gene Delivery
- Autism Spectrum Disorder Research
- Immune responses and vaccinations
- T-cell and Retrovirus Studies
- Alzheimer's disease research and treatments
- Phytochemical compounds biological activities
- Allergic Rhinitis and Sensitization
- Diabetes and associated disorders
- Psoriasis: Treatment and Pathogenesis
- Atherosclerosis and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Transgenic Plants and Applications
- Hepatitis B Virus Studies
- Asthma and respiratory diseases
- Influenza Virus Research Studies
University Hospital of Zurich
2013-2017
University of Zurich
2012-2017
Ag retention on follicular dendritic cells (FDCs) is essential for B cell activation and clonal selection within germinal centers. Protein deposited FDCs after formation of immune complexes with specific Abs. In this study, by comparing the same antigenic determinant either as soluble protein or virus-like particle (VLP), we demonstrate that VLPs are transported efficiently to murine splenic in vivo absence prior immunity. Natural IgM Abs complement were required sufficient mediate capture...
Abstract Monoclonal antibodies are widely used to treat non-infectious conditions but costly. Vaccines could offer a cost-effective alternative have been limited by sub-optimal T-cell stimulation and/or weak vaccine responses in recipients, for example, elderly patients. We previously shown that the repetitive structure of virus-like-particles (VLPs) can effectively bypass self-tolerance therapeutic vaccines. Their efficacy be increased even further incorporation an epitope stimulating T...
Abstract Extensive studies have been undertaken to describe naive B cells differentiating into memory at a cellular and molecular level. However, relatively little is known about the fate of upon Ag re-encounter. We previously established system based on virus-like particles (VLPs), which allows tracking VLP-specific by flow cytometry as well histology. Using allotype markers, it possible adoptively transfer mouse track responses in same under physiological conditions. observed that quickly...
Allergen-specific IgGs are known to inhibit IgE-mediated mast cell degranulation by two mechanisms, allergen-neutralization and engagement of the inhibitory FcγRIIB recruiting phosphatase SHIP-1. Here we unravel an additional mechanism IgG-mediated desensitization in mice: down-regulation allergen-specific IgE.Mast cells were loaded vitro vivo with monoclonal IgE antibodies specific for Fel d1 exposed immune complexes consisting d1-specific IgG recognizing different epitopes. Down regulation...
Although current influenza vaccines are effective in general, there is an urgent need for the development of new technologies to improve vaccine production timelines, capacities and immunogenicity. Herein, we describe technology which enables recombinant highly efficient bacterial expression systems. The globular head domain hemagglutinin, comprising most protein's neutralizing epitopes, was expressed E. coli covalently conjugated bacteriophage-derived virus-like particles produced...
Since the discovery of first virus-like particle (VLP) derived from hepatitis B virus in 1980 (1), field has expanded substantially. Besides successful use VLPs as safe autologous virus-targeting vaccines, powerful immunogenicity been also harnessed to generate immune response against heterologous and even self-antigens (2-4). Linking adjuvants displaying antigen ensures simultaneous delivery all vaccine components same antigen-presenting cells. As a consequence, cells, such dendritic will...
The lung is an important entry site for pathogens; its exposure to antigens results in systemic as well local IgA and IgG antibodies. Here we show that intranasal administration of virus-like particles (VLPs) splenic B-cell responses with strong germinal-center formation. Surprisingly, VLPs were not transported from the spleen a free form but by B cells. interaction between cells was initiated occurred independently complement receptor 2 Fcγ receptors, dependent upon receptors. Thus, passing...
Parkinson's disease (PD) is a progressive and currently incurable neurological disorder characterised by the loss of midbrain dopaminergic neurons accumulation aggregated alpha-synuclein (a-syn). Oligomeric a-syn proposed to play central role in spreading protein aggregation brain with associated cellular toxicity contributing decline. For this reason, oligomers have attracted interest as therapeutic targets for neurodegenerative conditions such PD other alpha-synucleinopathies. In addition...
Monoclonal antibodies (mAbs) inhibiting cytokines have recently emerged as new drug modalities for the treatment of chronic inflammatory diseases. Interleukin-17 (IL-17) is a T-cell-derived central mediator autoimmunity. Immunization with Qβ-IL-17, virus-like particle based vaccine, has been shown to produce autoantibodies in mice and was effective ameliorating disease symptoms animal models To characterize induced by vaccination at molecular level, we generated mouse mAbs specific IL-17...
Abstract Mycosis fungoides and its leukaemic counterpart Sézary syndrome are the most frequent cutaneous T‐cell lymphomas ( CTCL ), there is no cure for these diseases. We evaluated effect of clinically approved antihistamines on growth cell lines. lines as well blood lymphocytes from patients with were cultured antihistamines, analysed proliferation, apoptosis expression programmed death molecules transcription factors. The two clemastine desloratadine, currently used symptom alleviation in...
Several memory B-cell subclasses with distinct functions have been described, of which the most effective is class-switched (CS) population. We previously shown, using virus-like particles (VLPs), that proliferative potential these CS B cells limited and they fail to re-enter germinal centres (GCs). However, VLP-specific quickly differentiated into secondary plasma (PCs) virtue elevated antibody production compared primary PCs. Whereas induction VLP+ was strongly dependent on T helper cells,...