A5087122395- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Mast cells and histamine
- Asthma and respiratory diseases
- Drug-Induced Adverse Reactions
- Parasites and Host Interactions
- Immune Cell Function and Interaction
- Urticaria and Related Conditions
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Viral gastroenteritis research and epidemiology
- Respiratory and Cough-Related Research
- Microscopic Colitis
- Pediatric health and respiratory diseases
- Adrenal Hormones and Disorders
- Dermatology and Skin Diseases
- Interpreting and Communication in Healthcare
- Fibromyalgia and Chronic Fatigue Syndrome Research
- HIV Research and Treatment
- Migration, Health and Trauma
- Biochemical Analysis and Sensing Techniques
- Escherichia coli research studies
- Nasal Surgery and Airway Studies
- Clostridium difficile and Clostridium perfringens research
University of North Carolina at Chapel Hill
2016-2025
Massachusetts General Hospital
2007-2023
Center for Rheumatology
2009
Harvard University
2004-2006
Importance An estimated 7.6% of children and 10.8% adults have IgE-mediated food-protein allergies in the US. food may cause anaphylaxis death. A delayed, allergic response to food-carbohydrate galactose-α-1,3-galactose (alpha-gal) mammalian meat affects an 96 000 450 individuals US is currently a leading food-related adults. Observations In US, 9 foods account for more than 90% allergies—crustacean shellfish, dairy, peanut, tree nuts, fin fish, egg, wheat, soy, sesame. Peanut fatal...
Abstract Introduction Alpha‐gal syndrome (AGS) is characterized by delayed hypersensitivity to non‐primate mammalian meat in people having specific immunoglobulin E (sIgE) the oligosaccharide galactose‐alpha‐1,3‐galactose. AGS has been linked tick bites from Amblyomma americanum ( Aa ) U.S. A small animal model of allergy needed study mechanism alpha‐gal sensitization, effector phase leading allergic responses and potential therapeutics treat AGS. Methods Eight‐ ten‐weeks old mice with a...
Abstract Whether epigenetic factor UTX, a histone H3 lysine 27 (H3K27) demethylase, is critical for type 2 immunity, including allergic sensitization and antigen-driven anaphylaxis, unclear. We used UTXfl/fl x Lck-Cre mice with UTX-deficient T cells (UTX-TCD) to determine whether cell-specific UTX expression regulates antigen-specific IgE production after airway peanut anaphylaxis following intraperitoneal (i.p.) challenge. UTX-TCD sensitized via the lipopolysaccharide (LPS), bacterial...
Abstract The impact of endemic parasitic infection on vaccine efficacy is an important consideration for development and deployment. We have examined whether intestinal with the natural murine helminth Heligmosomoides polygyrus bakeri alters Ag-specific Ab cellular immune responses to oral parenteral vaccination in mice. Oral mice a clinically relevant, live, attenuated, recombinant Salmonella expressing chicken egg OVA (Salmonella-OVA) induced accumulation activated, OVA-specific T effector...
The requirement for TLR signaling in the initiation of an Ag-specific Ab response is controversial. In this report we show that a novel OVA-expressing recombinant Salmonella vaccine (Salmonella-OVA) elicits Th1-biased cell-mediated and serum upon oral or i.p. immunization C57BL/6 mice. MyD88(-/-) mice, Th1-dependent responses are greatly reduced while Th2-dependent isotypes elevated to i.p., but not s.c. footpad, immunization. When T effector vaccination examined find activated, adoptively...
The mechanisms of pathogenesis driving alpha-gal syndrome (AGS) are not fully understood. Differences in immune gene expression between AGS individuals and non-allergic controls may illuminate molecular pathways targets critical for development. We performed profiling with RNA from the peripheral blood mononuclear cells (PBMCs) seven controls, 15 participants, two participants sensitized but allergic to using NanoString nCounter PanCancer panel, which includes 770 genes 14 different cell...
Background 10% of US residents have food allergies, including 2% with peanut allergy. Mast cell mediators released during the allergy effector phase drive allergic reactions. Therefore, targeting sensitized mast cells may prevent symptoms. Objective We used novel, human, allergen-specific, IgE monoclonal antibodies (mAbs) created using human hybridoma techniques to design an in vitro system evaluate potential therapeutics cells. Methods Two mAbs specific for peanut, generated through...