Introduction Enterotoxigenic Escherichia coli (ETEC) is the main diarrhea-causing pathogen in children and young animals has become a global health concern. Berberine type of “medicine food homology” long history use China, particularly treating gastrointestinal disorders bacterial diarrhea. Methods In this study, we explored effects berberine on growth performance, intestinal inflammation, oxidative damage, microbiota weaned piglet model ETEC infection. Twenty-four piglets were randomly...

Introduction Enterotoxic Escherichia coli (ETEC) is the main pathogen that causes diarrhea, especially in young children. This disease can lead to substantial morbidity and mortality a major global health concern. Managing ETEC infections challenging owing increasing prevalence of antibiotic resistance. Berberine, categorized as substance with similarities “medicine food,” has been used China for hundreds years treat gastrointestinal disorders bacteria-induced diarrhea. study investigated...

The enantioselective ring-opening reactions of methylenecyclopropanes (MCPs) involving C–C bond activation via oxidative addition transition metals have been rarely reported. Here, we disclose a Pd/Cu-catalyzed enantio- and regioselective coupling between cyclic imino esters MCPs to produce α-allylated 2H-pyrrole derivatives. In this reaction, azomethine ylide formed by chiral copper catalyst with ketimine ester would serve as nucleophile react activated palladium catalysis. This bimetallic...

Identifying individuals with target mutant phenotypes is a significant procedure in exploitation for implementing genome editing technology crop breeding programme. In the present study, rapid and non-invasive method was proposed to identify CRISPR/Cas9-induced rice mutants from their acceptor lines (huaidao-1 nanjing46) using hyperspectral imaging near-infrared (NIR) range (874.41-1733.91 nm) combined chemometric analysis. The data were analysed principal component analysis (PCA)...

An anti‐cancer peptide was purified from the Mercenaria ( Meretrix meretrix Linnaeus) by method of chromatography on Sephadex G‐25 and FPLC, its molecular weight determined to be 3147 Da way MALDI‐TOF mass spectrum. The effects this human gastric gland carcinoma cells (BGC‐823) their cytoskeletal morphology were investigated. results showed that could inhibit proliferation BGC‐823 obviously destroy skeletal structures cells. When concentration reached 4.0 μg/ml, inhibition percentage cell...

Celery (Apium graveolens L.) is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting celery molecular breeding are quite limited, thus few studies on have been conducted so far. In this study we made use simple sequence repeat (SSR) markers generated from previous transcriptome sequencing attempted to detect diversity relationships commonly used accessions explore efficiency primers for cultivars identification. Analysis variance (AMOVA)...

Selenium, in the form of selenocysteine, is a critical component some major redox-regulating enzymes, including thioredoxin reductase (TrxR) and glutathione peroxidase (Gpx). TrxR has emerged as an anticancer target for drug development due to its elevated expression level many aggressive human tumors. Acylfulvenes (AFs) are semisynthetic derivatives natural product illudin S display improved cytotoxic selectivity profiles. AF alkylate cellular macromolecules. Compared AFs, more readily...

A Cu-catalyzed coupling of cyclic imino esters with 2H-azirines has been developed to synthesize novel optically active aziridines in high yields excellent levels diastereo- and enantioselectivities under mild conditions. This protocol features a broad substrate scope good functional group compatibility, it enriches the existing reaction type rapid synthesis bearing vicinal tetrasubstituted stereogenic carbon centers.

Acylfulvenes (AFs) are a class of antitumor agents with favorable cytotoxic selectivity profiles compared to their natural product precursor, illudin S. Like many alkylating agents, S and AFs readily react thiol-containing small molecules such as cysteine, glutathione cysteine-containing peptides; reduced cellular levels can affect toxicity. Glutathione reductase (GR) is critical antioxidant enzyme that regulates the intracellular ratio reduced–oxidized glutathione. In this study, we found...

