A5056385445- Evolution and Genetic Dynamics
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Mathematical and Theoretical Epidemiology and Ecology Models
- Animal Disease Management and Epidemiology
- Evolutionary Game Theory and Cooperation
- DNA and Nucleic Acid Chemistry
- Genomics and Phylogenetic Studies
- DNA Repair Mechanisms
- Microbial Natural Products and Biosynthesis
- Synthesis and Biological Evaluation
- Chromosomal and Genetic Variations
- Hemodynamic Monitoring and Therapy
- Bacterial Genetics and Biotechnology
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Toxin Mechanisms and Immunotoxins
- Plant Disease Resistance and Genetics
- Hernia repair and management
- Planetary Science and Exploration
- Genomics and Rare Diseases
- Synthesis and Catalytic Reactions
Brigham Young University
2025
Centro de Astrobiología
2015-2024
Kilimanjaro Christian Medical Centre
2020-2023
Instituto Nacional de Técnica Aeroespacial
2003-2022
Geomechanica (Canada)
2020
Institute of Seismology
2020
Consejo Superior de Investigaciones Científicas
1987-2014
Agencia Española de Medicamentos y Productos Sanitarios
2010
Centro de Biología Molecular Severo Ochoa
1991-2005
Universidad Autónoma de Madrid
1987-2005
RNA viruses replicate with a very high error rate and give rise to heterogeneous, highly plastic populations able adapt rapidly changing environments. Viral diseases are thus difficult control because of the appearance drug-resistant mutants, it becomes essential seek mechanisms force extinction quasispecies before adaptation emerges. An alternative use conventional drugs consists in increasing replication through mutagens. Here, we report about persistent infections lymphocytic...
To document fosfomycin susceptibility of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC), analyse trends in use and investigate resistance ESBL-EC isolated from urinary tract infections (UTIs). Twenty-seven Spanish hospitals participating the European Antimicrobial Resistance Surveillance Network were requested to collect up 10 sequential for centralized testing typing. EUCAST guidelines followed antibiotic testing, blaESBL type, phylogroups O25b serotype determined by PCR...
RNA viruses display high mutation rates and their populations replicate as dynamic complex mutant distributions, termed viral quasispecies. Repeated genetic bottlenecks, which experimentally are carried out through serial plaque-to-plaque transfers of the virus, lead to fitness decrease (measured here diminished capacity produce infectious progeny). Here we report an analysis evolution several low foot-and-mouth disease virus clones subjected 50 transfers. Unexpectedly, decrease, rather than...
The addition of ribavirin to alpha interferon therapy significantly increases response rates for patients with chronic hepatitis C virus (HCV) infection, but ribavirin's antiviral mechanisms are unknown. Ribavirin has been suggested have mutagenic potential in vitro that would lead "error catastrophe," i.e., the generation nonviable viral quasispecies due increment number mutant genomes, which prevents transmission meaningful genetic information. We used extensive sequence-based analysis two...
The mutant spectrum of a virus quasispecies in the process fitness gain debilitated foot-and-mouth disease (FMDV) clone has been analysed. was characterized by nucleotide sequencing three genomic regions (internal ribosome entry site; region between two AUG initiation codons; VP1-coding region) from 70 biological clones (virus individual plaques formed on BHK-21 cell monolayers) and molecular (RT–PCR products cloned E. coli ). provided statistically indistinguishable definitions with regard...
Since the introduction of quasispecies and error catastrophe concepts for molecular evolution by Eigen their subsequent application to viral populations, increased mutagenesis has become a common strategy cause extinction infectivity. Nevertheless, high complexity virus populations shown that can occur through several other pathways apart from crossing an threshold. Increases in mutation rate enhance appearance defective forms promote selection mechanisms are able counteract accelerated...
ObjectivesTo determine trends in ciprofloxacin resistance and co-resistance to other antibiotic classes blood isolates of Escherichia coli, investigate if there is an ecological relationship the community use fluoroquinolones antibiotics.
ABSTRACT Bacteriophage Lilbunny is a siphovirus infecting Mycobacterium smegmatis strain mc 2 155. It was isolated from compost of rabbit fecal matter. The genome belongs to the A6 subcluster and 50,789 bp, containing 95 open reading frames, 52.6% which encode proteins with predicted functions, three tRNA genes.
ABSTRACT Payneful, Marchy, Hami1, and Sorpresa are A1 subcluster tailed bacteriophages belonging to the Caudoviricetes class that infect Mycobacterium smegmatis strain mc 2 155. They consistent with other phages based on their genome length guanine–cytosine content. Their genomes contain six novel open reading frames.
Abstract Background When beneficial mutations present in different genomes spread simultaneously an asexual population, their fixation can be delayed due to competition among them. This interference is mainly determined by the rate of mutations, which turn depends on population size, total error rate, and degree adaptation population. RNA viruses, with large sizes high rates, are good candidates a great extent interference. To test this hypothesis, current study we have investigated whether...
ABSTRACT Evolution of fitness values upon replication viral populations is strongly influenced by the size virus population that participates in infections. While large passages often result gains, repeated plaque-to-plaque transfers average losses. Here we develop a numerical model describes evolution clones subjected to serial bottleneck events. The predicts biphasic period exponential decrease followed stationary state which display fluctuations around an constant value. This agreement...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLipophilic analogs of sparsomycin as strong inhibitors protein synthesis and tumor growth: a structure-activity relationship studyLeon A. G. M. Van den Broek, Ester Lazaro, Zbigniew Zylicz, Paul J. Fennis, Frank N. Missler, Peter Lelieveld, Marina Garzotto, D. Theo Wagener, Juan P. Ballesta, Harry C. OttenheijmCite this: Med. Chem. 1989, 32, 8, 2002–2015Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published...
Abstract Background The secondary structure of folded RNA sequences is a good model to map phenotype onto genotype, as represented by the sequence. Computational studies evolution ensembles molecules towards target structures yield valuable clues mechanisms behind adaptation complex populations. relationship between space and structures, organization at mutation-selection equilibrium, time function population parameters, presence collective effects in quasispecies, or optimal mutation rates...
The frequency of change in the selective pressures is one main factors driving evolution. It generally accepted that constant environments select specialist organisms whereas changing favour generalists. particular outcome achieved either case also depends on relative strength and fitness costs mutations across environments. RNA viruses are characterized by their high genetic diversity, which provides fast adaptation to environmental changes helps them evade most antiviral treatments....
We explore a mean-field model for the evolution of exponentially growing populations mutating replicators. Motivated by recent in vitro experiments devised to analyze phenotypic properties bacterial and viral subjected serial population transfers, we allow our silico individuals undergo unrestricted growth before applying bottleneck events. Different dynamical regimes can be mapped different experimental situations. Numerical analytical results fitness distributions calculated at...