A5030224172- Genomics and Chromatin Dynamics
- Hemoglobinopathies and Related Disorders
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Advanced Fluorescence Microscopy Techniques
- DNA and Nucleic Acid Chemistry
- Chromatin Remodeling and Cancer
- Advanced biosensing and bioanalysis techniques
- Acute Myeloid Leukemia Research
- Innovation and Socioeconomic Development
- Luminescence and Fluorescent Materials
- Insect symbiosis and bacterial influences
- Molecular Biology Techniques and Applications
- FOXO transcription factor regulation
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
Peking University
2006-2024
National Institute of Biological Sciences, Beijing
2024
Tsinghua University
2024
Center for Life Sciences
2021-2024
Janelia Research Campus
2013-2016
Howard Hughes Medical Institute
2013-2016
Children's Hospital of Philadelphia
2008-2014
University of Pennsylvania
2008-2013
Helix (United States)
2013
Philadelphia University
2010
Genome editing with a Cas9 scalpel The nuclease forms the heart of CRISPR-Cas genome system. binds small guide RNAs that direct it to its target sites, where either cleaves or genomic DNA. Knight et al. used single-molecule imaging track in living cells. searches for sites using rapid threedimensional diffusion. It spends very little time binding off-target which explains high accuracy CRISPRCas9 machine. Science , this issue p. 823
Significance We have derived a new technology for the detection of genes within undisturbed nuclei fixed cells and tissues. Previous approaches used fluorescent DNA probes to hybridize interest, requiring treatment heat disruptive chemicals that distort natural organization nucleus. Instead, we bacterial protein, CRISPR (clustered regularly interspaced short palindromic repeats), combined with an RNA sequence as find interest in intact genome. This approach preserves spatial relationships...
Acetylation of histones triggers association with bromodomain-containing proteins that regulate diverse chromatin-related processes. Although acetylation transcription factors has been appreciated for some time, the mechanistic consequences are less well understood. The hematopoietic factor GATA1 is acetylated at conserved lysines required its stable chromatin. We show BET family protein Brd3 binds via first bromodomain (BD1) to in an acetylation-dependent manner vitro and vivo. Mutation a...
The transcription factor GATA1 regulates an extensive program of gene activation and repression during erythroid development. However, the associated mechanisms, including contributions distal versus proximal cis -regulatory modules, co-occupancy with other factors, effects histone modifications, are poorly understood. We studied these problems genome-wide in a Gata1 knockout erythroblast cell line that undergoes GATA1-dependent terminal maturation, identifying 2616 GATA1-responsive genes...
RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA + ) ATPase RUVBL2 co-occupies promoters Pol and various transcription factors. interacts unphosphorylated in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering initiation. Rapid depletion of leads a decrease number inhibits nascent...
Insect transmission is an essential process of infection for numerous plant and animal viruses. How insect-transmissible virus enters insect cell to initiate the cycle poorly understood, especially nonenveloped The capsid protein P2 rice dwarf (RDV), which nonenveloped, necessary transmission. Here, we present evidence that shares structural features with membrane-fusogenic proteins encoded by enveloped When RDV was ectopically expressed displayed on surface Spodoptera frugiperda cells, it...
Pioneer transcription factors (PTFs) possess the unique capability to access closed chromatin regions and initiate cell fate changes, yet underlying mechanisms remain elusive. Here, we characterized single-molecule dynamics of PTFs targeting in living cells, revealing a notable "confined target search" mechanism. like FOXA1, FOXA2, SOX2, OCT4 KLF4 sampled more frequently than non-pioneer factor MYC, alternating between fast free diffusion nucleus slower confined within mesoscale zones....
Yeast two‐hybrid and coimmunoprecipitation assays indicated that P8, an outer capsid protein of Rice dwarf phytoreovirus (RDV), interacts with rice glycolate oxidase (GOX), a typical enzyme peroxisomes. Confocal immunofluorescence microscopy revealed P8 was colocalized GOX in Time course analysis demonstrated the localization Spodoptera frugiperda cells changed from diffuse to discrete, punctuate inclusions during expression 24 48 h post inoculation. Coexpression may target into peroxisomes,...
Organic fluorophores are the keystone of advanced biological imaging. The vast chemical space has been extensively explored in seek molecules with ideal properties. However, within current molecular constraints, there appears to be a trade-off between high brightness, robust photostability, and tunable biochemical Herein we report general strategy systematically boost performance donor-acceptor-type by leveraging SO2 O-substituted azabicyclo[3.2.1] octane auxochromes. These bicyclic...
Distal enhancers physically contact target promoters to confer high level transcription. At the mammalian β-globin loci long-range chromosomal interactions between a distal enhancer, called locus control region (LCR), and globin genes are developmentally dynamic such that LCR loops embryonic, fetal adult in stage-appropriate fashion. LCR-globin gene require nuclear factor Ldb1. Recently, we employed artificial zinc finger (ZF) proteins Ldb1 endogenous force an LCR-promoter loop. This led...