A5006250575- Myasthenia Gravis and Thymoma
- Pituitary Gland Disorders and Treatments
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Cancer Cells and Metastasis
- Neuroblastoma Research and Treatments
- Sarcoma Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Pulmonary Hypertension Research and Treatments
- Transplantation: Methods and Outcomes
- Cancer Immunotherapy and Biomarkers
- Cardiac tumors and thrombi
- Tracheal and airway disorders
- Medical Imaging and Pathology Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunotherapy and Immune Responses
- Lung Cancer Diagnosis and Treatment
- Meningioma and schwannoma management
- TGF-β signaling in diseases
- Peptidase Inhibition and Analysis
- Vascular Malformations and Hemangiomas
- Nitric Oxide and Endothelin Effects
- Vascular Anomalies and Treatments
- Immune Cell Function and Interaction
Hôpital Marie Lannelongue
2016-2025
Centre Chirurgical Marie Lannelongue
2014-2024
Hôpital Paris Saint-Joseph
2022-2024
Institut Gustave Roussy
1999-2023
Université Paris-Sud
1999-2023
Inserm
1998-2023
Université Paris-Saclay
2019-2023
AR2i
2004-2022
Laboratoire d’immunologie intégrative du cancer
2016-2020
Immunologie intégrative des tumeurs et immunothérapie du cancer
2020
We had previously demonstrated the role of CD103 integrin on lung tumor-infiltrating lymphocyte (TIL) clones in promoting specific TCR-mediated epithelial tumor cell cytotoxicity. However, contribution intratumoral T distribution and functions prognosis significance TIL subpopulations non-small carcinoma (NSCLC) have thus far not been systematically addressed. In this study, we show that an enhanced CD103(+) subset correlates with improved early stage NSCLC patient survival increased...
Limited numbers of operated patients with chronic thromboembolic pulmonary hypertension (CTEPH) are refractory to endarterectomy (PEA) and experience persistent (PH). We retrospectively assessed lung histology available from nine PH (ineffective PEA (inPEA) group) eight transplanted for distal CTEPH inaccessible by (noPEA group). Microscopically observed peculiarities were compared the a recently developed model in piglets. Pre-interventional clinical/haemodynamic data medical history inPEA...
Abstract The worldwide incidence of pulmonary carcinoids is increasing, but little known about their molecular characteristics. Through machine learning and multi-omics factor analysis, we compare contrast the genomic profiles 116 (including 35 atypical), 75 large-cell neuroendocrine carcinomas (LCNEC), 66 small-cell lung cancers. Here report that integrative analyses on 257 neoplasms stratify atypical into two prognostic groups with a 10-year overall survival 88% 27%, respectively. We...
Abstract Malignant pleural mesothelioma (MPM) is an aggressive cancer with rising incidence and challenging clinical management. Through a large series of whole-genome sequencing data, integrated transcriptomic epigenomic data using multiomics factor analysis, we demonstrate that the current World Health Organization classification only accounts for up to 10% interpatient molecular differences. Instead, MESOMICS project paves way morphomolecular MPM based on four dimensions: ploidy, tumor...
We report novel molecular and pathologic features of sarcomas involving the heart. Intimal sarcoma appears as most frequent primary cardiac within largest described series 100 sarcomas. Immunohistochemical analysis, fluorescence in situ hybridization, real-time polymerase chain reaction, array-comparative genomic hybridization were performed on materials from 65 women 35 men, aged 18 to 82 years (mean 50 y), retrieved French Departments Pathology, between 1977 early 2013. Right left heart...
Radiation-induced lung fibrosis (RIF) is a delayed side-effect of chest radiotherapy, frequently associated with macrophage infiltration. We aimed to characterise the role pulmonary macrophages in RIF using human biopsies from patients receiving radiotherapy for thorax malignancies and model developed C57BL/6 mice after 16-Gy irradiation. High numbers (both interstitial alveolar) were detected clinical preclinical RIF. In model, upregulation T-helper (Th)2 cytokines was measured, whereas Th1...
SMARCA4-deficient thoracic sarcoma (SMARCA4-DTS) is a recently described entity with an aggressive clinical course and specific genetic alterations of the BAF chromatin remodeling complex. In present study, we reviewed pathologic features 30 cases SMARCA4-DTS, discussed its main differential diagnoses challenging diagnostic scenarios that average pathologist may face. addition, tested specificity "SMARCA4-DTS immunohistochemical signature" (co-loss SMARCA4 SMARCA2 overexpression SOX2) in...
