A5004784857- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Cellular transport and secretion
- Metabolism, Diabetes, and Cancer
- Pharmacological Effects and Assays
- Sleep and Wakefulness Research
- Vagus Nerve Stimulation Research
- Pluripotent Stem Cells Research
- Diabetes Treatment and Management
- Neuroscience and Neuropharmacology Research
- Diabetes and associated disorders
- Calcium signaling and nucleotide metabolism
- Diabetes Management and Research
- Pancreatitis Pathology and Treatment
- Cannabis and Cannabinoid Research
- Amyotrophic Lateral Sclerosis Research
- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Anesthesia and Neurotoxicity Research
- Histone Deacetylase Inhibitors Research
- Pharmacological Receptor Mechanisms and Effects
- Nicotinic Acetylcholine Receptors Study
Karolinska Institutet
2011-2024
Northeast Normal University
2015-2023
Karolinska University Hospital
2005-2023
Friedrich Schiller University Jena
2021
Jena University Hospital
2021
Tianjin Medical University
2010-2011
University of California, Berkeley
1999
First Bethune Hospital of Jilin University
1992
Selective Induction of LTP and LTD by Postsynaptic [Ca 2+ ] i Elevation. Long-term potentiation (LTP) long-term depression (LTD), two prominent forms synaptic plasticity at glutamatergic afferents to CA1 hippocampal pyramidal cells, are both triggered the elevation postsynaptic intracellular calcium concentration ([Ca ). To understand how one signaling molecule can be responsible for triggering opposing modulation, different patterns were generated a new caged compound nitrophenyl-ethylene...
A decrease in islet β-cell mass is closely associated with the development and progression of diabetes.Therefore, protection against loss an essential measure to prevent treat diabetes.In this study, we investigated protective effects non-photoactivated hypericin, a natural compound, on β-cells both vitro vivo.In vitro, hypericin greatly improved INS-1 cell viability under high-glucose high-fatty-acid conditions by inhibiting glucotoxicity-and lipotoxicity-induced apoptosis nitric oxide (NO)...
Insulinomas cause neuroglycopenic symptoms, permanent neurological damage and even death. Current available therapies cannot satisfactorily treat malignant insulinomas some benign insulinomas. The promising phototherapeutic results harmless side effects of hypericin in cancer treatments prompted us to explore possible anti-growth activity photoactivated against RINm5F insulinoma cells underlying mechanisms. We now show that detectable maximal internalization occurred 20 60 min respectively....
The decreased expression and dysfunction of glucose transporter 4 (GLUT4), the insulin-responsive transporter, are closely related to occurrence insulin resistance (IR).To improve GLUT4 may represent a promising strategy prevent treat IR type 2 diabetes (T2DM).Here, we demonstrate that natural compound tectorigenin (TG) enhances expression, uptake responsiveness via activating AMP-activated protein kinase (AMPK)/myocyte enhancer factor (MEF2) signaling in both normal skeletal muscle cells...
Voltage-gated Ca2+ channels (Cav) are essential for pancreatic beta cell function as they mediate influx, which leads to insulin exocytosis. The β3 subunit of Cav (Cavβ3) has been suggested regulate cytosolic ([Ca2+]i) oscillation frequency and secretion under physiological conditions, but its role in diabetes is unclear. Here, we report that islets from diabetic mice show Cavβ3 overexpression, altered [Ca2+]i dynamics, impaired upon glucose stimulation. Consequently, high-fat diet...
Abstract In human mesenchymal stem cells (hMSCs), toll-like receptor 3 (TLR3) and TLR4 act as key players in the tissue repair process by recognizing their ligands stimulating downstream processes including cytokine release. The mechanisms of TLR3- TLR4-mediated releases from hMSCs remain uncertain. Here, we show that exposure to TLR3 agonist polyinosinic-polycytidylic acid (poly(I:C)) or incubation with lipopolysaccharide (LPS) increased mRNA expression levels TLR3, cytokines hMSCs....