Panax ginseng C.A.Meyer is one of the most valuable traditional Chinese medicines. In this study, essential oil leaves (EOGL), collected using hydrodistillation and analyzed by GC/MS, contained a complex mixture aliphatic (69.0%), terpenoid (21.5%) aromatic compounds (2.4%). Among 54 components identified, major ones were palmitic acid (36.1%), β-farnesene (15.4%), linoleic (9.8%) phytol (5.6%). cytotoxicity EOGL exhibited obvious cytotoxic activities against different cancer cell lines,...

The serine hydrolases constitute a large class of enzymes that play important roles in physiology. There is great interest the development potent and selective pharmacological inhibitors these proteins. Traditional active-site often have limited selectivity within this superfamily are tedious expensive to discover. Using hydrolase RBBP9 as model target, we designed rapid relatively inexpensive route highly peptoid-based can be activated by visible light. This technology provides access...

Traditional Chinese medicine (TCM) has long been demonstrated to exert a therapeutic effect on various diseases and used as substitute for antibiotics in pig production. However, few studies have investigated the relationship between intestinal microbiota apparent nutrient digestibility when weaned piglet diets are supplemented with TCM. One hundred sixty-two 25-day-old weaning piglets were housed an environmentally controlled nursery facility fed basal diet (control group, n = 54) or TCM...

Solid phase synthesis of 1,3,4-oxadiazin-5(6R)-one and 1,3,4-oxadiazol-2-one scaffolds from resin-bound acyl hydrazides is described. We demonstrate here that the reactions aryl or hetero-aromatic with 2-substituted-2-bromoacetic acids 4-nitrophenyl chloroformate subsequent treatment DIEA lead to intramolecular cyclization produce six-membered 1,3,4-oxadiazin-5(6R)-ones five-membered 1,3,4-oxadiazol-2-ones, respectively. also show hydrazide-derived 1,3,4-oxadiazol-2-ones may be useful serine...

Immediate‑early response gene 5 (IER5) is a involved in the regulation of cell cycle, and its structure function have been investigated by bioinformatics analyses. The present study determined sites promoter methylation ontology (GO) annotations associated with IER5. In addition, we conducted prediction analysis to determine physical chemical properties, hydrophobicity/hydrophilicity, posttranslational modification, subcellular localization, transmembrane structure, signal peptide secondary...

A CuH-catalyzed coupling of aryl alkenes with 3-aryl-2H-azirines has been developed to synthesize optically active β,β-disubstituted ketones. We propose that this protocol occurs through a sequence in which chiral alkylcopper complex regioselectively attacks the N-C2 bonds azirines generate β-aryl imines, additionally afford ketones upon hydrolysis. This method provides novel complementary approach for synthesizing good yields high levels enantioselectivity under mild conditions.

Here we disclose a CuB-catalyzed reaction between aurone-derived α,β-unsaturated imines and styrenes to produce 2-substituted benzofuran derivatives bearing both the γ-boryl functionality α,β-unsymmetric stereogenic centers. The represents first transition-metal-catalyzed unsymmetric 1,4-Michael additions of azadienes, which would enrich arsenal CuB catalysis in organic synthesis. In addition, synthetically versatile boron-alkylated products can be elaborated by chemical transformations...

Maintaining cellular redox homeostasis is critical for cell viability and growth, with disruptions implicated in responses to chemicals drugs. This study investigates the interactions between acylfulvenes (AFs), a class of DNA alkylating drugs, thioredoxin (Trx), key regulating enzyme. AFs are semi-synthetic derivatives natural product illudin S. While their cytotoxic properties widely attributed alkylation, they also react thiols, such as Trx, implications these remain poorly understood....

The prevalence of breast cancer (BRCA) is notable in the female population, being a commonly diagnosed malignancy, where management copper levels crucial for treatment success. This research aims to explore influence homeostasis on BRCA therapy, with specific focus role Cyclin-Dependent Kinase 1 (CDK1) and its relationship regulation. A novel thermosensitive hydrogel incorporating nanoparticles (NPs) was engineered synergize chemotherapy drug vincristine (VCR) inhibiting tumor growth...