Accumulation of CD103+CD8+ resident memory T (TRM) cells in human lung tumors has been associated with a favorable prognosis. However, the contribution TRM to anti-tumor immunity and response immune checkpoint blockade not clearly established. Using quantitative multiplex immunofluorescence on cohorts non-small cell cancer patients treated anti-PD-(L)1, we show that an increased density lymphocytes immunotherapy-naive is greatly improved outcomes. The increases during immunotherapy most...
Neuropilin-1 (Nrp-1) is a marker for murine CD4+FoxP3+ regulatory T (Treg) cells, subset of human CD4+ Treg and population CD8+ cells infiltrating certain solid tumours. However, whether Nrp-1 regulates tumour-specific CD8 T-cell responses still unclear. Here we show that defines displaying PD-1hi status lung cancer. Interaction with its ligand semaphorin-3A inhibits migration lytic function cytotoxic lymphocytes. In vivo, Nrp-1+PD-1hi tumour-infiltrating lymphocytes (TIL) in B16F10 melanoma...
Abstract Homing of CD8+ T lymphocytes to the tumor microenvironment is an important step for mounting a robust antitumor immune response. TGFβ responsible CD103 (αEβ7) integrin induction in activated intraepithelial lymphocytes. However, interplay between and their contribution T-cell infiltration activity remain unknown. Here, we used viable human lung slices autologous antigen-specific T-lymphocyte clones provide evidence that directly involved recruitment within epithelial islets...
Neoadjuvant immune checkpoint inhibitors induce major pathologic response (MPR) rates in 17- 45% of NSCLCs. We report the results a phase 2 trial neoadjuvant A for NSCLC. Patient (pts) with clinical stage IA (≥ cm)-IIIA non N2 NSCLC eligible surgery, PS 0-1, received 1 injection (1200 mg IV D1) followed by surgery between D21-D28. The primary endpoint was rate pts without toxicities or morbidities from D1 until month after surgery. Fresh tumour tissue analysed within 4 hours Response...
Background: Pulmonary arterial hypertension (PAH) is characterized by progressive distal pulmonary artery (PA) obstruction, leading to right ventricular hypertrophy and failure. Exacerbated intracellular calcium (Ca 2+ ) signaling contributes abnormalities in PA smooth muscle cells (PASMCs), including aberrant proliferation, apoptosis resistance, exacerbated migration, contractility. Store-operated Ca entry involved homeostasis PASMCs, but its properties PAH are unclear. Methods: Using a...
Inhibition of tumour necrosis factor‐α (TNF‐α), levels which are increased in the blood cirrhotic rats, prevents hyperdynamic circulatory state, mainly by decreasing vascular overproduction nitric oxide. Hepatopulmonary syndrome, is characterised intrapulmonary dilatation and alveolar to arterial oxygen tension difference ( P A-a,O 2 ), related pulmonary NO macrophages accumulated lung vessels. Since TNF‐α a potent activator macrophagic inducible oxide synthase (NOS), aim this study was...
The impact of bone morphogenetic protein receptor 2 (BMPR2) gene mutations on vascular remodelling in pulmonary arterial hypertension (PAH) is unknown. We sought to identify a histological profile BMPR2 mutation carriers.Clinical data and lung histology from 44 PAH patients were subjected systematic analysis morphometry.Bronchial artery hypertrophy/dilatation bronchial angiogenesis, as well muscular septal veins significantly increased lungs carrying mutations. found that displaying...
BackgroundMalignant Pleural Mesothelioma (MPM) is an aggressive disease related to asbestos exposure, with no effective therapeutic options.MethodsWe undertook unsupervised analyses of RNA-sequencing data 284 MPMs, assumption discreteness. Using immunohistochemistry, we performed orthogonal validation on a subset 103 samples and biological replication in independent series 77 samples.FindingsA continuum molecular profiles explained the prognosis better than any discrete model. The immune...
Abstract TGF-β is secreted in the tumour microenvironment a latent, inactive form bound to latency associated protein and activated by integrin α V subunit. The activation of latent cancer-cell-expressed re-shapes microenvironment, this could affect patient responses PD-1-targeting therapy. Here we show, using multiplex immunofluorescence staining cohorts anti-PD-1 anti-PD-L1-treated lung cancer patients, that decreased expression cell with improved immunotherapy-related, progression-free...
To characterize the prostanoid receptors on human pulmonary smooth muscle involved in vasodilatations, isolated arteries and veins were contracted with norepinephrine (10 μ M ) vessels subsequently challenged different prostanoid‐receptor agonists absence or presence of selective antagonists. Prostaglandin D 2 (PGD DP‐receptor agonist, BW245C, induced relaxations venous preparations. The pD values were: 6.88±0.11 ( n =17) 7.31±0.12 =5), respectively. relaxant responses by PGD reduced...