Insulin secretion is critically dependent on the proper function of a complex molecular network. CaV2.3-knockout (CaV2.3–/–) and PKCλ-knockout (PKCλ–/–) mouse models now suggest that these 2 players, Cav2.3 channel PKCλ, are important constituents this Subsequent to glucose stimulation, insulin released from pancreatic β cell in biphasic pattern, i.e., rapid initial phase followed by slower, more sustained phase. Interestingly, Ca2+ influx through CaV2.3 regulates only second secretion. PKCλ...
Significance We reveal that increased expression of Ca V 3.1 channels in rat islets selectively impairs first-phase glucose-stimulated insulin secretion. This deterioration is recapitulated human islets. Its causal role diabetes development clearly manifested an vivo diabetic model. Mechanistically, this due to reduction phosphorylated FoxO1 the cytoplasm, elevated nuclear retention, and decreased syntaxin 1A, SNAP-25, synaptotagmin III a channel- calcineurin-dependent manner. Our findings...
Patients with amyotrophic lateral sclerosis (ALS) often show hallmarks of type 2 diabetes mellitus (T2DM). However, the causal link between ALS and T2DM has remained a mystery. We now demonstrate that 60% patients (ALS-T2DM) have sera exaggerated K+-induced increases in cytosolic free Ca2+ concentration ([Ca2+]i) mouse islet cells. The effect was attributed to presence pathogenic immunoglobulin Gs (IgGs) ALS-T2DM sera. IgGs immunocaptured voltage-dependent (CaV) channel subunit CaVα2δ1...
Multiple inositol polyphosphate phosphatase (MINPP1) is an enigmatic enzyme that responsible for the metabolism of hexakisphosphate (InsP6) and 1,3,4,5,6 pentakisphosphate (Ins(1,3,4,5,6)P5 in mammalian cells, despite being restricted to confines ER. The reason this compartmentalization unclear. In our previous studies insulin-secreting HIT cell line, we expressed MINPP1 cytosol artificially reduce concentration these higher phosphates. Undocumented at time, noted cytosolic expression...
Glucotoxicity or lipotoxicity leads to hyperglycemia and insulin secretion deficiency, which are important causes for the onset of type 2 diabetes mellitus (T2DM). Thus, restoration β-cell function is a long-sought goal in research. Previous studies have implicated pancreatic duodenal homeobox 1 gene (Pdx1) secretion. In this study, we established Pdx1 promoter-dependent luciferase system identified natural compound dracorhodin perchlorate (DP) as an effective promotor expression. We further...
Both the serine/threonine protein kinase Akt and voltage-gated L-type calcium channel act as important players in glucose-stimulated insulin secretion. recruits to maintains them plasma membrane. This study aimed characterize role of channels mediation signaling secretion.Insulin secretion was evaluated rat pancreatic islets INS-1 β cells by a standard radioimmunoassay.Akt inhibition effectively abrogates not only but also potassium depolarization-stimulated from islets, latter critically...
We exploited the anterior chamber of eye (ACE) immunodeficient mice as an ectopic site for both transplantation and microimaging engineered surrogate islets from human induced pluripotent stem cells (hiPSC-SIs). These contained a majority insulin-expressing cells, positive or negative PDX1 NKX6.1, minority glucagon- somatostatin-positive cells. Single, non-aggregated hiPSC-SIs were satisfactorily engrafted onto iris. They underwent gradual vascularization progressively increased their light...
CaV3 channels are ontogenetically downregulated with the maturation of certain electrically excitable cells, including pancreatic β cells. Abnormally exaggerated drive dedifferentiation mature This led us to question whether excessive channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) act as an obstacle hiPSC-islet maturation. We addressed this by using anterior chamber eye (ACE) immunodeficient mice a site for recapitulation vivo...
NEURONAL c-Fos protein-like immunoreactivity (c-Fos IR) in parts of the nucleus tractus solitarii (NTS)-dorsal motor vagus nerve (dmnX) complex anaesthetized rat was studied 90 min after NTS micro-injections neuropeptide Y (NPY) and/or adrenergic receptor agonists relevant cardiovascular doses. Microinjections NPY and l-adrenaline alone into induced neuronal IR NTS-dmnX complex, while clonidine had no action. No doublelabelled GFAP/c-Fos immunoreactive cells were found microinjection NTS....